Fast, Accurate Primary Hepatocyte Count & Viability

Cellometer provides cell sample analysis results that include: Cell count Cell concentration Cell viability Cell diameter and size distribution Cell images, and more

Benefits of Cellometer Dual Fluorescence Viability Assays: Only 20 µl of sample required, important for rare samples Improve accuracy and eliminate errors Ensure user-to-user consistency Store data automatically

Importance of Accurate Primary Hepatocyte Counts

Primary hepatocytes are regularly used for determining the toxicity of drug candidates during the drug screening process. Cytoxicity assays are plate-based assay requiring accurate cell concentration and viability measurements so that the assays can be set-up correctly. Since primary hepatocytes are generally less than 80% viable and tend to form clusters, precise and accurate cell concentration and viability measurements are crucial to obtaining reproducible, high quality data.

Characteristics of Cytoxicity experiments involving primary hepatocytes

Limitations of Manual Counting & Cellometer Solution

Challenges

Reliable concentration and viability data of primary Hepatocytes is critical for accurate analysis of compound toxicity in vitro. Due to hepatocytes' variable morphology, fragile nature and tendency to clump, traditional manual counting methods are time consuming and the subjectivity from operator-to-operator can cause inconsistent results.

Solution

Nexcelom's new method incorporates staining primary hepatocytes with a ready-to-use fluorescent dual staining solution that stains live cells with acridine orange, and dead cells with propidium iodide and then loading 20µL of labeled sample into a disposable counting chamber for analysis. Since the counting chamber is disposable, no washing is required between samples, and the risk of cross contamination is eliminated.

Fluorescent images of the stained cells are captured and using proprietary algorithms, Cellometer Vision's robust operating software accurately analyzes cell images to generate live cell count, concentration and viability percentage. Total analysis time typically takes less than 60 seconds. Cell images and all analysis data, including cell size distribution histograms, can be instantly saved for documentation. Data can also be easily exported to Microsoft Excel spreadsheets for further analysis.

Cellometer Vision is capable of analyzing many species of primary hepatocyte including:

Cell Count & Viability by Dual Fluorescence

Dual Fluorescence Viability Assay Principle

How Cellometer Vision Does Dual Fluorescence Measurement

What do I Need to Get Started?

Request a Demo One of our Applications Specialists can provide a 20-30 miniute online demo or arrange a live demo in your lab.

Ask a Specialist Our Applications Specialists are available to answer your questions.

Cellometer Vision System for Primary Hepatocytes
Instrument	Cellometer Vision, 5x Magnification
Optics Modules	VA-535-401 VA-660-501
Counting Chambers	CHT4-SD100 CHT4-PD100
Reagent Kit	Cellometer ViaStain™ AOPI Staining Solution in PBS