Nexcelom Bioscience

978-327-5340

Juno Therapeutics CAR-T Cell Therapy using Cellometer K2

cellometer | April 24, 2018 | No Comments | Cellometer Application News

A beautiful video from Juno Therapeutics, Inc. about the very human side of CAR-T cell therapies. We at Nexcelom are honored to see our Cellometer K2 featured here and play a part in this amazing work. 

Select Science interviews Founder and CTO of Nexcelom

cellometer | April 19, 2018 | No Comments | General Discussions

Great interview with our Founder and CTO, Jean Qiu, about Nexcelom and specifically the Celigo imaging cytometer.

Screen Shot 2018-04-17 at 8.36.56 AM

Automating Cell Imaging and Counting with Celigo

Dr. Jean Qiu, founder and Chief Technology Officer at Nexcelom Bioscience LLC, discusses the new automated imaging and cell counting platform: Celigo. Celigo is an innovative, high-throughput system which enables integrated image and data acquisition from a variety of microplates. High-speed whole well image acquisition coupled with a bright field and 4 fluorescent channels enables Celigo to perform a multitude of 2D and 3D assays.

Poster: High-throughput immune complex analysis method for CAR T cell-mediated cytotoxicity using the Celigo Image Cytometer

cellometer | April 5, 2018 | No Comments | Celigo Application News

car-t-poster

Download our Poster

Abstract: Cancer immunotherapy has been gaining momentum in the field of cancer research. Specifically, Chimeric Antigen Receptor (CAR) T cell technology have introduced new methods to combat cancer. Direct cell-mediated cytotoxicity assays are required to assess the killing capability of the engineered CAR T cells. Traditionally, these assays are conducted by measuring the amount of released Chromium, calcein AM, or LDH molecules after the target cancer cells are killed with CAR T cells. These methods require a large amount of target cells which may not be ideal when working with donor primary samples. Additionally, they cannot (more…)

Cellometer and Honey Bees

cellometer | March 15, 2018 | No Comments | Cellometer Application News

honey-bee

A study recently published in the Journal of Insect Behavior looks at the mating system of honey bees. Cellometer Vision CBA was used to count and determine the viability of collected honey bee sperm.

Check out the paper: Honey Bee Queens Do Not Count Mates to Assess their Mating Success

High throughput, high-resolution image acquisition for DNA damage detection

cellometer | March 2, 2018 | No Comments | Celigo Application News

Celigo playing an important role in high throughput, high-resolution image acquisition for DNA damage detection.

The Celigo S captures 16 images of each well that are then stitched into a single representation

The Celigo S captures 16 images of each well that are then stitched into a single representation

Open access article: Next generation high throughput DNA damage detection platform for genotoxic compound screening

Join Us in San Diego at SLAS2018

cellometer | January 25, 2018 | No Comments | Celigo Application News

Come see the Celigo Image Cytometer in action!

New software, reagents and automation.

Visit Booth #318, and discover how Imaging Cytometry can improve your lab’s workflow and increase its capabilities.

slas-announcement

The new Celigo Platform provides you with modular instrumentation and automation options, as well as, complementing reagent kits enabling you to run high-throughput cellular analysis for multiple applications including:

Cell line development, Virology, Immune-oncology, Cell counting, 3D spheroid and organoid analysis, Migration and invasion, iPSC reprograming, Fluorescent functional assays.

Come See Our Poster Presentations:

  • Real-time kinetic viability and apoptosis detection and screening of 3D multicellular tumor spheroids using the Image Cytometer
    Presented by: Olivier Déry, PhD., Nexcelom Bioscience
    Poster # 1046-B
    Monday, February 5 | 5:00 PM – 6:00 PM
  • A novel image cytometric analysis method for T cell-mediated cytotoxicity of 3D tumor spheroids
    Presented by: Olivier Déry, PhD., Nexcelom Bioscience
    Poster # 1099-E
    Wednesday, February 7 | 11:30 AM – 12:30 PM

The Science of Beer

cellometer | November 30, 2017 | No Comments | Cellometer Application News

Congratulation to Nexcelom’s Dr. Leo Chan for his Inge Russell Best Paper Award form @MasterBrewers . Dr. Chan’s paper, “Measuring Lager and Ale Yeast Viability and Vitality Using Fluorescence-Based Image Cytometry” examines the use of image cytometry for multichannel fluorescent assessment of yeast viability and vitality during the brewing process.

leo-mbaa-inge-russell-best-paper-award

Celigo Helping Move Research Forward to Find a Cure for Alzheimer’s

Dmitry Kuksin | November 7, 2017 | No Comments | Celigo User Publications

November is National Alzheimer’s Disease Awareness Month and research into a cure for the disease is vigorous. In a recently published paper “CRISPR/Cas9-Correctable mutation-related molecular andphysiological phenotypes in iPSC-derived Alzheimer’s PSEN2N141I neurons” researchers used the Celigo image cytometer upstream of CRISPR/Cas9 to visualize and asses cell death related to the sensitivity of iPSC-derived PSEN2N1411 neurons in the presence of Aβ42 oligomer toxicity.

Don’t let clumpy samples prevent you from getting an accurate count!

Aimee DesRoches | April 12, 2017 | No Comments | Cellometer Application News

Counting cells with a hemacytometer is a long, dull process on its own – but if you’re trying to count cells that are clumped together in your sample, it can become a real headache! Clumpy samples make it hard to discern the individual cells from one another, causing a high risk of human error and skewed cell counts. Your results can be extremely difficult to reproduce, as the cell count would likely vary from person to person counting the same clumpy sample, causing a lack of confidence in your data.  Luckily, we have a solution. The Cellometer Auto T4 automated cell counter is optimized for cultured cell lines, even the clumpy ones!

Cellometer Auto T4 counted cell image shows individual counted cells outlined in green.

Cellometer Auto T4 counted cell image shows individual counted cells outlined in green.

As the first automated cell counter ever manufactured, the Auto T4 set the standard with its pattern-recognition software and bright field imaging used to calculate cell count, concentration and viability with Trypan Blue.  If you are counting clumpy cell lines such as C6, HT29, DLD, KAT4, GHOST or MCF-7, just to name a few, you can use the Auto T4’s de-clustering settings to achieve an accurate cell count! It will identify and individually count each cell in your sample.

But don’t take our word for it – here’s what our customers have to say about the Auto T4:

“The Cellometer Auto T4 is a nice device for cell counting! The great feature is the ability to count clumps of cells!” – Tufts University

“The Cellometer Auto T4 automated cell counter makes the lab life fun and easy! Before this, I used a hemacytometer for counting cells which took so much time and had many difficulties, especially when the number of samples were large. The Auto T4 is able to ease that pain. Also, its precision is very high and numbers are very reproducible. Overall A+” – University of South Florida

“The Cellometer Auto T4 is a very high functioning machine that operates with high accuracy and precision for measuring concentrations and percent viability for cultured cells stained with Trypan Blue.  The Auto T4 meets the claims of reporting within 30 seconds the live, dead, and total cell count; the live and total cell concentration; the mean cell diameter; and the percent viability. I really love the ability and functions where it automatically generates a cell size histogram based on cell size. I love this machine and it is a must-have for our upstream lab!” – Southeast State University

Using the Auto T4 can quickly and accurately get you the results you need, using only 20uL of sample in less than 30 seconds!  Getting a precise cell count has never been so easy!  Eliminate human error with the hemacytometer and the struggle of counting clumps by letting the Auto T4 do the work for you!  If you are ready to automate your cell counting process, request a quote or speak with your local specialist today to learn more or request a free one week evaluation.

What do you need when you count cells manually with a microscope and hemacytometer?

Sara Barker | April 5, 2017 | No Comments | General Discussions

anna_microscope-neck-pain

…a neck massage!

There’s a new article out from Biocompare entitled “Cell Counts and More” – and we have to give joke credit to them. We think it’s a pretty good one, and accurate, just like our instruments. One of our customers, Tiffany Sidwell, of UCLA Immunogenetics Center, even provided some feedback on the very topic last year. Tiffany shared:

“Counting cells daily on the microscope was very bad for my occipital joints and muscles. Since using the Cellometer Auto 2000, I no longer have neck pain when counting cells. Thank you Nexcelom!”

There are many, many benefits associated with an automated cell counter: improved accuracy, reproducible results, efficient, health benefits… And depending on the automated cell counter you select, you can save assay parameters to use consistently, you can capture images and save results.

We think our automated cell counters are the best available, but we know there are a lot of options to select from, so as you are evaluating your options, our local Applications Scientists are here to answer questions you may have, set up a free in-lab demo, or work with you to arrange a free 1-week evaluation of most of our instruments.