Nexcelom Bioscience

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Cellometer Auto X4 Applications: Cellometer automated cell counters are pre-configured for more than 250 cell types

Cellometer Auto X4 Applications

Platelets

Platelets

Automatically measure mouse and human platelet concentration using brightfield only method. »

Yeast Viability

Yeast Concentration & Viability

Live yeast cell concentration and viability are measured automatically based on the membrane permeability of the fluorescent dye Oxonol. Multiple strains of yeast with viability ranging from 88 to 5% were measured. »

Cellometer Auto X4 (10x) for Yeast Concentration & Viability

Yeast concentration and viability determination is essential in a number of different fields, including life science research and the brewing industry. The Auto X4 (10x) Cell Counter offers several key advantages over manual yeast counting and other automated counters:
  • 1-step determination of yeast concentration & viability
  • >75% reduction in test time vs. manual counting
  • Automated data capture eliminates manual errors and inter-operator differences
  • On-screen cell images for visual verification
  • Pattern-recognition software for accurate analysis of cell clumps
  • Mean cell diameter and distribution data
  • No washing or maintenance
  • Results in < 60 seconds
  • 85% lower per test cost than competing automated yeast counter
Yeast cell image

Cellometer Auto X4 (10x) for Brewing

Monitoring of yeast viability during the fermentation process is an important quality control procedure employed by the brewing industry. Consistent yeast viability during the fermentation process is critical for batch-to-batch consistency and overall product quality. The Cellometer Auto X4 (10x) Fluorescent Cell Counter is specifically optimized for simple, 1-step determination of yeast concentration and viability and is ideal for both small and large breweries looking to automate their fermentation monitoring. Performance of the Auto X4 (10x) has been proven in the largest breweries in the U.S.

Yeast cell images

Brightfield and Fluorescent Imaging

Brightfield images can be viewed to confirm cell morphology. Counted brightfield images show counted cells within cell clumps. Fluorescent images show dead cells for confirmation of viability results. Analysis of both brightfield and fluorescent images from each sample makes 1-step concentration & viability determination possible.

Nucleated Cell Concentration

The Auto X4 Fluorescent Cell Counter utilizes fluorescence detection and staining with AO (acridine orange) to identify nucleated cells from heterogeneous samples, such as unlysed whole blood, cord blood, and BAL (bronchoalveolar lavage) fluid, as well as counting of PBMCs, splenocytes and other primary cells.

BAL

BAL (bronchoalveolar lavage) BAL (bronchoalveolar lavage) fluorescence
BAL (bronchoalveolar lavage) fluorescence counted

Cord Blood

Cord blood Cord blood fluorescence
Cord blood fluorescence counted

Human WBC in Unlysed Whole Blood

Human white blood cells in unlysed whole blood Human white blood cells in unlysed whole blood
Human white blood cells in unlysed whole blood

Human PBMC

Human PBMC Human PBMC fluorescence
Human PBMC fluorescence counted

Mouse Spleenocyte

Mouse Spleenocyte Mouse Spleenocyte fluorescence
Mouse Spleenocyte fluorescence counted
Sample Name Cell Count Dilution Factor Concentration
PBMC+ AO
308
2
1.07E+06
Human cord blood
806
2
2.80E+06
Human WBC+ AO
220
10
3.85E+06

Live Cell Concentration & Fluorescent Viability Determination for Cell Lines & Clean Samples

The Auto X4 utilizes single fluorescence with a variety of different stains to easily and accurately count dead (non-viable) cells. Brighfield images are used to generate total cell counts. Cell concentration and % cell viability are automatically calculated based on sample volume and live / dead cell counts.

(For viability determination in messy samples or samples containing debris, dual fluorescence is required. See the Cellometer Auto 2000 Cell Counter and the Cellometer Vision Cell Analyzer.)

Live Cell Concentration and PI Viability

SKBr3

SKBr3 cells SKBr3 cells counted
SKBr3 cells fluorescence SKBr3 cells counted fluorescence

Cell Stained with DAPI

SKBr3

SKBr3 cells stained with DAPI SKBr3 cells stained with DAPI fluorescence

Cell Stained with Hoechst

SKBr3

SKBr3 Horeschet SKBr3 Horeschet

Cell Concentration and Viability by Fluorescein Diacetate(FDA) Hydrolysis

T47D

T47D cells T47D counted cells
T47D cells Fluorescein Diacetate(FDA) Hydrolysis T47D counted cells Fluorescein Diacetate(FDA) Hydrolysis

Basic Cell Concentration & Trypan Blue Viability

Like the Auto T4 Cell Counter, the Auto X4 Fluorescent Cell Counter can also perform basic cell concentration and trypan blue viability determinations using brightfield imaging. This method is widely used for most cell lines.

293

293 cells 293 cells counted

CHO

CHO cells CHO cells counted

L929

L929 cells L929 cells counted

Mouse RBC

Mouse red blood cells Mouse red blood cells counted

Neuroblastoma

Neuroblastoma Neuroblastoma cunted

PEC

PEC cells PEC cells counted
Sample Name Cell Count Dilution Factor Concentration Mean Diameter
293
372
1
6.65E+05
17.5
CHO
436
1
7.65E+05
11.5
L929
542
1
9.58E+05
13.8
Mouse RBC
3158
1
5.46E+06
6.7
Neuroblastoma
103
1
1.82E+05
13.8
PEC
942
1
1.63E+06
7.4

293 Trypan Blue

293 cells stained with trypan blue 293 cells counted stained with trypan blue

Hela Trypan Blue

Hela cells stained with trypan blue Hela cells counted stained with trypan blue
Sample Name Count Type Cell Count Dilution Factor Concentration Viability
Cultured stem cells+trypan
Cultured stem cells+trypan
Live
Trypan
445
30
2
2
1.57E+06
1.06E+05
93.70%
HeLa+trypan
HeLa+trypan
Live
Trypan
209
438
2
2
7.37E+05
1.55E+06
32.30%
HL60+trypan blue
HL60+trypan blue
Live
Trypan
160
19
2
2
5.65E+05
6.71E+04
89.40%