Nexcelom Bioscience

978-327-5340

Applications for Cellometer Auto 1000 Bright Field Cell Counter

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Performance of the Cellometer Auto 1000 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Auto 1000
Average 1.03E+06 1.05E+06
STDEV 6.60E+04 7.76E+04
%CV 6.4% 7.4%

5 µm beads

N = 20 Hemacytometer Auto 1000
Average 1.07E+06 1.15E+06
STDEV 5.90E+04 6.35E+04
%CV 5.5% 5.5%