Counting cells with a hemacytometer is a long, dull process on its own – but if you’re trying to count cells that are clumped together in your sample, it can become a real headache! Clumpy samples make it hard to discern the individual cells from one another, causing a high risk of human error and skewed cell counts. Your results can be extremely difficult to reproduce, as the cell count would likely vary from person to person counting the same clumpy sample, causing a lack of confidence in your data. Luckily, we have a solution. The Cellometer Auto T4 automated cell counter is optimized for cultured cell lines, even the clumpy ones!
As the first automated cell counter ever manufactured, the Auto T4 set the standard with its pattern-recognition software and bright field imaging used to calculate cell count, concentration and viability with Trypan Blue. If you are counting clumpy cell lines such as C6, HT29, DLD, KAT4, GHOST or MCF-7, just to name a few, you can use the Auto T4’s de-clustering settings to achieve an accurate cell count! It will identify and individually count each cell in your sample.
But don’t take our word for it – here’s what our customers have to say about the Auto T4:
“The Cellometer Auto T4 is a nice device for cell counting! The great feature is the ability to count clumps of cells!” – Tufts University
“The Cellometer Auto T4 automated cell counter makes the lab life fun and easy! Before this, I used a hemacytometer for counting cells which took so much time and had many difficulties, especially when the number of samples were large. The Auto T4 is able to ease that pain. Also, its precision is very high and numbers are very reproducible. Overall A+” – University of South Florida
“The Cellometer Auto T4 is a very high functioning machine that operates with high accuracy and precision for measuring concentrations and percent viability for cultured cells stained with Trypan Blue. The Auto T4 meets the claims of reporting within 30 seconds the live, dead, and total cell count; the live and total cell concentration; the mean cell diameter; and the percent viability. I really love the ability and functions where it automatically generates a cell size histogram based on cell size. I love this machine and it is a must-have for our upstream lab!” – Southeast State University
Using the Auto T4 can quickly and accurately get you the results you need, using only 20uL of sample in less than 30 seconds! Getting a precise cell count has never been so easy! Eliminate human error with the hemacytometer and the struggle of counting clumps by letting the Auto T4 do the work for you! If you are ready to automate your cell counting process, request a quote or speak with your local specialist today to learn more or request a free one week evaluation.
…a neck massage!
There’s a new article out from Biocompare entitled “Cell Counts and More” – and we have to give joke credit to them. We think it’s a pretty good one, and accurate, just like our instruments. One of our customers, Tiffany Sidwell, of UCLA Immunogenetics Center, even provided some feedback on the very topic last year. Tiffany shared:
“Counting cells daily on the microscope was very bad for my occipital joints and muscles. Since using the Cellometer Auto 2000, I no longer have neck pain when counting cells. Thank you Nexcelom!”
There are many, many benefits associated with an automated cell counter: improved accuracy, reproducible results, efficient, health benefits… And depending on the automated cell counter you select, you can save assay parameters to use consistently, you can capture images and save results.
We think our automated cell counters are the best available, but we know there are a lot of options to select from, so as you are evaluating your options, our local Applications Scientists are here to answer questions you may have, set up a free in-lab demo, or work with you to arrange a free 1-week evaluation of most of our instruments.
Based on an article published by NPR this week, the Craft Brewing Industry is alive and well, with over 5,000 craft brewers in operation last year. And for as many craft breweries that are out there, there are as many different ways to rank them! The Brewers Association will rank by sales and production; Vinepair has a map displaying the number of breweries by state; Thrillist seems to have organized the ranks based on their opinion of the offerings; Travel + Leisure positions the beer offerings with the city’s appeal.
Regardless of how you rank them, business is up. Take Tree House Brewing for example. In another NPR article, they were featured for attracting customers from as far as 6 hours away for their craft. They manage the demand by enforcing beer rations – requiring customers to fill out an order form, limiting each person to 10 cans and two 64-ounce growlers per visit.
Good indications for the Craft Brewing industry, in our opinion. And we support you, Craft Brewers. Come talk with us at the Craft Brewers Conference in Washington DC from April 11-13th at Booth# 4239.
STEM – Science, Technology, Engineering and Mathematics – is vital to our future. At present, our students are not stacking up with the other developed countries. We understand the value and importance of encouraging students to explore these STEM fields, and consider a career in a STEM.
For that reason, we created a package specifically designed with high schools, colleges and universities in mind. It’s called our Teaching Lab – and it has everything a teacher needs to bring automated cell counting technology into their classrooms and labs at a discounted price. The Cellometer Mini and Cellometer Auto 1000 are both options for this program – bright field automated cell counters. Incorporate one of these into a Research Initiatives or Research Methodology course, and have your students count cells for concentration and viability manually, with a hemacytometer, and then on the cell counter.
This introduces your students to the types of instruments and technologies they may encounter as progress in their science careers. Nexcelom’s Teaching Lab gives students a great hands-on experience with research tools and introduces them to automated cell counting methods, all while improving the competitiveness of your institution’s science and technology departments. If you’d like to know more about what is included in the package, check us out here.
Did your lab’s grant fall through? Funding not an option? We get it – money’s tight right now. In Nexcelom’s long line of automated cell counters, there is one instrument designed specifically with your budget in mind – the Cellometer Mini! As the lowest-cost cell counter on the market, it utilizes bright field imaging, and is perfect for any lab looking to achieve basic cell count, concentration and Trypan Blue viability of cultured cell lines and purified primary samples. It’s affordable, accurate, and small enough to fit in your purse or backpack! Need a little convincing? Don’t just take our word for it, here’s what our customers have to say:
“The Cellometer Mini is such a time saver compared to using the hemacytometer! We love it and use it all the time. It is easy for students and residents to use, which also saves us in training time.” – Southeast HSC
“The Cellometer Mini is incredibly rapid at counting cells. Even better, the software has a built-in calculator for determining dilutions. It speeds up our workflow.” – Baylor University
“The Cellometer is quick and convenient and I find the cell counts to be just as reliable as using a hemacytometer. My lab cultures several different cell lines and we save hours by substituting the Cellometer for the hemacytometer! I have trained my students in my cell lab to use the Cellometer and they now spend less time counting cells and have more time to do experiments!” – Wheeling Jesuit University
“I really appreciate the small footprint of the Cellometer Mini. It doesn’t take up much space and it is very easy to use. The guides provided make it a no-brainer!” – Leidos Biomedical Research
The Mini is user-friendly and fast, without sacrificing accuracy with its low cost. The instrument you want is obtainable on a budget! Contact us if you are interested in a quote, scheduling a free in-lab demo, or speaking with a specialist.
If you rely on a flow cytometer in a core lab or a central facilities lab, have you ever wondered if there was a better way? Have you ever wished to have full control over your experiments, rather than hand your sample over to a technician? There was an interesting article written recently about the needs of researchers. Have you seen this?
Those interviewed for the article suggest that there are some main factors for consideration:
- The instrument size needs a smaller footprint than existing products offer
- The price of the instrument should be under $100,000
- Software ease-of-use to reduce the need for dedicated technicians
- How many colors/markers are needed
If you’re open to an alternative to flow cytometry, we would suggest you consider image cytometry. It is what it sounds like. It performs assays and experiments like a flow, but there are no fluidics, just images. That means no clogging. No cleaning. No calibration. No maintenance. No headaches and no hassles!
Consider the Cellometer Vision CBA. The footprint is compact – 6″ wide x 8.5″ deep and 14″ high. The US price is less than half the standard budgets for a benchtop flow cytometer. The Cellometer software is designed to be intuitive, easy to use, and easy to customize; and if you aren’t fully confident, we have free support – both on-site and remote. The system is able to analyze 2 colors at a time, and with interchangeable filter optics modules, you have flexibility on the markers you use from experiment to experiment. If you need to sort your cells, this wouldn’t be your ideal instrument. But if you want to break free from the flow cytometer – request a quote or talk to a local scientist about setting up a free in-lab demo to see if for yourself.
If you’re using a microscope and hemacytometer to count your cells and determine viability, you might have felt like Sheldon, when he vacations in Amy’s lab and, among other things, helps out by counting the bacteria spores. Everything’s going well until Amy returns to the lab, distracts Sheldon, and then he has to start over with his count.
Fortunately for you, you don’t have to be like Sheldon if you have an automated cell counter. We have bright field counters for cultured cells and Trypan Blue exclusion assays. We have dual fluorescent cell counters for messy, primary samples that are great with a nuclear stain such as Acridine Orange (AO) and Propidium Iodide (PI). We also have image cytometry systems, giving you the power to perform cell based assays such as apoptosis, GFP/RFP Transfection, cell cycle, autophagy, mitochondrial potential, etc, with only 20 microliters of sample. All of our systems will take images and analyze the sample in less than a minute – with most systems completing in less than 30 seconds! Our systems can image and analyze cells as small as 0.5 microns up to as large as 300 microns in size!
If you are experiencing difficulty counting your PBMCs with Trypan Blue using a hemacytometer, we feel your pain! But did you know all that pain can be avoided? With the Cellometer Auto 2000 automated cell counter, counting peripheral blood mononuclear cells (PBMCs) and other complex primary samples is a breeze! Using dual fluorescence image-based cytometry and staining your samples with AO/PI (acridine orange and propidium iodide), the Cellometer Auto 2000 cell counter can detect live and dead nucleated cells while excluding debris, red blood cells, platelets in your sample. Here is what some of our happy customers counting PBMCs have to say:
“My colleague and I have purchased a Cellometer Auto 2000 cell counter. We are using it now and it has facilitated our work greatly. We routinely process PBMCs from both fresh whole blood and from frozen stock. The Cellometer has made it much easier to get cell numbers and viability percentages for use in downstream applications such as IVS and Elispot.” – Human Longevity, Inc.
“The Cellometer makes counting PBMC’s from serum really fast, so when I have to plate from 18 different samples it doesn’t take as long as a hemocytometer would!” – Colorado State University
“The Cellometer Auto 2000 makes cell counting much faster and more accurate than manual counting. Our laboratory needs to complete splenocyte and PBMC isolation protocols as quickly as possible, and the Cellometer allows us to count samples from 6 isolation batches in about 15 minutes. This gives us the ability to isolate more cells at a time, because we know they will not be sitting too long before we have a final count.” – Academic Research Laboratory
“Our Cellometer is an important asset to the work we do in our lab, specifically PBMC isolation. No common cell counter has the capacity to count PBMCs. We love the portability of the instrument (due to its small size) along with being able to see the cells on the screen and the extra features – such as sorting data, printout capability, and aliquot calculations. Truly a fantastic investment!” – Leading comprehensive cancer center lab
The Cellometer Auto 2000 can also be used for counting stem cells, splenocytes, irregular-shaped cells, messy and clean samples! Forget the hassle of red blood cell (RBC) contamination, broken hemacytometers, and inaccurate counts – as one of our fluorescent automated cell counters may be a great addition to your lab! We offer free in-lab evaluations of the Auto 2000, so if you’d like to try one for yourself – fill this form out!
Everyone at Nexcelom Bioscience is ready for the holidays this year! Several Nexcelom employees pitched in to assist with “decking the halls” – with a total of 3 different Christmas trees glowing throughout the office!
From everyone here at Nexcelom, wishing you and yours a very Happy Holidays and New Year!
-The Nexcelom Team
Antibody-directed and drug therapies have been on the forefront of immuno-oncology research in the past 15 years. Since the FDA introduced the Breakthrough Therapy Designation in 2016 for novel promising drug therapies, the number of applicants and approved candidates has increased dramatically.
As more and more researchers are looking at drug effects in cancer cells and moving forward in drug discovery, they face many challenges in their day to do work. Performing cell cytotoxicity assays for multiple cell lines with single and combination drug therapies is standard practice in immuno-oncology. While many researches use end-point assays such as MTT, XTT, WST, or CellTiter-Glo, others prefer the manual counting using Trypan Blue. While these methods may be popular, it does not mean they are without their limitations. By directly imaging and counting every cell in a well over a course of a drug treatment, the Celigo can perform the cytotoxicity assays in a label-free format.
We recently presented an informational webinar on this very topic. If you’re intrigued to learn what advantages and disadvantages we identified for these methods, we encourage you to watch the webinar. You can access it here.
Once you’ve had a chance to watch it, we’d love to hear your thoughts, opinions, and any questions you may have.