Cellometer® Automatic Cell Counters - Cell Counting Results

Method #1 Total cell concentration

Without special counting reagent or buffer, cells in growth media or PBS are loaded into Cellometer® disposable counting chamber slide. With one sample, the following data are produced by a single measurement.

Total cell concentration /ml
Mean Diameter
Total cell counts
Cell images
Cell counting data sheet
Cell size histogram

Verify counting results using 'Counted Cells' feature. Green circles indicate counted cells.

Method #2 Viable cell concentration and viability

Cell sample is mixed with trypan blue then loaded into a Cellometer® counting chamber. With one sample, the following data are produced by a single measurement typically within 30 seconds.

Live cell concentration /ml
Viability (% live cells to total cells)
Mean Diameter
Cell images
Cell counting data sheet

Counted cells' feature: green circles for live cells and red circles for dead cells.

Save cell counting data sheet

Date time	Cell type	Sample name	Total count	Mean diameter (mm)	Viability	Dilution factor	Concentration (cells/ml)
3/9/2006 15:37	Spleen_mouse	1	388	6.48	92.2	10	1.04E+07
3/9/2006 15:41	Spleen_mouse	2	401	6.38	86.8	10	1.08E+07
3/16/2006 13:31	Jurkat	J1-1	273	9.63	N/A	1	7.32E+05
3/16/2006 13:32	Jurkat	J1-2	283	9.53	N/A	1	7.59E+05
3/16/2006 13:33	Jurkat	J1-3	297	9.54	N/A	1	7.97E+05
3/16/2006 13:34	Jurkat	J2-1	276	9.48	N/A	1	7.40E+05
3/16/2006 13:34	Jurkat	J2-2	276	9.33	N/A	1	7.40E+05
3/16/2006 13:35	Jurkat	J2-3	275	9.37	N/A	1	7.38E+05
3/17/2006 13:22	PBMC_frozen	PBMC1	1092	7.59	97.3	20	5.86E+07
3/17/2006 13:24	PBMC _frozen	PBMC2	1094	8.01	96.6	20	5.87E+07

Save cell image

Cell images used for each cell counting can be saved for later re-count or for support. See Cell Image Gallery for more cell images.