Automated Yeast Counting and Viability Using Cellometer
Determine yeast concentration
Determine yeast viability using fluorescent stains
- Oxonol
- Propidium Iodide
Automated Yeast Counting and Viability Applications
Ethanol fermentation
- Alcoholic beverages
- Biofuels
Yeast strain management
Yeast strain development
Research of pathogenic yeast
- Human diseases
- Crop diseases
Yeast Viability Methods
Total cells are counted in bright field, dead cells in fluorescence
% viability determined by live/total cell number:
Oxonol
A green fluorescent membrane exclusion dye that enters dead cells more readily than live cells
Propidium Iodide (PI)
A red fluorescent membrane exclusion dye that enters dead cells but not live cells
