Nexcelom Bioscience

978-327-5340

Cell Counting Tutorials
Automated Yeast Counting and Viability Using Cellometer
  • Determine yeast concentration
  • Determine yeast viability using fluorescent stains
  •   - Oxonol
      - Propidium Iodide
    Automated Yeast Counting and Viability Applications
  • Ethanol fermentation
  •   - Alcoholic beverages
      - Biofuels
  • Yeast strain management
  • Yeast strain development
  • Research of pathogenic yeast
  •   - Human diseases
      - Crop diseases
    Validation of Concentration Measurements
    Yeast Morphologies Counted using Cellometer
    Yeast Viability Methods
  • Total cells are counted in bright field, dead cells in fluorescence
  • % viability determined by live/total cell number:
  • Oxonol
  • A green fluorescent membrane exclusion dye that enters dead cells more readily than live cells
  • Propidium Iodide (PI)
  • A red fluorescent membrane exclusion dye that enters dead cells but not live cells
  • Identify Live and Dead Cells and Determine Viability
    Dynamic Range and Reliability of Viability Measurements on Cellometer Vision 10X

    Viewing: Automated Yeast Counting and Viability Using Cellometer

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