Best Suited For:

• Total cell concentration for highly viable cell population
• Live cell concentration and viability using trypan blue
• Cell size measurement
• Identify and count cells based on cell size difference
• Capture and store cell images

1. Pipette 20µL Sample	2. Insert Slide	3. Get Data

The Cellometer Auto T4, a trypan blue viability cell counter, offers unique and superior functionalities and capabilities due to innovative design and sophisticated software. By using advanced cell counting chamber technology, Cellometer Auto T4 eliminates cross contamination, allows for no maintenance and immediate setup and use. This makes it suitable for occasional use or users with a larger volume of cell samples.

• High quality microscopic cell images for observation of cell morphology and sample quality
• Fast, accurate cell counting: less than 30 seconds for a typical cell line sample
• Declustering ability for clumpy cells
• Cell size histogram
• Review counted cells on screen for data verification
• Direct link to Nexcelom applications support team
• Auto saving for cell counting data and cell images for record keeping
• Ability to store and print data through computer network
• Sample adjustment calculation for the next step in the experiment to eliminate manual data transfer induced errors

Advanced features of Cellometer Auto T4 Include:

• Deep gap counting chamber with innovative counting method for cells or spheres larger than 100 micron
• Option to manually adjust cell count
• Use default cell parameter for simple cell counting
• Import cell types from Nexcelom cell library (250+ cell types)
• Cell type wizard for automatic set up new cell types
• Modify cell size parameters when needed and re-calculate for new results
• Auto sample ID increment for large numbers of samples
• Detailed data for each cell count saved in Excel for comparison between samples

	Compact, Simple to Use Cellometer Auto takes up almost no bench space and is incredibly easy to use. It's pre-configured for hundreds of cell types, and configuring for new cell types is just as easy. Because it works with your PC or laptop, you get a full screen display, easy data input, and the ability to import/export data to a local or network location. Cellometer also runs on Intel based Macs running Parallels/WinXP.
	Fast Results Because it can generate cell counts, size measurements, and concentration calculations in approximately 30 seconds, Cellometer enables you to generate data many times faster than manual counting.
	Minimal Sample Prep Disposable Counting Chambers is the only consumable needed to run Cellometer. Only 20uL of sample is required, and the chambers load in seconds, and when done can be disposed, eliminating the need for cover slips and washing between samples.
	Trypan Blue Viability Trypan Blue treated cells are automatically counted as live or dead, and the percentage viability is automatically calculated. Live counted cells are indicated by a green circle on-screen, and Dead counted cells by a red-circle.
	On-screen Imaging No two cells are the same, which is why live cell morphology can be immediately viewed on-screen, and counted cells are indicated on-screen for further analysis. The counting results are indicated for each cell and images can be saved or exported for a secure record of all of your cell counts.
	Cell Size Measurement Unlike manual counting, Cellometer measures both individual and mean cell sizes, and can generate size distribution histograms for all counted cells. Multiple histograms can be overlaid for determining changes in cell sizes over time.
	Advanced Imaging Technology Microscope quality optics and advanced vision software ensure that even the most challenging samples can be counted. This includes counting specific cells in a heterogeneous sample, declustering cell clumps (such as MCF7), counting irregular shaped cells, and excluding cellular debris.
	Save & Export Data, Images, and Graphs Keeping records of your research is critical. With the click of a button, Cellometer instantly saves counting results, cell images, graphs, and data to a clipboard, local or network location.
	Local, World Class Support Nexcelom support is provided from of our Boston area headquarters. Our support team includes PhD's and experts in cell biology, digital imaging systems, and software, and are available via phone or email to help setup, configure and use your Cellometer automated cell counter. We also offer online/onsite training & web based remote instrument control to assist with your cell counting applications.

Cellometer’s automatic cell counting principle is based on imaging cytometry. Cells in suspension are spread into a thin layer of liquid when loaded in a Disposable Counting Chamber. The instrument is connected to a computer via USB 2.0 connection and acquires cell images from multiple locations of the counting chamber. The software then analyzes these images and determines cell concentration, trypan viability and cell size. Results & cell images are displayed on-screen and in approximately 30 seconds.
	1. Load Counting Chamber Simply pipette 20uL into one side of a disposable counting chamber. It's nearly impossible to overload or underload, and capillary action automatically spreads your sample into a monolayer. Each slide holds two samples and feature a large, white writing surface for sample identification.
	2. Insert Silde The counting chamber is loaded into a slot on the front of the instrument. Click 'Display Image'', then 'Count' to begin counting.
	3. Get Data Less than 30 seconds later, counted images are displayed on-screen, as well as live cell count, dead cell count, concentration, and mean size. Cell size histograms and other data can be instantly displayed, and all data & images can then be saved, or exported.
Other Features
	Configuring for Different Cell Types Cellometer features more than 250 pre-programmed cell type parameters. New cell types are easily added via a New Cell Type Wizard or manual configuration. Configuring or fine tuning Cellometer for your specific assay needs is simple and straightforward, and our Support Team is always available to assist.
	Declustering Cellometer's advanced imaging can locate and accurately count cells in clumps or clusters. This feature is adjustable for your particular needs, and can also be turned off completely to count the number of clumps in a sample.
	Exclude Cellular Debris Because Cellometer recognizes cells based on size, brightness, and morphology- cellular debris can be excluded from counting results ensuring accurate results.
	Heterogeneous Cell Samples Minimum and maximum cell size parameters can be set to only count specific cells in a heterogeneous sample. This is deal for counting dendritic cells cultured from PBMC, or counting PBMCs amongst platelets.
	Irregular Cell Shapes Cellometer can recognize and count irregular shaped cells by adjusting the roundness cell setting. Ideal for counting RD cells or activated T-cells.
	Changes in Cell Diameter Because Cellometer generates individual cell size measurements, multiple samples can be overlaid on one histogram, enabling analysis of change in cell diameter over time, such as when counting SF9 or SF21 cells. Saved images can be viewed and counted later to analyze changes in morphology over time.

Example Cell Types

Immunology Applications

Cancer Cell Lines

Insect Cells

Heterogeneous Cell Samples

Yeast & Algae

Platelets

	Cellometer Auto T4 Plus:	Cellometer Auto M10:
Includes	Auto T4 Plus instrument, software, USB 2.0 connection cable, power supply & phone/online applications support during setup	Auto M10 instrument, software, USB 2.0 connection cable, power supply & phone/online applications support during setup
Cell Sizes	Most Cells 5-60 microns	2-12 microns Yeast, Platelets, & Algae
Concentration Range:	2.5x105/ml - 1.0x107/ml	5.0x105/ml - 5.0x107/ml
Performance Comparison data:	Total Count vs. Hemacytometer Viability vs. Hemacytometer PBMC Dilution Series L929 Dilution series	Contact Us for more information.
Weight: Dimensions: Power Input:	10 lbs W5.0" x D5.0" x H13.0" 100-240V AC, 50-60Hz, 0.5A
PC or Laptop requirements:	Windows XP or Vista 2.4GHz or higher 512MB Memory USB 2.0 Port -- Intel Mac with Parallels/WInXP
Contact us for additional information.