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Prepare Sample
Assay #1: Mix trypan blue with cell sample for viability & live cells.Assay #2: Directly measure highly viable sample w/o trypan blue for total cells.
Cellometer Cell Counting Chamber
With precision optical properties for imaging.With consistent volume of liquid measured.
Pipette 20 µl cell sample into a counting chamber. Spread cells evenly inside.
Capture Cell Images
Brightfield cell images are used for trypan blue.
Cellometer captures cell images from multiple locations.
Cellometer Image Analysis Software
Automatic analysis of cell images to identify:
• Live / dead cells
• Exclude sample debris
• De-cluster cell clumps for better accuracy
• Cell size
• Irregular cell shapes
Presenting Cell Counting Data
Cell Image
Counted Cell Image
Save cell images.
View counted cells.
Cell concentration and viability.
Cell size histogram.
Adjust cell sample after counting
Adjust cell sample for desired concentration by entering the target concentration in the calculator. The software will determine automatically for the amount diluents to add or to spin down for less liquid. Input desired number of cells, the calculator will output amount of liquid to pipette.
Export data
Cell counting data is saved in a table. The data table can be printed, or export to Excel for further calculation.
Cellometer Videos
Cell Counting Essentials
More About Cellometer Auto
Nexcelom Bioscience LLC. | 360 Merrimack Street, Building 9 | Lawrence, MA 01843
Telephone: 978.327.5340 | Fax: 978.327.5341 | Email: info@nexcelom.com | www.nexcelom.com
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