Nexcelom Bioscience

978-327-5340

Applications for Cellometer K2 Fluorescent Viability Cell Counter

Adipocytes

Adipocytes

Automatically measure cell size of freshly isolated adipocytes and plot size histogram. DNA staining fluorescence dyes are used to identify cells from lipid droplets. »

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Apoptosis

Apoptosis

Automatically detect and analyze Caspase3 and 8, JC-1, and Annexin V apoptotic events using the Cellometer image cytometery. »

Cell Cycle

Image Cytometry for Cell Cycle Analysis

Automatically measure the cell cycle of mammalian cells. Generated cell cycle histogram allows for easy data analysis and presentation. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Viability and Necrosis

Cell Viability and Necrosis

Cell viability is performed using various fluorescent membrane exclusion dyes, such as PI, EB, 7AAD, and others. This assay is performed by enumerating cells in captured bright-field and fluorescent images. And Necrotic cells are detected using propidium iodide. »

Blood Samples

TNC Concentration & Viability for Clinical (Blood) Samples

Analyze fresh and processed blood and bone marrow samples without lysing: no interference from RBCs. »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

GFP Transfection Efficiency

Quantitative Measurement of GFP Transfection

Rapidly identify fluorescence positive cells from a sample, analyze individual cell fluorescence intensity, calculate cell concentration, size and determine the GFP transfection automatically. »

Hepatocytes

Fresh & Cryo Preserved Primary Hepatocytes

Automatically measure live hepatocyte concentration and viability using dual fluorescent nuclear stains, for human, rat, mouse and horse. »

Immunology

Immunology Research

Automatically quantify cell viability and concentration for a variety of immunologically relevant samples such as: bone marrow, cord blood, slpenocytes, lymphocytes, isolated mononuclear cells, tumor digests, murine samples, and others. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

PBMC

Peripheral Blood Mononuclear Cells (PBMC)

Automatically measure live cell concentration and viability without lysing red blood cells for consistent results from patient samples. Other cells include splenocytes and bone marrow. »

WBSc in Whole Blood

WBCs in Whole Blood

Automatically measure nucleated cell concentration without lysing red blood cells using nuclear staining dyes (AO), for human and mouse blood. »

Performance of the Cellometer K2 Image Cytometer

Total Cell Concentration Range of Jurkat Cells Measured by Cellometer K2

Dynamic Range of Total Cells with K2

Figure 1: Table of results for cell concentration dynamic range

Concentration Dynamic Range Figure 1 depicts the dynamic range for cell concentration measurements on Cellometer K2. This data set was taken on a concentration series of cultured Jurkat cell line.

Samples from 1 x 105 – 1 x 107 cells/ml can be counted without further dilution.

The %CV at each concentration was below 10%.

Cellometer K2 Repeatability and Consistency

Sample N Value Average Live Cell Concentration % Viability CV of Concentration CV of Viability

Jurkat

24

3.61E+06

92.2%

8.9%

1.0%

Human PBMC

10

5.94E+06

96.0%

4.7%

0.5%

Mouse Splenocyte

10

1.86E+07

88.6%

5.6%

0.7%

Figure 2: Table of results for cell concentration and viability using AOPI

The results indicate the accuracy of the Cellometer K2 instrument in assessing the viability of Jurkat cells using AOPI for cell viability. Jurkat, human PBMC, mouse splenocytes were tested at 24, 10, and 10 sample replications, respectively. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer K2 in measuring cell concentration and viability of mammalian cells.

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer K2
Average 1.03E+06 1.04E+06
STDEV 6.60E+04 5.57E+04
%CV 6.4% 5.3%

5 µm beads

N = 20 Hemacytometer K2
Average 1.07E+06 1.03E+06
STDEV 5.90E+04 5.38E+04
%CV 5.5% 5.2%