Nexcelom Bioscience

978-327-5340

Publications Referencing Celigo

Below is a list of peer reviwed journals, such as Nature, Breast Cancer Research and Journal of Cell Biology, that reference Celigo imaging cytometer.

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Author Date Title Journal Description of Cells Description of how Celigo was used
Hsu, Chih-JungOctober 2017Trans-acting oligodeoxythymidine phosphorothioate triester reagents for transient transfection optimized and facilitated by a high-throughput microbioreactor systemBiotechnology and Applied BiochemistryHeLa cellsThe Celigo was used to analyze the "the transfection efficiency of each dTtaPS-plasmid complex". Celigo captured images of GFP positive cells to determine the fluorescence intensity in each well.
Shaw, DavidSeptember 2017Development and Characterization of an Automated Imaging Workflow to Generate Clonally-Derived Cell Lines for Therapeutic ProteinsBiotechnology ProgressCHO-K1 CellsThe Celigo was used to count CHO-K1 cells stained with CellTracker Green CMFDA 0 days and 21 days after plating." Both CHO-K1 Green and CHO-K1. Red expressing CHO-K1 hosts were transfected with the same DNA preparation of mAb B" and plated into 384-well plates after electroporation. After the plates were imaged, the results were used to " drive automated hit-picking where 1,056 wells with growth were picked at random into 96-well plates."
Tsuboi, Setskuko September 2017Critical Review—Water-Soluble Near-Infrared Fluorophores Emitting over 1000 nm and Their Application to In Vivo Imaging in the Second Optical Window (1000–1400 nm)ECS Journal of Solid State Science and TechnologyHeLa cellsIn order to determine the toxicity of NIR fluorophores, the Celigo was used to determine the number of HeLa cells 0-70 hours after treatment with NIR fluorophoes.
Li, WeiSeptember 2017RDM1 gene Overexpression Represents a Therapeutic Target in Papillary Thyroid Carcinoma Running title: The new therapeutic target of papillary thyroid carcinomaEndocrine ConnectionsK1 and TPC1 cellsK1 and TPC1 cells were stained post lentivirus transduction to determine the rate of siRNA gene knockdown. 96-well plates were scanned on the Celigo using bright field and the green fluorescent channel. After gating, the Celigo determined the total cell count and " the average integrated red fluorescence intensity". The Celigo was used to create a scatter plot of "green fluorescence area (in µm2) and integrated fluorescence intensity.
Zhour, YizhouSeptember 2017Beating the Odds: The Poisson Distribution of All Input Cells During Limiting Dilution Grossly Underestimates Whether a Cell Line is Clonally-Derived or NotBiotechnology ProgressCHO-K1 cellsThe Celigo was used to image plates initially and three weeks later to determine the distribution of "mAbX expressing CHO-K1 cells [that] were transfected with either mAbX-sp1 or mAbX-sp2 vectors". 384-well plates were imaged in bright field and fluorescent channels.
Kessel, SarahSeptember 2017Real-Time Apoptosis and Viability HighThroughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image CytometerSLAS DiscoveryMCTS produced from the glioblastoma cell line U87MGThe Celigo was used to examine the "the kinetic apoptotic and cytotoxic effects" of numerous compounds of multicellular tumor spheroids. The bright field channel, blue channel, red channel, green channel, and far red channel was used on the Celigo to image and analyze the plates.
Miao, Ruoyu September 2017Utility of the dual-specificity protein kinase TTK as a therapeutic target for intrahepatic spread of liver cancer.Scientific ReportsLiver Cancer CellsThe Celigo was used to perform in situ cellular analysis on liver cancer cells after TTK inhibition. They also used Annexin V-FITC/PI Hoechst Apoptosis assay on the Celigo. They performed cell cycle analysis using BrdU and DAPI stains on the Celigo. Lastly, they used the Celigo to capture bright-field images of cell growth.
Mi Gu, Sun August 2017A synthetic cannabinoid JWH-210 reduces lymphoid organ weights and T-cell activator levels in mice via CB2 receptorsNaunyn-Schmiedeberg's Arch PharmacolSplenocytes and ThrymocytesThe Celigo was used to measure the cell viability of splenocytes after exposing cells to EthD-1 and calcein AM. It was also used to measure fluorescent intensity of splenocytes or thymocytes stained with DAPI and "secondary antibodies conjugated to AlexaFluor 488 and 594"
Briffa, RominaAugust 2017Unravelling the role of TRIB1 in colorectal cancer: a functional molecular pathology approachEPMA Journalthree stable TRIB1 knock-down CRC cell lineThe Celigo was used to monitor "cell cycle progression, proliferation, tumour growth, and invasion".
Tomita, KyokoAugust 2017Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitisJCI insightBMDM or THP-1 cellsThe Celgio was used to determine the quantity of migrated BMDMs and THP-1 cells that were fluorescently labeled.
He, XuefengJuly 2017Opa interacting protein 5 acts as an oncogene in bladder cancerJournal of Cancer Research Clinical OncologyBC-T24 and BC-5637The Celigo was used to monitor cell growth of BC-T24 and BC-5637 after infection with shOIP5/shCtl. shOIP5 and shCtl fluorescence intensity was monitored using the Celigo and the cell counts were determined from the measured fluorescence intensity. This was to determine the "knockdown of shOIP5 suppressed cell growth".
Leonidou, AndriJuly 2017Identification and Validation of Driver Kinases from Next-Generation Sequencing DataKinase Signaling Networks   
Oliemuller, ErikJuly 2017SOX11 promotes invasive growth and DCIS progressionThe Journal of PathologyMCF10A, MCF10DCIS.com, BT474, BT549, Cal148, HCC202, MX-1, and UACC893. Cells were grown in a ULA plate to allow spheroids to form. The Celigo was used to take images of the spheroids after four days. After fourteen days, the Celigo was used to determine the mammosphere-forming efficiency by determining the quantity of mamospheres and dividing by the number of cells plated per well. The Celigo was also used to measure the size of the spheroids and measure the fluorescence of Caspase-3.
Kessel, SarahJune 2017TIReal-time viability and apoptosis kinetic detection method of 3D multicellular tumor spheroids using the Celigo Image CytometerTLECytometry Part A.multicellular tumor spheroidsThe Celigo was used in combination with PI and caspase 3/7 stain to measure the rate of apoptosis and the viability of multicellular tumor spheroids
Daubeuf, FrançoisJune 2017A Fast, Easy, and Customizable Eight-Color Flow Cytometric Method for Analysis of the Cellular Content of Bronchoalveolar Lavage Fluid in the MouseCurrent Protocols in Mouse BiologyBAL cells, T cells, B cells, neutrophils, eosinophils, and macrophages 
Cherradi, SJune 2017Antibody targeting of claudin-1 as a potential colorectal cancer therapyJournal of Experimental Medicine & Clinical Cancer Researchcolorectal cancer cellsThe Celigo was used to read 6-well plates that contained colorectal cancer cells using the single colony verification application. The tumorsphere application was also used and captured images of the tumorspheres. Also, the expression analysis assay was used to determine the fluorescent signals. During this experiment, they stained the cells with DAPI nuclear stain. Lastly, the Celigo was used to determine the apoptosis and growth rate of the tumorspheres.
de Andrade Mello, Paola June 2017Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell deathOncotarget, Advance PublicationsMCA38 colon cancer cellsThe viability was determined after the cells were exposed to CCK-8 and the Celigo was used to capture images of the live cells.
Singh, VeenaJune 2017Performance of Biocept's sample collection for tumor cell analysis.Tumor BiologyBT474 (HER2 amplified) and H3112 (ALK re-arranged) cellsThe Celigo was used to count BT474 (HER2 amplified) or H3112 (ALK re-arranged) cells after being thawed.
Nabbi, ArashMay 2017ING3 promotes prostate cancer growth by activating the androgen receptorBMC MedicineLNCaP, PC3, and DU145 cellsThe Celgio was used to monitor cell proliferation of LNCaP, PC3, and DU145 cells in a 96-well plate.
Kondo, YasushiMay 2017Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cellsDiabetologiahiPSC lines: 648A1 and 692D2. Fibroblast-derived hiPSC lines: 409B2, 206B6 and 201B7. hESC lines: H9, KhES1 and KhES3.Cells were immunostained with anti-insulin antibodies and the Celigo was used to determine the induction rate of INS+ cells.
Huang, YingMay 2017Identification of hMex-3A and its effect on human bladder cancer cell proliferationOncotargetBladder cancer cells lines:5637 and T24The Celigo was used to examine the proliferation after bladder cancer cells were transfected with an interference RNA sequence.
Grav, Lise May 2017 Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO CellsSpringer LinkCHO CellsThe Celigo was used to determine the cell survival and cell confluency.
Ivanov, Delyan May 2017 High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase ActivitySpringer Link References the Celigo as being capable of measuring spheroid size and volume in high throughput.
Chan, LeoMay 2017A high-throughput image cytometry-based screening method for the detection of IL2-induced peripheral blood mononuclear cell-mediated cytotoxicityCytotherapyperipheral blood mononuclear cells (PBMC)"Viability was determined by employing different staining techniques such as enzymatic stain, nucleic acid stain, multi-stain method, fluorescent stain, and trypan blue staining on the Celigo and Cellometer. "
Chan, Leo May 2017Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image CytometrySpringer Link In this work, they "demonstrate a novel high-throughput cytotoxicity screening assay using the Celigo imaging-cytometry method". A 96 well plate was used with the Celigo to analyze the percent lysis of cells.
Cuny, GregoryApril 2017PYRIMIDINE COMPOUNDS AND METHODS USING THE SAMEUnited States Patent Application SH-SY5Y-hTAU441 cellThe Celigo was used to assess the cytotoxicity of SH-SY5Y-hTAU441 cells.
Gheller, BrandonApril 2017PYY regulates human skeletal muscle progenitor cell proliferationThe FASEB JournalSkeletal Muscle stem cells (satelitte cells)The Celigo was used to track percent confluence of cells over five days.
Blum, JamieApril 2017Short-term Inflammation Increases Proliferative Capacity of Human Skeletal Muscle Progenitor Cells from Young and Old Female DonorsThe FASEB JournalMuslce Progenitor CellsThe Celigo was used to track the percent confluence, number of nuclei, and percent cell death.
Smith, PaulApril 2017Microenviroment CytometrySingle Cell Analysis: Contemporary Research and Clinical Applicationsmulticellular tumor spheroids (MCTSs)The Celigo was used to track the multicellular tumor spheroids health by staining with viability dye DRAQ7. They also looked at non-viable cells in the culture.
Zhang, HaohaiApril 2017Novel high-throughput cell-based hybridoma screening methodology using the Celigo Image CytometerElsevierChinease hamster ovary (CHO) cells"Demonstrate novel high-throughput screening methodology employing the Celigo Image Cytometer, which avoids nonspecific signals by contrasting antibody binding signals directly on living cells, with and without recombinant antigen expression."
Venugopal, JessicaApril 2017Ouabain promotes partial epithelial to mesenchymal transition (EMT) changes in human autosomal dominant polycystic kidney disease (ADPKD) cellsElsevierkidney disease (ADPKD) cellsThe Celigo was used in combination with an inverted microscope to monitor the wound healing of a cell monolayer.
Yang, Mei-LinMarch 2017"IL-6 ameliorates acute lung injury in influenza virus infection"Scientific Reportsfibroblasts isolated from IL-6 -/- mice and WT miceFibroblasts were isolated from IL-6-/- mice and WT mice. The Celigo was used to determine the cell count, doubling time, and apoptosis rate by immunofluorescence with anti-cleaved caspase-3 antibody. It was also used to monitor the number of virus infected cells engulfed by macrophages compared to IL-6/- macrophages. The macrophages were exposed to QD649 particles and stained with DAPI.
Itoh, M March 2017 NanoCulture Plate: A scaffold‐based high‐throughput three‐dimensional cell culture system suitable for live imaging and co‐cultureTechnology Platforms for 3D Cell Culture: A User's Guide  States how NCP can be used with the Celigo for measurments.
Slawny, NicoleMarch 2017Physiologically relevant spheroid models for three‐dimensional cell cultureTechnology Platforms for 3D Cell Culture: A User's Guide  Lists the Celigo as a type of automated 3D assay equipment
Quentin Li, QingdiMarch 2017Proteomic analysis of proteome and histone post-translational modifications in heat shock protein 90 inhibition-mediated bladder cancer therapeuticsScientific Reports5637 bladder cancer cellsThey wanted to determine the antitumor impact of HSP90 inhibitors in 5637 bladder cancer cells. They stained the cells and used the Celigo to examine the cell viability.
Dall'Acqua, WilliamJanuary 2017Enhanced tumor-targeting selectivity by modulating bispecific antibody binding affinity and format valenceScientific ReportsNCI-H358 non-small cancer cellsCell cytotoxicity studies were performed on a Celigo S Imaging cytometer. Overlay of brightfield with either green fluorescence or blue fluorescence identified and qualified CMFDA or BMQC positive cells using Celigo Image processing software.
Li, LinfengJanuary 20173D High-Content Screening of Organoids for Drug DiscoveryElsevierNoneThis paper listed the Celigo as a 3D high content screening system
Parker, ChristopherJanuary 2017Ligand and Target Discovery byFragment-Based Screening in Human CellsElsevierHuman Cells
Cribbes, ScottJanuary 2017A Novel Multiparametric Drug-Scoring Method for High-Throughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image CytometerSAGE Journalsglioblastoma cell line U87MGIn this work, we employed the Celigo Image Cytometer to examine the effects of 14 cancer drug compounds on 3D MCTS of the glioblastoma cell line U87MG in 384-well plates.
Auberon, FlorenceDecember 2016Isolation of novel stilbenoids from the roots of Cyrtopodium paniculatum (Orchidaceae)Fitoterapiahuman glioblastoma U-87MG cellsCeligo
Braganza, AndreaDecember 2016UBE3B is a calmodulin-regulated, mitochondria-associated E3 ubiquitin ligaseThe Journal of Biological Chemistryhuman cellsThe cells were counted various times after plating. Colonies were also stained with Crystal violet dye and counted with the Celigo.
Boss, OlivierDecember 2016Methods and compositions for inducing differentiation of human brown adiopocyte progenitorsFPOadipose tissue (BAT) progenitor cellsCells were exposed to C1-BODIPY® 500/510 C12 (Molecular Probes #D-3823) and incubated for 3 to 6 hours. C1-BODIPY® 500/510 C12 is a fluorescent fatty acid analog that is sometimes utilized for lipid trafficking studies (ThermoFisher). Next, the cells were analyzed in bright field and fluorescence. They looked at the total fluorescent intensity per well.
Wu, YaoNovember 2016Cucurbitacin-I induces hypertrophy in H9c2 cardiomyoblasts through activation of autophagy via MEK/ERK1/2 signaling pathwayToxicology LettersH9c2 cellsThe cell viabilities were detected by the Celigo Image Cytometer
Kramer, DanielaNovember 2016Strong antitumor synergy between DNA crosslinking and HSP90 inhibition causes massive premitotic DNA fragmentation in ovarian cancer cellsCell Death & Differentiationhuman ovarian cancer cell lines 
Su, XNovember 2016TAp63 suppresses mammary tumorigenesis through regulation of the Hippo pathwayOncogenemammary epithelial cells (MECs), mammary stem cells (MaSCs) and tumor-initiating cells (TICs) 
Wu, YaoNovember 2016Cucurbitacin-I induceshypertrophy in H9c2 cardiomyoblasts through activation of autophagy viaMEK/ERK1/2 signaling pathwayToxicology LettersH9c2 cellsThe Celigo was used to analyze cell viability of H9c2 cells
Cisneros Castillo, LilianaOctober 2016A novel computer-assisted approach to evaluate multicellular tumor spheroid invasion assayScientific Reportstumor and peripheral cellsTo quantify the size of MCTS in invasion assays, the present of the Celigo Cytometer is an approach that comes already with a device that takes images automatically of an inserted plate.
Kari, VijayalakshmiSeptember 2016Loss of CHD1 causes DNA repair defects and enhances prostate cancer therapeutic responsivenessThe Embo JournalProstate cancer cells 
Hamilton, DuaneSeptember 2016Brachyury, a vaccine target, is overexpressed in triple- negative breast cancer Society for EndocrinologyTriple-negative breast cancer cellsNuclei were stained using DAPI and images were acquired using the Celigo S cell Imaging Cytometer
Potapova, TamaraAugust 2016Transcriptome analysis of tetraploid cells identifies cyclin D2 as a facilitator of adaptation to genome doubling in the presence of p53Molecular Biology of the CellTetraplid cellsMulti-well plates with labeled cells were imaged on Celigo Imaging Cytometer. Images were quantified and each image typically contained several hundred of cells; 3-4 images were analyzed to determine the mean number of positive cells and the standard deviation
Imamura, YukioAugust 2016Near-Infrared Emitting Pbs Quantum Dots for in Vivo Fluorescence imaging of the Thrombotic state in septic mouse brainMoleculesHeLa cellsHeLa cells were plated at 6000 cells/well in 96 well plates. After culturing overnight, QD1100 was added to every well at a final concentration of 0-50 nM. The number of cells in each well was counted with the Celgio after 0,7,24,4,8, and 60 hours.
Maguire, SarahAugust 20163D modelling identifies novel genetic dependencies associated with breast cancer progression in the isogenic MCF10 modelThe Journal of PathologyMCF10A cellsComparison of pathway aberrations associated with progression identified that when cells are grown as 3D spheroids, they show perturbations of cancer-relevant pathways. The spheroids are was calculated using the Celigo S.
Chan, Leo Li-YangAugust 2016A high-throughput AO/PI- based cell concentration and viability detection method using the Celigo image cytometryCytotechnologyJurkat cellsThe high-throughput AO/PI method described here allows for 96 well to 384-well plate samples to be analyzed in less than 7 mins, which greatly reduces the time required for the single sample based automated cell counter.
Shan, FengJuly 2016Investigation of cancer drug penetration in 2D and 3D tumor cell culture modelsUniversity of PittsburghCal33 HNSCC cells 
Bonasu, SurekhaJuly 2016Real-time Caspase 3/7 measurement of suspension and adherent cells using the Celigo Image cytometerPosterJurkat cellsMonitoring by detecting a green caspase 3/7 signal but also perform an end point assay by counterstaining the cells with Hoechst and therby determined a percent nucleated cells that are apoptosis by imaging on the Nexcelom Celigo image cytometer.
Chan, Leo Li-YangJuly 2016A high-throughput image cytometry-based screening method for the cytotoxic effect of drug compounds on 3D tumor spheroidCancer ResearchCancer cellsFive experiments were conducted demonstrating comparable results in 2D and 3D models using the image based high-throughput screening method for 3D tumor spheroids on 384- well ultra low attachment round bottom microplates using the Celigo image cytometer.
Shirihai, Orian July 2016Lysosome acidification by photoactivated nanoparticles restores autophagy under lipotoxicityThe Journal of Cell BiologyB- cellsAnalysis parameters for images acquired by the Celigo were optimized to identify individual cells based on fluorescence. Cells were then fixed in 4% paraformaldehyde, stained with DAPI, and imaged using Celigo to count the total number of cells per well for normalization.
Chan, Leo Li-Ying June 2016High-throughput 3D tumor spheroid screening method for cancer drug discovery using Celigo Image CytometryJournal of Laboratory AutomationU87MGCeligo was used to determine the seeding density for the tumor sphere formation of the cell line U87MG, and it was to measure the IC50 value, and the dose responses of 17-AGG, paclitaxel, TMZ and doxorubicin
Ellegaard, Anne-Marie June 2016Repurposing Cationic amphiphilic antihistamines for cancer treatmentEbioMedicineNon-small cell lung cancer (NSCLC) Celigo measured the cell death after 15 minutes of propidium iodide and Hoechst-33342 staining was employeed at 37 degrees celcius.
Mazor, Yariv June 2016Enhancement of Immune Effector Functions by modulating IgG's intrinsic affinity for target antigenImmune cells, T-cellsImmune cells, T-cellsTarget cells were stained with Calcein AM dye, and then seeded in a 96 well plate at a density of 1x10^4 cells/well in RPMI in 1640 with GlutaMAX with 5% FBS. Calcein AM positive cells were quantified by Celigo.
Napoli, Marco June 2016ΔNp63/DGCR8- Dependent MicroRNAs mediate therapeutic efficacy of HDAC inhibitors in CancerCancer cellSquamous cell carcinomas and lymphoma cellsTo acess the efficacy of the compounds in affecting the protein levels of ΔNp63 and DGCR8, evaluating cells by immunofluoresence and quantified them with Celigo.
Patterson, Lisa May 2016In vitro assays for endothelial cell functions required for angiogenesis: Proliferation, motility, tubular differentiation, and matrix proteolysis Angiogenesis ProtocolsUmbilical vein endothelial cells, microvascular endthothelial cellsLysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Kildegaard, Helene Faustrup May 2016Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment Biotechnology & BioengineeringCHO cellsLysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Tomokazu, TanakaApril 2016Low-dose farnesyltransferase inhibitor suppresses HIF-1a and snail expression in Triple-negative breast cancer MDA-MB-231 cells in vitroJournal of Cellular PhysiologyMDA-MB-TNBC and MDA-MB-231 cellsTumorspheres were directly counted by the Celigo Cell Cytometer at a 12-day culture. Pictures of each well were taken to conform spheres and aggregates of cells.
Mattson, EmmaApril 2016Understanding the role of RB-binding protein KDM5A in cancer cell proliferationIllinois Mathematics and Science Academylung cancer cellsFocused on clarifying the role of KDM5A in a non-small cell lung cancer line under different concentrations of erlotinib, a common chemotherapy drug, by examinging the cell cycle and cell proliferation using the Celigo.
Elisabeth, Corecelle-TermeauApril 2016Excess sphingomyelin disturbsATG9A trafficking and autophagosome closureAutophagySMPD-1- deficient cellsLysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Amy, WagersApril 2016Methods and compositions forrejuvenating skeletal muscle stem cellsFPOSkeletal muscle stem cellsWells containing myogenic colonies were either conunted by brightfield microscopy or fixed with 4% PFA and the number of cells per well was counted on a Celigo automated microscope as Hoechst-stained nuclei at the indicated time points
Gayathri, DeviApril 2016Three-Dimensional culturesystems in cancer research: Focus on tumor spheroid modelPharmacology & TherapeuticsCancer stem cellsCancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically relevant cell-cell and cell-matrix interactions, gene expression and signaling pathway profiles, heterogeneity, and structural complexity that reflecdt in vivo tumors.
Bidisha, BhattacharyaApril 2016Fine-tuning of macrophageactivation using synthetic rocaglate derivatesScientific ReportsJ7-21 cellsCeligo cytometer was used ot measure number of GFP-Ipr1 positive cells and GFP-Ipr1 fluorescence intensity per nucleus after counterstaining with nuclear stain DAPI.
Peitzsch, ClaudiaMarch 2016An epigenetic reprogramming strategy to re-sensitize radioresistant prostate cancer cells Cancer Research Cancer stem cellsAfter 14 days, cells were anaylzed and automatically scanned using the Celigo for 30 mins at 37 degrees celcius, washed with PBS at the end of the culture, then analyzed by the Celigo.
Stanford, ElizabethMarch 2016The role of the aryl hydrocarbonreceptor in the development of the cells with the molecular and functionalcharacteristics of cancer stem- like cellsBMC BiologyBreast cancer stem cellsAfter cells were treated, harvested, dosed, and plated colonies were quantified with the Celigo after eight days.
Mohammad, TariqJanuary 2016Eukaryotic translationinitiation factor 5A (elF5A) is essential for HIF-1a activiation in hypoxiaBiochemical and Biophysical research communicationsTumor spheroidsSuppression of elF5A by siRNA oligomediated knockdown or treamtent with GC7, a deoxyhypusine synthase inhibitor, led to significant reduction of HIF-1a activity
Edwin, ChangNovember 2015AshwaMAX and withaferin Ainhbits gliomas in cellular and murine orthotopic modelsJournal of Neuro-oncologyGBM2, GBM39Human parietal-cortical gliobastoma cells (GBM2, GBM39) were isolated from primary tumours while U87-MG was obtained commerically.
Linfeng, LiNovember 2015High-throughput imaging:Focusing in on drug discovery in 3DMethodsMCT cells3D organotypic culture models such as organoids and multicellular tumor spheroids (MCTS) are becoming more widely used for drug discovery
Henning, Gram HensenDecember 2015Versatile microscale screeningplatform for improving recombinant protein productivity in chinese hamsterovary cellsScientific ReportsOvarian cellsThe platform consists of four techniques compatible with 96-well microplates:lipid-based transient transfection, cell cultivation in microplates, cell counting and antibody-independent product titer determination based on split-GFP complementation
Sivapriya, PonnurangamDecember 2015Quinomycin A targets notchsignaling pathway in pancreatic cancer stem cellsOncotargetCSC cellsQuinomycin treatment resulted in significant inhibition of proliferation and colony formation in pancreatic cancer cell lines, but not in normal pancreatic epithelial cells
Annika, BaudeDecember 2015Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombinationNucleic Acids researchHeLa cervic carcinoma cellsPOGZ-depleted cells show a similar defect in DNA damage-induced RPA2 phosphorylation as HDGFRP2-depleted cells
Andrew, CampbellOctober 2015Mutation ofataxia-telangiectasia mutated is associated with dysfunctional glutathionehomeostasis in cerebellar astrogliaGliaMouse cellsCerebellar atroglia isolated from Atm mutant mice show decreased expression of the cystine/glutamate exchanger subunit xCT, glutathione (GSH) reductase, and glutathione-S-transferase.
Thilo, RiedlOctober 2015High-throughput screening forinternalizing antibodies by homogeneous fluorescence imaging of apH-activated probeJournal of Biomolecular ScreeningA431, AU565, SKOV-3 cellsThe pH-activated CypHer5E label becomes fluorescent upon internalization into the acidic environment of endocytic organelles, whereas background fluorescence of noninternalized CypHer5E is minimal.
Narendran, AruSeptember 2015Targeted inhibition of MEK1 bycobimetinib leads to differentiation and apoptosis in neuroblastoma cellsBioMed centralNone
Simeonov, AntonSeptember 2015High- throughput fluorescence imaging approaches for drug discovery using in vitro and in vivo three-dimensional modelsExpert Opinion Drug DiscoveryNone
Yoshida, MinoruSeptember 2015Identification of thedeterminants of 2-deoxyglucose sensitivity in cancer cells by shRNA libraryscreeningScienceDirectCancer cells
Kaji, KeisukeSeptember 2015Reprogramming roadblocks are system dependentISSCR(iPSC's)
Anant, ShrikantAugust 2015RNA binding protein RBM3increases B-catenin signaling to increase stem cell characteristics incolorectal cancer cellsWiley Online LibraryCancer stem cells
McGlennen, RJuly 2015Comparison of Antimicrobial andwound healing agents on oral fibroblast viability and In-vivo bacterial loadOmics OnlineGingival fibroblast cells
Dobbelstein, MatthiasJuly 2015MicroRNA-101 suppresses tumorcell proliferation by acting as an endogenous proteasome inhibitor viatargeting the proteasome assembly factor POMPScienceDirectTumor cells
Dubrovska, AnnaJune 2015Development of novelradiochemotherapy approaches targeting prostate tumor progenitor cells usingnanohybridsInternational Journal of CancerCancer stem cells
Gahl, WilliamJune 2015Automated, High-throughputderivation, characterization and differentiation of induced pluripotent stemcellsNature Methods(iPSC's)
Mermod, NicolasJune 2015Epigenetic regulatory elements:Recent advances in understanding their mode of action and use for recombinantprotein production in mammalian cellsBiotechnology Journalmammalian cells
Lee, DanMay 2015High- throughput direct cell counting- based natural killer cell mediated- cytotoxicity assay using Celigo Imaging Cytometry The Journal of Immunology NK cells
Sun, YiMay 2015A reliable parameter tostandardize the scoring of stem cell spheresPLOS oneMouse embryonic stem cells
Jaattela, MarjaMay 2015Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assayAutophagy 
Chang, StephenMay 2015Rapid and efficient generationof transgene-free iPSC from a small volume of cryopreserved bloodStem cell reviews and reportshESConline
Kildegaard, HeleneApril 2015One-step generation of tripleknockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichmentBiotechnology JournalCHOOnline
Leach, M.OMarch 2015Acquired resistance to EGFRtyrosine kinase inhibitors alters the metabolism of human head and necksquamous carcinoma cells and xenograft tumorsBritish Journal of CancerCAL/PJ HNSCConline
Nabergoj, Dominik, Elsa December 2014Evaluation of anti-inflammatory and proapoptotic activities of synthetic clathrodin analogues in human THP-1 monocytic Leukemia cells Thesis THP-1 cells online
Flores, ElsaNovember 2014Non-disruptively count and quantify fluorescence iPS colonies during 2 degree reprogramming: 7 min per 6 well plate, dual- fluorescence whole well imaging cytometry NatureMouse embryonic stem cells(G4) online
Kolev, VihrenNovember 2014P13K/mTOR dual inhibitor VS-5584preferentially target cancer stem cellsAmerican Association for Cancer ResearchCancer stem cellsTo detemine tumorsphere forming efficency, cells from the tissue culture were plated and enumerated using the Celigo.
Karlin, MondalNovember 2014The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressorCell ReportsBT549 and MDA-MB231-LM2 breast cancer cellsCeligo was used to perform cell proliferation assays.
Westbrook, ThomasNovember 2014The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressorCell ReportsNoneonline
Miura, HiromiOctober 2014Development of spheroid based high-throughput screening of cell-cell adhesion inhibitors to reverse acquired multicellular resistance Cancer Research A549 online
Randhawa, ZengOctober 2014Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugsAntiviral ResearchHEK293, COS7Cell counts were evaluated by Celigo to ascertain cytotoxicity of compounds.
Glicksman, Marcie A.October 2014Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugsAntiviral ResearchHEK293, COS7online
Landmann, ProiaSeptember 2014UDP glucuraonosyltransferase 1Aexpression levels determine the response of colorectal cancer cells to theheat shock protein 90 inhibitor ganetespibCell Death and Diseasehuman colorectal cancer cell linesCell confluence in drug treated and untreated wells was determined by Celigo with bright field.
Fu, CreightonSeptember 2014Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinaseBreast Cancer ResearchMCF-7L, BT483, and T47D (human breast cancer)Cell count was assessed on Celigo via methylene blue and bright field.
Schiff, RSeptember 2014Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinaseBreast Cancer ResearchMCF7L (Human Breast Cancer), BT483, T47DOnline
Xu, Farach-CarsonJuly 2014Three-dimensional in vitro tumormodels for cancer research and drug evaluationBiotechnology Advancestissue engineered-3D tumor modelsCeligo measured spheroid diameter, permieter, and area which enabled assessments of motility, angiogenesis, and matrix invasion, as well as the actions of cell growth inhibitors.
Brix, RafnJuly 2014Screening and identification ofsmall molecule inhibitors of ErbB2-induced invasionMolecular OncologyMCF-7 (human breast cancer) and SK-OV3 (human ovarian)Cells were stained with Hoechst and PI and Celigo ascertained the total cell counts as well as number of dead cells.
Jayanthan, RuanJuly 2014Aurora kinases as druggabletargets in pediatric leukemia: heterogeneity in target modulation activitiesand cytotoxicity by diverse novel therapeutic agentsPLOS Onepediatric and infant leukemia cell linesCell lines were stained with Alamar blue to assess cells survival via metabolic activity.
Scheerlinck, Van SteendamJune 2014Detailed method description fornoninvasive monitoring of differentiation status of human embryonic stemcellsAnalytical Biochemistryhuman embryonic stem cells (hESCs)Celigo evaluated colony confluence and measurement of stress level via Oct 4 expression.
Pederson, RondaMay 2014Accelerating genome editing inCHO cells using CRISPR Cas9 and CRISPy, a web-based target finding toolBiotechnology and BioengineeringCHO cellsTwo-channel bright field and GFP images were captured by Celigo to count cells and determine transfection efficiency.
Lund, KildegaardMay 2014A versatile system for USERcloning-based assembly of expression vectors for mammalian cell engineeringPLOS OneCHO-S (Chinese hamster ovary clonal isolate)Cells were plated in 6-well plates and transfected with GFP-tagged vectors before the addition of Hoechst stain. Two-channel (Hoechst and GFP) images were collected on the Celigo to determine cell count and transfection efficiency.
Guo, LohMay 2014Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreeningMolecular PharmaceuticsMCF-7 (human breast cancer)Bright field images of the spheroids in 96-well plates were generated over a time course by Celigo. Growth kinetics of spheroid diameter, perimeter, and area were captured.
Jaiswal, LauritzenMay 2014S100A11 is required forefficient plasma membrane repair and survival of invasive cancer cellsNature CommunicationsMCF-7 (human breast cancer)Cell death was determined by Celigo using propidium idodide (PI) 96 hours after control or siRNA delivery.
Yang, ChungMay 2014Systems analysis of the prostatetumor suppressor NKX3.1 supports roles in DNA repiar and luminal celldifferentiationF1000 ResearchLNCaP (human prostate cancer)Cells were dual stained with Heochst and Alexa Fluor 594 and imaged by Celigo to ascertain cell proliferation.
Guo, LohMay 2014Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreeningMolecular Pharmaceuticsmulticellular tumor spheroidsCeligo captured bright field images over time to determine diameter, perimeter, and area of the spheroids with or without drug treatment.
Kalineo, TheinApril 2014Acetaminophen and NAPQI aretoxic to auditory cells via oxidative and endoplasmic reticulumstress-dependent pathwaysHearing ResearchHEI-OC1 cells (murine-derived auditory cells)Celigo performed direct cell counts after treatment with control or drug over a time course.
Spike, KelberApril 2014CRIPTO/GRP78 signaling maintainsfetal and adult mammary stem cells ex vivoStem Cell ReportsMCF10A and fetal mammary stem cellsCeligo measured DNA content via Hoechst and cell proliferation through bright field images.
Vissapragada, ContrerasMarch 2014Bidirectional crosstalk betweenperiventricular endothelial cells and neural progenitor cells promotes theformation of a neurovascular unitBrain ResearchPeriventricular region endothelial cells (PVEC) and neural progenitor cells (NPC)Cells were imaged by Celigo in 96-well Matrigel coated plates to ascertain the length and pixel intensity of 3D cords created by GFP-labeled ECs.
Holembowski, KramerMarch 2014TAp73 is essential for germ celladhesion and maturation in testisJournal of Cell BiologyGerm-Stertoli cell co-culturesCeligo captured two-channel images (bright field and GFP) to quantify cells after lentiviral infection of adhesion genes.
Emhemmed, AzouaouMarch 2014Selective anticancer effects ofa synthetic flavagline on human Oct4-expressing cancer stem-like cells via ap38 MAPK-dependent caspase-3-dependent pathwayBiochemical PharmacologyNT2/D1 (human embryonal carcinoma)Celigo determined the rate of apoptosis via Annexin V/PI staining after flavagline derivative FL3 application.
Trapnell, CacchiarelliMarch 2014The dynamics and regulators ofcell fate decisions are revealed by pseudotemporal ordering of single cellsNature Biotechnologyhuman skeletal musucle myoblastsCeligo performed whole well imaging with Hoechst and markers to determine the number of cells or nuclei, fraction of nueclei in MYH2- or CD13-positive cells, and total MYH2-positive area.
Shih, Chung-HsuanFebruary 2014Astroglial-Derived PeriostinPromotes Axonal Regeneration after Spinal Cord InjuryThe Journal of NeuroscienceRat NeuritesNeurite length was measured using Celigo cytometer to quantify the confluence of TujI-stained neurites, providing a quantitative measurement of neurite outgrowth.
Fu, MorrisonFebruary 2014Therapeutic potential of thedual EGFR/HER2 inhibitor AZD8931 in circumventing endocrine resistanceBreast Cancer Research TreatmentTamoxifen resistant (TamRes) MCF-7 and T47D cells (both breast cancer)Cells counts and rate of apoptosis via Annexin V-FITC staining was determined by Celigo.
Schulz, RamonaJanuary 2014HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancerCell Death and DiseaseMDA-MB-453, and SK-BR-3 (Human Breast Cancer)Cells were seeded and cultured for 1 day, then treated with CP724.714 (CP) for 24hr or left untreated and followed up to day 5. Cell confluence measured daily by Celigo. For cell survival, equal numbers of treated or untreated cells were plated into 12 well plates. With the Celigo cytometer, cell confluence was measured over indicated time periods w/Celigo
Sproul, AndrewJanuary 2014Characterization and MolecularProfiling of PSEN1 Familial Alzheimers Disease iPSC Derived NeuralProgenitorsPLOS oneFibroblast 11 and 11C, reprogramed to iPSCsImmunostaining was performed. Hoechst was used to visualize DNA. The following antibodies were used: OCT4, SSea4, Nanog, Tra-160, Ki67, MAP2, Nestin, NeuNm TujI, NLRP2, NDP. Quantification of immunostaining was done on the Celigo 200-BFFL
Giovannini, MarcoJanuary 2014mTORC1 Inhibition Delays Growthof Neurofibromatosis Type 2 SchwannomaNeuro-OncologyHuman Primary Schwann cellsCells were fized in 4% paraformaldehyde and stained with anti-S100 protein,, followed by AI549-conjugated goat anti-rabbit secondary antibody and counterstained with Hoechst 33258. Cell surface areas were determined using the automated Celigo Cytometer.
Postupalenko, ViktoriiaJanuary 2014Intracellular Delivery ofFunctionally Active Proteins Using Self-AssemblingPyridulthiourea-polyehtlenimineJournal of Controlled ReleaseU87 (Human Glioma), CaSki (Human small bowel mesentery), HeLa (Human Cervical Cancer)Cells were seeded into 96-well plates at 10 4 cells/well the day before . Flow cytometry analysis was performed with a Celigo Cytometer after a 24 h incubation period on a suspension of freshly trypsinized cells
Postupalenko, SiblerJanuary 2014Intracellular delivery offunctionally active proteins using self-assemblingpyridylthiourea-polyethylenimineJournal of Controlled ReleaseU87 (human primary glioblastoma)Cells were plated in 96-well plates and treated with GFP polyplexes. Celigo was used to determine intracellular eGFP delivery.
Schulz, R.January 2014HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancerCell Death and Disease
Ferree, Andrew2014Supplemental Material-characterization and molecular profiling of PSEN1Landes Bioscience
Venkatanarayan, Raulji2014IAPP-driven metabolicreprogramming induces regression of p53-deficient tumours in vivoNatureH1299 (human lung adenocarcinoma)Rates of apoptosis (via Annexin V/PI staining) and ROS generation (via CellRox Deep Red) were determined by Celigo.
Chen, Hsing-YuMarch 2013Inhibition of red/Fyn/c-CBLPathway Function by Cdc42 Controls Tumour Initiation Capacity and TamoxifenSensitivity in Basal-like Breast Cancer CellsEMBO Molecular MedicineMDA-MB-231, MDA-MB-468, Hs578T, HCC1569, MCF-7m HCC38, HCC70, HCC1954, MCF-10A (Human Breast Cancer)Cells were incubated with Calcein AM and PI for 15 mins, after which live and dead cells were counted utilizing the Celigo Cytometer. Levels of intracellular stress of oxidation were determined by CMH2DCFDA staining to the manufacturers instructions. Briefly cells were incubated with CM-H2DCFDA in the phenol red free medium in teh dark for 30 min, after which cells were washed once and fluorescent intensity of cells was determined using Celigo.
Proschel, ChristophDecember 2013Delayed Transplantation ofPrecursor Cell-Derived Astrocytes Provides Multiple Benefits in a Rat Modelof ParkinsonsEMBO Molecular MedicineCortical and dopaminergi mid brain neurons isolated from E18.5 Sprague-Dawley rats.Cell survival was determined by co-labelling cultures with TujI and anti-tyrosine hydroxulase antibody. TujI+ or TujI+/TH+ neurons were counted using a Celigo Cytometer.
Neradugomma, NaveenDecember 2013Prolactin Signalling EnhancesColon Cancer Stemness by Modulating Notch Singaling in a Jak2-STAT3/ERKMannerCarcinogenesisColon cancer cell LinesHT29, HCT116, SW480, SW620, DLD1, and normal intenstinal epithelial fetal human colon (FHC) cellsColosphere formation was assessed after 4-6 days, the number and size of colonospheres was determined using Celigo
Xu, CongNovember 2013A Zebrafish Embryo CultureSystem Define Factors that Promote Vertebrate Myogenesis Across SpeciesCellZebrafish cellsZebra fish cells were iamged after 2 days using a Celigo cytometer for GFP, mCherry and BR signals.
Neradugomma, N.November 2013Prolactin induces Notchsignaling in a Jak2-STAT and Jak2-ERK pathway in colon cancer stem andprogenitor cellsCarcinogenesis
Zangi, LiorSeptember 2013Modified mRNA Directs the Fateof Heart Progenitor Cells and Induces Vascular Regeneration After MyocardialInfarctionNature BiotechnologySkeletal muscle myotubes differentiated from primary mouse satellite cellsCells were harvested and equal numbers were plated in each well of a 96-well plate in growth media. Cells were transfected with modified RNA after 3 d in differentiation media. Myotubes were transfected w/0, 0.3, 1 or 3 µg of GFP-modified RNA. Cells were fixed 16 h after transfection. Transfected myotubes were stained for skeletal muscle myosin heavy chain, 10 µg/ml Hoechst and rabbit anti-GFP Alexa-488. Pictures were taken from the whole well using the Celigo cytometer under blue, red and green channels. Cell viability was measured by quantifying the total number of nuclei in the transfected wells 16 h after transfection and normalizing them to the total number of nuclei in the non-transfected wells.
Chen, Hsing-YuSeptember 2013MEK1/2 Inhibition SuppressesTomixfen Toxicity on CNS Glial Progenitor CellsThe Journal of NeuroscienceO-2A/OPC (Astrocyte progentior/oligodendrocyte precursor cells), GRP (Glial-restricted precursor cells), astrocytes, neuroepithelial stem cells and oligodendrocytesCell number and death of O-2A/OPCs were analyzed by Calcein AM and PI Staining in live culture using a Celigo Cytometer.
Ferree, AndrewSeptember 2013MitoTimer Probe Reveals theImpact of Autophagy, Fusion and Motility on Subcellular Distribution of Youngand Old Mitochondrial Protein and on Relative Mitochondrial Protein AgeAutophagyMEF (Human Breast Cancer), COS (Monkey Kidney Tissue)To quanity red and green fluorescence intensity per cell in MEF and COS cells, cells were imaged using Celigo. Analysis parameters for images acquired by Celigo were optimized to identify individual MEF and COS cell based on fluorescence and the avg. green and red fluorescnce intensity for each cell was determined at the different time points.
Wang, YingAugust 2013Scalable Expansion of HumanInduced Pluripotent Stem Cells in the Defined Xeno-fress E8 Medium UnderAdherent and Suspension Culture ConditionsJournal of Stem Cell ResearchBC1 (human primary effusion lymphoma ), TNC1 (Rat type 1 astrocytes)The Total # of cell aggregates in a 1ml sample was analyzed by using the embryoid body analysis module in Celigo Imaging Cytometer. The avg. equivalent diameter of aggregates and the szie of each aggregate in the sample were also measured.
He, XianbaoAugust 2013The sst1 Resistance LocusRegulates Evasion of Type 1 Interferon Signalling by Chlamydia pneumoniae asa Disease Tolerance MechanismPLOS one: PathogensBone Marrow Derived MacrophagesT-cell survival Assay: Cells were plated and treated with infected C. pneumoniae and at designated time points were stained with Hoechst and PI. Total cell number and dead cell number were counted using Celigo Cytometer
Li, JianqingJune 2013A Short Hairpin RNA Screen ofInterferon-Stimulated Genes Identifies a Novel Negative Regulator of theCellular Antiviral ResponseMbio.Vero T144 (Kidney Epithelial Cells), NIH 3T3 (Human Fibroblasts), Hek293T (Human Embryonic Kideny cells), HeLa (Human Cervical Cancer)HeLa cells in 96-well plates were transfected with individual ISGs tagged with 3X Flag. After 24h, cells were infected w/WNV for another 24h and then fixed, permeabilized, and costained for WNV envelope protein (MAbE18)and the nucleus using DAPI. Images were captured and processed using a Celigo cytometer (Cyntellect). Infected and uninfected populations were gated separately, and infectivity was measured as the % of infected cells from the total cell counts.
Huang, HaiqingJune 2013iPSC-Derived B-Cells ModelDiabetes Due to Glucokinase DeficiencyJournal of Clincial InvestigationPatient Cell derived IPS to B-cell ModelQuantification of positively stained cells was performed using the celigo cytometer system.
Bian, ShuApril 2013P2X7 Integrates PI2k/AKT andAMPK-PRAS40-mTOR Signaling Pathways to Mediate Tumor Cell DeathPLOS oneMC138 (Colon Cancer Cell Line), B16/F10 (Melanoma cell line)Cells were seeded into Corning 3603 Black 96-well plates and grown for 24 hr before exposed to ATP for a short period of time. 16–24 hr later, cell growth was evaluated using the Celigo. BR images of live cells were captured using the Celigo Cell Counting application
Escribano-Diaz, CristinaMarch 2013A Cell Cycle Dependent RegulatoryCircuit Composed of 53BP1-RIF1 and BRCA1-CtlP Controls DNA Repair PathwayChoiceMolecular CellU2OS (human Osteosarcoma)U2OS cells were transfected with siRNA in 96-well plates. At 48 hr post-transfection cells were treated w/neocarzinostatin for 15 min. After a 3 hr recovery, cells were extracted with 0.2% Triton X-100 in PBS for 10 min on ice, fixed with 4% paraformaldehyde (PFA), and processed for RPA32 immofluorescence. Nuclei were counterstained with DAPI. Cells were imaged using the Celigo laser scanning plate cytometer (Brooks Automation) and analyzed with the accompanying image analysis software.
Vinci, MariaJanuary 2013Tumor Spheroid Based Migration Assays for Evaluation of Therapeutic AgentsMethods in Molecular Biology  
Lesovaya, EkaterinaJanuary 2013Combination of a SelectiveActivator of the Glucocorticoid Receptor Compound A with a Protease Inhibitoras a Novel Strategy for Chemotherapy of Hematologic MalignanciesCell CycleCEM (T- cell acute lymphoblastoma leukemia), K562 ( chronic myeloblastic leukemia), NCEB (mantle cell lymphoma) and multiple MM.1R (glucocorticoid resistant) and MM1S (glucocorticoid sensitive)The proliferation was measured by direct cell counting using Celigo Cytometer. Cells were plated at 10^4/well onto 24-well plates and cultured in complete medium in the presence of CdpA, Dex, Bortexomib or vehicle.
Signh, AnjaliJanuary 2013Profiliing Pathway SpecificNovel Therapeutics in Preclinical Assessment for CNS Atypical TeratoidRhabdoid TumorsMolecular OncologyBT12 and BT16 (CNS ATRT), KCCF1 (cerebral spinal fluid), Hs68 (Primary skin fibroblast), T98G [EGFR over-expressing glioblastoma multiform (GBM)]ATRT cells were trypsinized and placed in 96-well plates @ a concentration of 5x10^3 cells per well. Increasing concentrations of study agents were added to these wells to a final volu,e of 200ul per well. After 4 days, cell survival was quantified by Celigo Cytometer
Al-kasspooles, MazinJanuary 2013Preclincal Antitumor Activity ofa Nanoparticulate SN38Journal of Investigative New DrugsHT29, HCT116 (Human Colorectal Carcinoma) and H-meso-1 H226, H2052, MSTO-211H (Human Mesothelioma)Cells were plated in 96-well black clear bottom plates. After overnight incubation, PI was added and live and dead cell numbers at time 0 were determined using the Celiigo cytometer. Drug treatments were then added to cells . Live and dead cell numbers were analyzed again 24, 48, and 72 after drug addition.
Singh, LunJanuary 2013Profiling pathway-specific noveltherapeutics in preclinical assessment for central nervous system atypicalteratoid rhabdoid tumors (CNS ATRT): Favorable activity of targeting EGFR-ErbB2 signaling with lapatinibMolecular Oncologyatypical teratoid rhabdoid tumor (ATRT) cellsCell survival in 96-well plates was determined by Celigo in bright field after 4 days treatment with drug or control.
Stecklein,August 2012SI MethodsPNAS
Golipour, AzadehDecember 2012A Late Transition in SomaticCell Reprogramming Requires Regulators Distinct From the Pluripotency NetworkCell: Stem CellMEFsSecondary MEFs as indicated in the schematics were stained for AO and DAPI and then scanned with a Celigo hgih-content microscope to quantigy AP-positive areas. Dox withdrawals was quantified for 4 SC and 4 SI clones using the Celigo system. In both screens, 3 days after transfection, cells were fixed and stained for Dppa24, counterstained with DAPI and then subjected to automated analysis using the Celigo system for quantifying colony formation
Smith, KarenSeptember 2012Human Family with SequenceSimilarity 60 Member A (FAM60A) Protein: a New Subunit of the Sin3Deacetylase ComplexMolecular Cell ProteomicA549, HepG2 (Human Liver Cancer)A549 or HepG2 cells were transfected with control siRNAs or siRNAs against FAM60A. 3 days after transfection, cells were incubated for 5 hrs (A549) or 20hrs (HepG2) with BrdU. Cells were costained with DAPI. % incorporation of BrdU in each well was measured by fluorescence analysis using a Celigo Ceytometer
Stecklein, ShaneAugust 2012BRCA1 amd HSP90 Cooperate inHomologous and Non-Homologous DNA Double Strand Break Repair and G2/MCheckpoint ActivationPNASHCC1937 (Human Breast Cancer)DNA Synthesis, apoptosis and cell cycle distribution experiments in HCC1937 cells were performed on an LSRII flow cytometer or a Celigo adherent cell cytometer.
Nabzdyk, ChristophAugust 2012Differential Susceptibility ofHuman Primary Aortic and Coronary Artery Vascular Cells to RNA InterferenceJournal of Biochemical and Biophysical Research CommunicationsEndothelial (EC) and vascular smooth muscle (SMC) cellsCells were seeded at a density of 5000 cells/well of a 96-well plate. 24 hrs later cells were transfected with either non-targeting siRNA or non-targeting fluorescent red labelled siRNA using no transfection reagent., HiPerfect or Lipfectamine RNAiMax. Hoechst nuclei stain was used to label cells for counting. For data analysis an adherent cell cytometer Celigo was used.
McKevitt, I.April 2012SARS ORAL 2012British Journal Of Surgery Society Ltd.
Borrego-Diaz, EmmaApril 2012Overactivation of Ras SignalingPathway in CD133+ MPNST CellsJournal of NeurooncologyMCF7L (Human Breast Cancer)Functional analysis of cell growth and apoptosis was performed following knockdown of miRNAs using insitu cell cytometry (Celigo)
Healy, N.A.April 2012The Role of miRNAs in TamoxifenResistance in Breast CancerCellular and Molecular Life SciencesHuman MPNST primary cells and mouse MPNSTs, S805, S462, T530, T532, SW10 (mouse Schwann cells) and HSCsThe spheres were counted after 40hr, 7 days and 10 days of incubation using both Celigo and a light microscope.
Hsieh, Jeng-LongMarch 2012Acquisition of an EnhancedAggressive Phenotypein Human Lung Cancer Cells Slected by Suboptimal Doses ofCisplatin Following Cell Deattachment and ReattachmentJournal of Cancer LettersA549, H1299, H1299-RI~H1299-R5 (Human Lung Cancer Cell Lines)To analyze cell proliferation 1000 cells were seeded in 96-well plates in the complete medium at 37C on day 0. The number of cells was counted daily from day 1 to day 4 using a Celigo cytometer according to themanufacturers instructions. The proliferation rate is expressed as a ratio of the numberof cells counted on days 2-4 by the number counted on day 1
Vinci, MariaMarch 2012Advances in Establishment andAnalysis of 3D Tumor Spheroid-based Functional Assays for Target Validationand Drug EvaluationBMC BiologyU-87 MG glioblastoma, SF188 glioblastoma, MDA-MB-231 (Human Breast Carcinoma),…37 other cancer cells lines.For rapid, routine imaging and analysis of tumor spheroids, we utilized a Celigo cytometer, which is a bench top in situ celluar analysis system providing high quality, ful or partial images of wells using BR or FL illumination.
Huang, Han-HungFebruary 2012A Replacement for IsletEquivalents with Improved Reliability and ValidityActa DiabetologicaMurine Pancreatic Islet cellsIsolated islet cells were iamged and counted with Celigo Cytometer
Ponnurangam, SivapriyaFebruary 2012Honokiol in Combination withRadiation Targets Notch Signalling to Inhibit Colon Cancer Stem CellsMolecular Cancer TherapeuticsHCT-116 (Human Colon Carcinoma), SW480 (Human Adenocarcinoma of the colon)Colonosphere assay: The number and size of colonosphere were determined using Celigo
Schulz, RamonaJanuary 2012Inhibiting the HSP90 ChaparoneDestabilizes Macrophage Migration Inhibitory Factor and Thereby InhibitsBreast Tumor ProgressionJournal of Experimental MedicineU2SO (Osteosarcoma), SW480 (Human adenocarcinoma of the colon)Cell Confluence and cell numbers were evaluted over time by the Celigo Cytometer. Cell numbers (U2OS) or cell confluence (SW480) were measured using the Celigo cytometer using 49 squares per well
Gall, JonathanJanuary 2012Role of Mitofusin 2 in the RenalStress ResponsePLOS onePrimary Murine Kidney CellsCells were fixed in 4% paraformaldehyde for 10 min, washed with PBS and stained with PBS containing Hoechst for 30 min before being imaged and counted by Celigo cytometer.
Li, DanJanuary 2012The Role of CYP3A4 mRNATranscript with Shortened 3'-Untranslated Region in HepatocyteDifferentiation, Liver Development and Repsonse to Drug InductionMolecular PharmacologyHepaRG Cells (Human Hepatic Cells)The expression of RFP was analyzed in situ on an adherent cell cytometer to obtain cells images in both BR and red fuorescence, The ratios of cell densities from BR to red fluorescence in each entire well were analyzed by Celigo software.
Yoshida, ShunsukeNovember 2011Thrombospondin-2 Gene Silencingin Human Aortic Smooth Muscle Cells Improves Cell AttachmentJournal of the American College of SurgeryHAoSMC (Human Aortic Smooth Muscle Cell)Scratch assay (wound healing attchment assay): cell positions were determined using BR and Confluence modes of Celigo, and cell counting of cells stained with Hoechst was performed
Majumdar, IshitaNovember 2011Synthetic Cyclohexenyl ChalconeNatural Products Possess Cytotoxic ActivitiesAgainst Prostate Cancer Cellsand Inhibit Cysteine Cathepsins in VitroJournal of Biochemical and Biophysical Research CommunicationsDU-145 and PC3 (Human Colon Cancer) CellsApoptotic cells stained with Hoechst were quantitatively analyzed with the Celigo cyotmeter
Wang, Yen-ChaoNovember 2011Different Mechanisms forResistance to Trastuzumab Versus Lapatinib in HER2- Positive Breast Cancers,Role of Estrogen Receptor and HER2 ReactivationBreast Cancer ResearchBY474, UACC-812, AU-565, HCC-1569, HCC-1954, HCC-202, MDA-MB-361, MDA-MB-453, ZR75-30, SKBR-3, MCF7-HER2 (All Human Breast CancerCells were incubated with Annexin V-FITC and DAPI for 30 min and analyzed by the Celigo Cytometer for Apoptosis. Cells were stained with EdU and the proliferation rate was analyzed by the Celigo cytometer
Hoeksema, KimberlySeptember 2011Systematic in-Vitro Evaluationof the NCI/NIH Developmental Therapeutics Program Approved Oncology Drug Setfor the Identification of a Candidate Drug Repertoire for MLL-RearrangedLeukemiaOncoTargets and TherapyKOPN8 (B-Cell Precursor Leukemia), SEM (Human Acute Lymphoblastic Leukemia), B1 (Human B-cells), MOLT-3 (Human T-Lymphoblast), TIB-202 (acute monocytic leukemia) and Patient Bone Marrow Stromal (BMS) cellsBright Field cell counting, viable cell numbers count and counting of primary leikemia samples.
Las, GuyAugust 2011Fatty Acids Suppress AutophagicTurnover in B-CellsJournal of Biological ChemistryINS1832/13 (Insulin-Producing ß-Cell Line)Images of cells stained with Hoescht and PI, in paper.
Wlodkowic, DonaldMay 2011Wormomtry-on-a-Chip: InnovativeTechnologies for in Situ Analysis of Small Multicellular OrganismsCytometry Part A.NoneMentioned as an example of an image cytometer
Lesovaya, E.A.May 2011Antitumor Effect of Non-stroidGlucocorticoid Receptor Ligand CpdA on Leukemia Cell Lines CEM and K562Biochemistry (Moscow)K562 (human myelogenous leukemia) cells, CEM (human lymphoblastic leukemia), transfected to create CEM-NF-_B-GFP-Luc, K562-NF-_B-
GFP-Luc, CEM-AP1-GFP-Luc, and K562-AP1-GFP-Luc
Direct Count of suspension cells and a good cell count curve as an in paper figure
Nabzdyk, ChristophApril 2011High-Throughput RNAi AssayOptimization Using Adherent Cell CytometryJournal of Translational MedicineHuman Aortic Smooth Muscle CellsCells were stained with Cell Tracker Green (Invitrogen) and with Hoechst nuclei stain. Plates were read using the adherent cell cytometer equipped with a brightfield and three fluorescent channels: a blue filter for the Hoechst, red filter for the siGLO Red, and green for the Cell Tracker Green cytoplasmic dye.
Tingen, CandaceMarch 2011A Macrophage and ThecaCell-Enriched Stromal Cell Population Influences Growth and Survival ofImmature Murine Follicles in VitroSociety for Reproduction and FertilityLive, adhered ovarian stroma cells (Mouse)Imaging and cell counts taken from cells stained with Hoechst and FITC
Feng, LiliMarch 2011Vascular CD39/ENTPD1 DirectlyPromotes Tumor Cell Growth by Scavenging Extracellular AdenosineTriphosphateNeoplasiaU251 (Human Neuronal Glioblastoma), MDA-MB-231 (Human Breast Adenocarcinoma), A431 (Human Epidermal Carcinoma) and 5637 (Human Bladder Carcinoma)Cell confluence was determined regularly for 16 days using the Celigo Cell Cytometer
Bug, MMarch 2011Anthracyclines induce theAcculumation of Mutant p53 Through E2F1-Dependant and Independent MechanismsOncogeneLuciferase-expressing B16/F10, C57BL/6 (mouse melanoma) and Syngeneic C57BL/6murineMCA38 (colon cancer)Cell growth of cells exposed to ATP was evaluated with images and cell counting using the Celigo counter. Images present in paper
Kaestner, PhillipFebruary 2011Therapeutic Targeting of theMitotic Spindle Checkpoint Through Nanoparticle-Mediated siRNA DeliveryInhibits Tumor Growth in VivoJournal of Cancer LettersHCT116 Cells (Human Colon Carcinoma)Read cell numbers at 24hr increments
Wlodkowic, DonaldJuly 2010Cytometry in Cell NecrobiologyRevisited. Recent Advances and New VistasCytometry Part A.NoneOnly mentioned as a comparable cytometric thenologu
Sirmaci, AsliMay 2010A Truncating Mutation inSERPINB6 is Associated with Autosomal Recessive Nonsyndromic SensorineuralHearing LossThe American Jounal of Human GeneticsHeLa cells, (Human Cervical Cancer) Transfected with SERPINB6-WT-GFP and SERPINB6-MUT-GFP3-Channel, 8-bit stitched images were generated covering whole wells to identify the surface area & # of cells along with fluorescent intensities.
Hart, C.P.May 2010Product Focus: High-ContentScreening and Imaging: Instrumentation, Analysis and ApplicationsJournal of Biomolecular ScreeningNoneProduct focus, describeds product capabilities

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