The Disposable Hemacytometer
Introduction
Over the years the hemacytometer has gone through a series of major developments creating a modern instrument that is more accurate and easier to use than its predecessors. Nexcelom’s disposable hemacytometer called CP2 is the next step forward in the evolution of hemacytometers. The CP2 slide is an easy to use enclosed chamber with counting grids for two samples. Because the CP2 slides are a single-use counting chambers they eliminate the potential issues with cross-contamination as well as improve efficiency and throughput.
How do I Count Cells with a Manual Hemacytometer?
Manual Cell Counting with Manual Hemacytometer
Step 1. Prepare the Hemacytometer
Clean hemacytometer and glass cover slip with 70% EtOH.
Step 2. Prepare Sample & Load Hemacytometer
Pipette 10 microliters of sample into the manual hemacytometer.
Step 3. Manually Count Cells in Sample
a. Place the hemacytometer under a microscope with a typical magnification of 100.
b. Focus both onto the grid pattern and the cell particles, and count the total number of cells found in 4 large corner squares
Step 4. Cell Calculations & Disposal of Hemacytometer
a. Multiply the dilution factor by the total number of cells, divide by the # of corner squares counted, and multiply by 10E4 to obtain cell concentration (cells/ml).
b. Clean hemacytometer and glass cover slip with 70% EtOH.
How do I Count Cells with a Nexcelom Disposable Hemacytometer?
Manual Cell Counting with a Nexcelom Disposable Hemacytometer
Step 1. Prepare Sample & Load Hemacytometer
Pipette 20 microliters of sample into the Nexcelom disposable hemacytometer
Step 2. Manually Count Cells in Sample
a. Place the disposable hemacytometer under a microscope with a typical magnification of 100.
b. Focused both onto the grid pattern and the cell particles, and count the total number of cells found in 4 large corner squares
Step 3. Cell Calculations & Disposal of Hemacytometer
a. Multiply the dilution factor by the total number of cells and divide by the sample volume.
b. Dispose the hemacytometer.
What Makes Our Hemacytometer Different?
- All-in-one design
- No cleaning or assembly of parts
- Two counting chambers per slide
- No washing biohazard
- Easily disposed of after use
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Details of the Nexcelom Disposable Hemacytometer
Nexcelom Bioscience’s Disposable Hemacytometer is an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates.
The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.
Nexcelom CP2 Disposable Hemacytometer
| Catalog # | Description | Unit | |
|---|---|---|---|
| CP2-001 | Box of 25 manual-count cell counting chambers, good for 50 counts. Disposable, all plastic chambers for manual cell counting with the Improved Neubauer grid pattern. Packed in microscope slide boxes, ready to use. | Box of 25 slides for 50 counts | Buy Online |
| CP2-002 | Box of 100 manual-count cell counting chambers, good for 200 counts. Disposable, all plastic chambers for manual cell counting with the Improved Neubauer grid pattern. Packed in microscope slide boxes, ready to use. | Box of 100 slides for 200 counts | Buy Online |