Cellometer Auto X4 Applications
Yeast Concentration & Viability
Live yeast cell concentration and viability are measured automatically based on the membrane permeability of the fluorescent dye Oxonol. Multiple strains of yeast with viability ranging from 88 to 5% were measured. »
Cellometer Auto X4 (10x) for Yeast Concentration & Viability
Yeast concentration and viability determination is essential in a number of different fields, including life science
research and the brewing industry. The Auto X4 (10x) Cell Counter offers several key advantages over manual yeast
counting and other automated counters:
- 1-step determination of yeast concentration & viability
- >75% reduction in test time vs. manual counting
- Automated data capture eliminates manual errors and inter-operator differences
- On-screen cell images for visual verification
- Pattern-recognition software for accurate analysis of cell clumps
- Mean cell diameter and distribution data
- No washing or maintenance
- Results in < 60 seconds
- 85% lower per test cost than competing automated yeast counter
Cellometer Auto X4 (10x) for Brewing
Monitoring of yeast viability during the fermentation process is an important quality control procedure employed by the brewing industry. Consistent yeast viability during the fermentation process is critical for batch-to-batch consistency and overall product quality. The Cellometer Auto X4 (10x) Fluorescent Cell Counter is specifically optimized for simple, 1-step determination of yeast concentration and viability and is ideal for both small and large breweries looking to automate their fermentation monitoring. Performance of the Auto X4 (10x) has been proven in the largest breweries in the U.S.
Brightfield and Fluorescent Imaging
Brightfield images can be viewed to confirm cell morphology. Counted brightfield images show counted cells within cell clumps. Fluorescent images show dead cells for confirmation of viability results. Analysis of both brightfield and fluorescent images from each sample makes 1-step concentration & viability determination possible.
Nucleated Cell Concentration
The Auto X4 Fluorescent Cell Counter utilizes fluorescence detection and staining with AO (acridine orange) to identify
nucleated cells from heterogeneous samples, such as unlysed whole blood, cord blood, and BAL (bronchoalveolar lavage)
fluid, as well as counting of PBMCs, splenocytes and other primary cells.
BAL
Cord Blood
Human WBC in Unlysed Whole Blood
Human PBMC
Mouse Spleenocyte
| Sample Name | Cell Count | Dilution Factor | Concentration |
|---|---|---|---|
| PBMC+ AO | 308 |
2 |
1.07E+06 |
| Human cord blood | 806 |
2 |
2.80E+06 |
| Human WBC+ AO | 220 |
10 |
3.85E+06 |
Live Cell Concentration & Fluorescent Viability Determination for Cell Lines & Clean Samples
The Auto X4 utilizes single fluorescence with a variety of different stains to easily and accurately count dead (non-viable)
cells. Brighfield images are used to generate total cell counts. Cell concentration and % cell viability are automatically
calculated based on sample volume and live / dead cell counts.
(For viability determination in messy samples or samples containing debris, dual fluorescence is required. See the Cellometer Auto 2000 Cell Counter and the Cellometer Vision Cell Analyzer.)
(For viability determination in messy samples or samples containing debris, dual fluorescence is required. See the Cellometer Auto 2000 Cell Counter and the Cellometer Vision Cell Analyzer.)
Live Cell Concentration and PI Viability
SKBr3
Cell Stained with DAPI
SKBr3
Cell Stained with Hoechst
SKBr3
Cell Concentration and Viability by Fluorescein Diacetate(FDA) Hydrolysis
T47D
Basic Cell Concentration & Trypan Blue Viability
Like the Auto T4 Cell Counter, the Auto X4 Fluorescent Cell Counter can also perform basic cell concentration and
trypan blue viability determinations using brightfield imaging. This method is widely used for most cell lines.
293
CHO
L929
Mouse RBC
Neuroblastoma
PEC
| Sample Name | Cell Count | Dilution Factor | Concentration | Mean Diameter |
|---|---|---|---|---|
| 293 | 372 |
1 |
6.65E+05 |
17.5 |
| CHO | 436 |
1 |
7.65E+05 |
11.5 |
| L929 | 542 |
1 |
9.58E+05 |
13.8 |
| Mouse RBC | 3158 |
1 |
5.46E+06 |
6.7 |
| Neuroblastoma | 103 |
1 |
1.82E+05 |
13.8 |
| PEC | 942 |
1 |
1.63E+06 |
7.4 |
293 Trypan Blue
Hela Trypan Blue
| Sample Name | Count Type | Cell Count | Dilution Factor | Concentration | Viability |
|---|---|---|---|---|---|
| Cultured stem cells+trypan Cultured stem cells+trypan |
Live Trypan |
445 30 |
2 2 |
1.57E+06 1.06E+05 |
93.70% |
| HeLa+trypan HeLa+trypan |
Live Trypan |
209 438 |
2 2 |
7.37E+05 1.55E+06 |
32.30% |
| HL60+trypan blue HL60+trypan blue |
Live Trypan |
160 19 |
2 2 |
5.65E+05 6.71E+04 |
89.40% |
Cellometer Videos
Cell Counting Tutorials
More About Auto X4
