Yeasts & Algae

Yeast

Procedure:

Prepare yeast sample.
Dilute samples with PBS at different dilution factors.
Pipette 20 µl of solution into sample introduction port of a disposable counting chamber.
Insert loaded counting chamber into Auto M10.
Run Auto M10 software to obtain cell concentration results.

Auto M10 provides sufficient resolution for the Cellometer® software de-cluster function. It is activated for clumpy cells. As shown in Fig. 1 (b), green outlines around individual cells indicate the success of de-cluster.

Plot Yeast Concentration versus Sample Dilution

Table 1 and Figure 2 show data from a set of yeast dilution measurements. A total of 5 two-fold dilutions are used to generate the plot. The result shows excellent linearity for concentration versus sample dilution.

date time	cell type	sample name	total cell count	mean diameter	concentration
6/21/2007 15:25	Yeast_M10	Yeast -1	1324	5.5	4.62E+07
6/21/2007 15:23	Yeast_M10	Yeast -2	1087	5.5	2.53E+07
6/21/2007 15:21	Yeast_M10	Yeast -3	1482	6	1.30E+07
6/21/2007 15:19	Yeast_M10	Yeast -4	716	5.3	6.23E+06
6/21/2007 15:32	Yeast_M10	Yeast -5	355	6.5	3.12E+06
6/21/2007 15:35	Yeast_M10	Yeast -6	191	5.6	1.67E+06

Table 1. Measured yeast concentrations using the Auto M10.

Figure 2. Graph of measured yeast concentrations at various sample dilution factors.

Yeast concentrations at various sample dilution factors measured by Cellometer® AutoM10.

Join our free webinar. "Quantitative Characterization of Cells Using Automated Fluorescence Analysis" on Tuesday July 14th, 2009 @ 2pm EDT. Register now