Nexcelom Bioscience

978-327-5340

Allows the User to Image Every Cell in Every Well on the Plate

  1. Best in-class imaging capability for suspension and adherent cells
  2. Generate whole well images for 6, 12, 24, 48, 96, 384, 1536-well plates
  3. Visually monitor labeled tumor target cells and the formation immune complexes over time

Bright Field and Four Fluorescent Channels for Cell Imaging and Analysis

Celigo® is a bench-top, micro-well plate-based, bright field and fluorescent imaging system. The best-in-class bright field imaging capability, in combination with 4 fluorescence channels, provides high speed, fully automated imaging and quantification of suspension and adherent cells in 6, 12, 24, 48, 96, 384, 1536-well plates.

Using Celigo image cytometer has significant benefits over traditional epi-fluorescent and bright field microscopy. Visualize and capture whole well images for an entire plate, perform automated cell counting and population analysis on a per well basis and finally significantly increase throughput by imaging a whole 96-well plate in less than 5 minutes.

Celigo Image Cytometer Captures Whole Well Images

tumor t-cells whole well image

By acquiring high resolution whole well images, we are able to image every cell in every well. Tumor target cells are stained with calcein AM.

•	Whole well image of GFP/RFP transfected HeLa cells from a 96-well plate

Whole well image of GFP/RFP transfected HeLa cells from a 96-well plate


proprietary optics

Proprietary optics and scanning system enable fast imaging of entire well, while maintaining consistent illumination and contrast out to the well edge, for accurate identification of all cells within each well.


Adherent Cells

adherent cells

Suspension Cells

suspension cells

The Celigo can image both suspension and adherent cells in both bright field and fluorescent modes.

E:T Ratio-Dependent NK Cell-Mediated Cytotoxicity
(Stained K562 Cells Imaged at t = 4 hr)

K562 cells stained with Calcein AM

K562 cells stained with calcein AM are killed by effector cells in an E:T ratio-dependent manner.

 

Time-Dependent NK Cell-Mediated Cytotoxicity
(NK to K562 Cell Ratio (E:T) = 2.5:1 )

killing of calcein AM stained K562 cells by effector cells

By imaging the sample plate over a period of 4 hours, we can observe the killing of calcein AM stained K562 cells by effector cells.

Time-Dependent NK Cell-Mediated ADCC Images

Time

Time-dependent NK ADCC

The number of calcein AM positive target cells decreased as the incubation time increased with NK cells. The morphology of NK cells and target cells are visually different and it also showed that the NK cells cluster around the target cells to induce cytotoxicity.

Fluorescent Images of Cellular Cytotoxicity at Different E:T Ratios

Calcein-AM stained target cells

Imaged calcein AM stained target cells with different concentrations of effector cells. The number of live, calcein AM positive cells decreases as the number of effector cells is increased.

Counted fluorescent images of cellular cytotoxicity

Counted fluorescent images of cellular cytotoxicity at different E:T Ratios

Visualize the Formation of Immune-Complexes Over Time

Time

immune-complexes over time

As time increased, the number of live target cells decreased.


Antibody Concentration

Antibody concentration over time

As antibody concentration increased, the number of live target cells decreased. Additionally, with an increasing amount of antibodies the number of immune cell clusters also increases.

 
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