Prepare Sample

Switched between bright field, fluorescent channel 1 &2 are automatic using software.

Multiple assays methods can be used by different users.




Assay #1: mix AO / EB with cell sample for live / dead nucleated primary cells

Assay #2: mix PI with GFP transfected cells for viability and Transfection efficiency

Assay #3: mix Annexin-V conjugated with FITC and PI for apopotosis and necrosis

Assay #4: mix AO with cell sample for nucleated primary cells

Assay #5: mix PI with cell sample for PI viability & live cell concentration

Assay #6: mix trypan blue with cell sample for viability & live cell concentration

Assay #7: directly measure highly viable sample w/o trypan blue for total cell concentration

Cellometer Automatic Cell Counting Chamber

With precision optical properties for imaging.

With consistent volume of liquid measured.

Pipette 20 µl cell sample into a counting chamber. Spread cells evenly inside.

Cellometer Vision Demo

This video shows a demonstration of measuring live cell concentration and viability of human PBMC using Cellometer Vision.

Capture Cell Images

Brightfield and fluorescent cell images are both taken for primary samples or fluorescent viability assays.

Only brightfield cell images are used for trypan blue assay.

Cellometer captures cell images from multiple locations.

Cellometer Image Analysis Software

Automatic analysis of cell images to identify:

• Live / dead cells
• Fluorescent cells in color 1, color 2 or both colors
• Fluorescent intensity
• Exclude sample debris
• De-cluster cell clumps for better accuracy
• Cell size
• Irregular cell shapes

Presenting cell counting data

Save cell images.
View counted cells.
Cell concentration and viability.
Cell size histogram.

Adjust cell sample after counting

Adjust cell sample for desired concentration by entering the target concentration in the calculator. The software will determine automatically for the amount diluents to add or to spin down for less liquid. Input desired number of cells, the calculator will output amount of liquid to pipette.

Export data

Cell counting data is saved in a table. The data table can be printed, or export to Excel for further calculation.

Download:
Vision CBA Spec Sheet (PDF)