Best Suited For:

• Total cell concentration for highly viable cell population
• Live cell concentration and viability using trypan blue
• Cell size measurement
• Identify and count cells based on cell size difference
• Capture and store cell images
• Total nucleated cell concentration without lysing non-nucleated cells
• Live nucleated cell concentration without lysing non-nucleated cells
• Fluorescence based live cell concentration and viability for cell lines
• AO, PI, EB, DAPI, Hoechst

1. Pipette 20µL Sample	2. Insert Slide	3. Get Data

In addition to what is available with the Cellometer Auto line of cell counters, Cellometer Auto X4 has the following enhanced functionalities and capabilities:

• Brightfield (BR) and fluorescent(FL) image capabilities
• Brightfield assays for total cell count or trypan blue viability
• Fluorescence assays of DNA staining dye based cell counting for nucleated cells
• Brightfield and fluorescence combination assays for DNA staining dye or Esterase substrat based viability assays
• User switchable fluorescence optics modules for different dyes
• Software pre-installed for most common assays

Cellometer’s automatic cell counting principle is based on imaging cytometry. Cells in suspension are spread into a thin layer of liquid when loaded in a Disposable Counting Chamber. The instrument is connected to a computer via USB 2.0 connection and acquires cell images from multiple locations of the counting chamber. The software then analyzes these images and determines cell concentration, trypan viability and cell size. Results & cell images are displayed on-screen and in approximately 30 seconds.
	1. Load Counting Chamber Simply pipette 20uL into one side of a disposable counting chamber. It's nearly impossible to overload or underload, and capillary action automatically spreads your sample into a monolayer. Each slide holds two samples and feature a large, white writing surface for sample identification.
	2. Insert Silde The counting chamber is loaded into a slot on the front of the instrument. Click 'Display Image'', then 'Count' to begin counting.
	3. Get Data Less than 30 seconds later, counted images are displayed on-screen, as well as live cell count, dead cell count, concentration, and mean size. Cell size histograms and other data can be instantly displayed, and all data & images can then be saved, or exported.
Other Features
	Configuring for Different Cell Types Cellometer features more than 250 pre-programmed cell type parameters. New cell types are easily added via a New Cell Type Wizard or manual configuration. Configuring or fine tuning Cellometer for your specific assay needs is simple and straightforward, and our Support Team is always available to assist.
	Declustering Cellometer's advanced imaging can locate and accurately count cells in clumps or clusters. This feature is adjustable for your particular needs, and can also be turned off completely to count the number of clumps in a sample.
	Irregular Cell Shapes Cellometer can recognize and count irregular shaped cells by adjusting the roundness cell setting. Ideal for counting RD cells or activated T-cells.
	Nucleated Cells with Fluorescence Staining
	Total Cells and Fluorescent Cells

Cellometer Auto X4 Instrument Specifications:

Weight: 23 lbs (10kg)
Dimensions: 6"' x 8.5"' x 14'' (15cmx22cmx36cm)
Wall Adaptor: 100-240 AC, 50-60 Hz, 0.8 A
Power to instrument : 12 V DC, 2.25 A Max

Available Fluorescence Optics Modules:

Fluorescence Optics Module XB-535-401
Excitation / Emission : 470nm / 535nm

Example Fluorophores:

• Acridine Orange (+DNA)
• Calcein
• Fluorescein
• SYTO® 9
• SYTO® 13

Fluorescence Optics Module XB-595-501
Excitation / Emission : 527nm / 595nm
Example Fluorophores:

• Propidium Iodide
• Ethidium Bromide
• Rhodamine B
• SYTOX® Orange

Fluorescence Optics Module XB-450-301
Excitation / Emission : 375nm / 450nm
Example Fluorophores:

• DAPI
• Hoechst 33342 & 33258

Fluorescence Optics Module XB-695-601
Excitation / Emission: 630nm / 695nm
Example Fluorophores:

• Allophycocyanin (APC)

For customized optical modules, email support@nexcelom.com or call: 978-327-5340.