Nexcelom Bioscience


Tumorsphere Formation & Clonogenic Survival
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Perform tumorsphere formation and clonogenic assays to quantify size and number of formed tumorspheres.

  1. Directly image tumor spheroids in various microwell formats
  2. Non-invasive bright field imaging allows the user to image the same plate over multiple days
  3. Perform a two-color fluorescent viability assay


The Celigo imaging cytometer has been developed to fully automate imaging and analysis of tumorspheres. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.

Celigo imaging process

Image and Quantify Tumorshere Formation and Clonogenic Survival Assays

celigo software showing whole well image

Celigo software displays a plate map, image view of the whole well, and numerical data for the counted spheres. The green fill view indicates counted spheres.


Formation and Counting of Tumorspheres from Various Cell Lines

counted mda-mb-463 formed tumorspheres

Whole-well image and individually counted MDA-MB-436 formed tumorspheres (outlined in green).

counted sk-br-3 formed tumorspheres

Whole well image and individually counted SK-BR-3 formed tumorspheres (outlined in green).

counted mcf-7 formed tumorspheres

Whole well image and individually counted MCF-7 formed tumorspheres (outlined in green).

Quantification of Methylene Blue-Stained Clonogenic Colonies in 6-well Plates

MDA-MB-468 colonies stained with methylene blue

MDA-MB-468 colonies were stained with methylene blue 18 days following siRNA knockdown. The plate was imaged on Celigo at 3 um/px.

Whole-well image of control sample

whole well image MDA MB468 cells

Whole well image and counted colonies of siRNA treated sample

whole well image counted colonies siRNA

Colony number of MDA MB468 cells following siRNA knockdown

MDA-MB468 cell following siRNA knockdown