Nexcelom Bioscience


Fluorescent Assay Protocols & Demonstrations
View all Fluorescent applications →

Based on demonstrations we have conducted, as well as work we have done with current customers, we have put together three different types of documents.

Assay Overview

These documents provide a quick snapshot of how an assay could be performed on the Celigo, with some images and results of the assay. Download the complete Celigo Assay Overview eBook for all Assay Overviews.

Demonstration Experiment

These documents are created from actual experiments that have been conducted on the Celigo. The documents review how the experiment was done using the Celigo, and summarizes the results from the experiment. Download the complete Celigo Demonstration Experiment eBook for all Demonstration Experiments.

Application Protocol

These documents are instructional, and provide step-by-step details for customers to follow if they are running the assay on their Celigo, or for potential customers to review if they are setting up the experiments they will be conducting during the Celigo demonstration. For a copy of an Application Protocol document you see listed available here, please reach out to your local Applications Scientist, email us at or call 978-327-5340

Title Assay Overview


Demo Experiment


Application Protocol
Cell cycle analysis using DAPI and BrdU  
Cell viability analysis using calcein AM, Hoechst and PI  
Endpoint apoptosis using Caspase 3/7 with Hoechst
Cell cycle analysis using EdU and DAPI    
Endpoint viability using DRAQ7 and Hoechst
Kinetic viability using DRAQ7
High-throughput suspension cell count and viability using AO/PI
High-throughput suspension cell count and viability using AO/PI and BF    
p53 and phosphor-p53 fluorescent marker analysis  
Endpoint viability using Propidium Iodide and Hoechst
Kinetic viability using PI
Kinetic apoptosis using Caspase 3/7
Cell Cycle PI Analysis using A375 cells    
HPC proliferation measurement using Ki-67 cellular marker
Antibody-Dependent Receptor Internalization Assay    
Count and measure cell infectivity of microcarriers    
Antibody Cell Surface Binding Detection  
GFP Transfection Efficiency Measurement    
Antibody-Dependent Drug Uptake Assay    
Kinetic pHrodo Antibody Internalization using Confluence  
Kinetic antibody internalization using pHrodo and bright field  
Endpoint antibody internalization using pHrodo and Hoechst