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Cellometer Vision is a line of simple to use, automated cell analyzers
with diverse functions. It combines brightfield microscopy and
multi-channel fluorescence images to generate cell count and
fluorescence data. It is most suitable for determining cell
concentration and viability in complex samples. The sophisticated data
analysis software allows detailed studies of select cell population.
Best Suited For:
- Total cell concentration for highly viable cell population
- Live cell concentration and viability using trypan blue
- Cell size measurement
- Identify and count cells based on cell size difference
- Capture and store cell images
- Total nucleated cell concentration without lysing non-nucleated cells
- Live nucleated cell concentration without lysing non-nucleated cells
- Fluorescence based live cell concentration and viability for cell lines
- Transfection efficiency by GFP
- Transfection efficiency by YFP
- Transfection efficiency by RFP
- Live cell concentration and viability using two fluorescents in one sample
- AO, PI, EB, DAPI, Hoechst
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1. Pipette 20µL Sample |
2. Insert Slide |
3. Get Data |
Cellometer Vision enables high sensitivity fluorescence detection, suitable for counting cells with reporter proteins such as GFP or RFP. Cellometer Vision is also used for measuring cells with fluorescence-conjugated antibodies.
In addition to what is available with both Cellometer Auto T4 and AutoX4, Cellometer Vision has the following enhanced functionalities and capabilities:
- Two concurrent fluorescence channels in addition to brightfield imaging
- Brightfield assays for total cell count or trypan blue viability
- Fluorescence assays of DNA staining dye based cell counting for nucleated cells
- Brightfield and fluorescence combination assays for DNA staining dye or Esterase substrat based viability assays
- Using dual fluorescent staining techniques to measure live cell concentration and viability within complex samples in a single measurement
- Detailed analysis and data presentation of fluorescence signal for each cell
- Software pre-installed for most common assays
GFP transfection |
Bright field and Fluorescence Cell Counting from the Same Sample: Cellometer Vision is capable of acquiring both bright field and fluorescent images from the same sample, making it ideal for GFP Transfection assays. By counting GFP positive cells and total number of cells, transfection rates are automatically calculated without extra data manipulation. |
Adipocytes |
Patent Pending New Method for Adipocytes Fresh, unfixed adipocyte samples can be measured for concentration and size distribution automatically. Special counting chambers are designed for the size range and cell properties of adipocytes. Fluorescence-labeled adipocytes are identified from lipid droplet background. Innovative multimode imaging techniques are used to automatically generate cell concentration and size histogram. |
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PI Viability- Cellometer Vision is configurable for rapid determination of cell concentration and viability using propidium iodide. It can also be used to determine viability using trypan blue without fluorescnece.
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Whole Blood Cells |
Minimize Sample Prep- Because generating fluorescent cell counts is now so easy, they can be more easily applied to reduce sample preparation in existing applications. Mixing whole blood sample with acridine orange and simply pipetting 20µL of sample into a disposable counting chamber is all that's needed prior to counting. White blood cells are identified and counted without lysing red blood cells.
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Easy to Use Software Cellometer Vision software is easy to use and provides just the data you need to automate common cell counting assays; Fluorescence labeled and total cell counts, cell sizes, concentration, viability percentage, as well as histograms and scatter plots can be generated with a few mouse clicks. Bright field images of counted cells as well as fluorescing cells are displayed on screen, and the software is pre-configured for hundreds of cell types, and can be customized for almost any type by the user or with Nexcelom's complimentary support. |
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Personal, Benchtop solution: With a footprint of only 6” x 8”, minimum setup or maintenance, Cellometer Vision is your personal solution for quickly and simply obtaining data. There is no need to send samples out to a core facility, or tie up a Flow Cytometer. |
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Easy to use: Utilizing advanced disposable counting chambers, only 20uL of your treated sample is required for majority of the cell types. The sample can be loaded with a standard pipette in seconds. Simply load the counting chamber into the instrument- there are no other reagents or on-board liquid handling required, eliminating the need for cleanup, maintenance or calibration. |
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Choice of imaging modes: 2 models of Cellometer Vision are available, and each is configurable for your specific application or fluorophore. The Vision Duo, features 2 imaging modes: Brightfield and your choice of one Fluorescence channel, While the Vision Trio features 3 modes: Brightfield and your choice of two Fluorescence channels. For more specific model specifications, please contact our support team. |
Cellometer® Vision's cell counting principle is based on a patent pending method combining bright field cytometry with fluorescence detection over a thin layer of cells. Image analysis software is then used to count cells, measure diameters, and quantify fluorescence values. Before now, basic cell counting with fluorescence detection required use of flow-cytometry, which typically meant advanced training, extensive sample preparation, and post-run data manipulation. This data can be generated on Cellometer Vision with just a few easy steps:
Step 1: Using a standard single channel pipette, 20µL of labeled cells in suspension are loaded into the disposable counting chamber. Capillary action spreads the cells into a thin monolayer. There is no risk of clogging, overfilling or other errors associated with on-board liquid handling systems.
Step 2: The counting chamber is inserted into the slot in the front of the instrument, and Cellometer® Vision, connected to a computer via USB 2.0 cable, acquires bright field and/or fluorescent cell images from multiple locations (Figures 1 & 2).
Step 3: Advanced image analysis software automatically analyzes the acquired images and determines cell counts, fluorescence levels and cell sizes. This data can then be viewed in a variety of outputs, such as Histograms, Scatter plots, or raw data (Figures 3, 4, 5).
Step1: Pipette 20µL |
Step 2: Insert Slide |
Step 3: Get Data |
Step 1: Using a standard single channel pipette, 20µL of labeled cells in suspension are loaded into the disposable counting chamber. Capillary action spreads the cells into a thin monolayer. There is no risk of clogging, overfilling or other errors associated with on-board liquid handling systems.
Step 2: The counting chamber is inserted into the slot in the front of the instrument, and Cellometer® Vision, connected to a computer via USB 2.0 cable, acquires bright field and/or fluorescent cell images from multiple locations (Figures 1 & 2).
Step 3: Advanced image analysis software automatically analyzes the acquired images and determines cell counts, fluorescence levels and cell sizes. This data can then be viewed in a variety of outputs, such as Histograms, Scatter plots, or raw data (Figures 3, 4, 5).
 Brightfield Image Obtained |
Figure 1: In this example, Cellometer Vision is being used to quantify the % of
cells expressing GFP reporter gene. This bright field image will be analyzed to generate
total cell count data, as well as cell size data.
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 Fluorescence Image Obtained |
Figure 2: The fluorescent image is captured next,
which will be analyzed to determine the number of cells fluorescing as well as the level of
fluorescence of each. |
 Results Displayed |
Figure 3: After image analysis, the ratio of fluorescing vs.
non-fluorescing cells is displayed. |
Quantifying GFP Transfection
Brightfield Image of HEK293 Cells
Fluorescent image of HEK293 cells.
White spots indicate GFP positive
cells.
Cellometer Vision addresses the need for simple, rapid fluorescence cell counting. By capturing both brightfield and
fluorescent images from the same cell sample, Vision can determine the total cell concentration and analyze how many
of those cells are expressing GFP. Therefore, using only 20µl of cell sample, researchers can now rapidly identify
fluorescence positive cells from a sample, analyze individual cell fluorescence intensity, calculate cell concentration,
size and determine the GFP transfection rate in minutes on the lab bench. More...
Counting WBCs in Whole Blood without Lysing
Brightfield image of whole blood.
WBC's are not visible amongst RBC's
Fluorescent image showing AO
stained WBC's. RBC's are not visible.
Vision can be used to simplify traditional methods by mixing a cell membrane permeable DNA dye (acridine orange) with
diluted whole blood sample that specifically stains white blood cells. To quantitatively analyze WBCs, we simply pipette
20µl of treated sample into a Cellometer Disposable Counting Chamber and place the chamber into the instrument.
Since the counting chamber is disposable, no washing is required between samples, there is no risk of cross contamination
of different samples, and the risk of exposure to biohazard materials is reduced. More...
Annexin-V for Detection of Apoptosis
Brightfield image of Jurkat cells.
Fluorescent image of Jurkat cells.
White spots indicate FITC labeled
annexin-V.
Cellometer Vision was developed to count total cells and identify FITC-annexin-V positive apoptotic cells based on
the microscopic and fluorescence characteristics of the cell sample. Detection typically takes less than 60 seconds
and only requires 20µl of cell sample. More...
Determination of Cell Viability with Propidium Iodide
Brightfield image of Jurkat cells.
Fluorescent image indicating PI
stained dead Jurkat cells.
Reliable and accurate viability testing can also be done using propidium iodide (PI), a fluorescent dye. Cellometer
Vision was developed to automatically count total cells and propidium iodide fluorescence labeled dead/dying cells
for fast, simple PI viability determination.
More...
Counting and Sizing Adipocytes
Brightfield Image of adipocyte sample.
Lipid droplets are indistinguishable
from intact adipocytes.
Fluorescent image showing AO
stained adipocytes. Lipid droplets
are not visible.
Using proprietary algorithms, Cellometer Vision's robust operating software accurately analyzes cell images, and
generates counting data in less than 60 seconds. Cell images and all analysis data, including cell size distribution
histograms, can be saved for documentation. Data can also be easily exported to Microsoft Excel spreadsheets for
further analysis.
More...
Counting & Calculating Viability of Hepatocytes
Brightfield image of primary human
hepotocytes. Live and dead
hepatocytes show different
morphology but are difficult to clearly
distinguish resulting in counting
variability.
After treating samples with
Fluorescence Dual Staining Solution,
AO stained live hepatocytes and PI
stained dead/dying hepatocytes are
easily distinguished in this
fluorescence image.
Nexcelom's new method incorporates staining primary hepatocytes with a ready-to-use fluorescent dual staining solution
that stains live cells with acridine orange, and dead cells with propidium iodide and then loading 20µL of labled sample
into a disposable counting chamber for analysis. Since the counting chamber is disposable, no washing is required between
samples, and the risk of cross contamination is eliminated.
More...
Determining Yeast Viability
Brightfield image of yeast cells
acquired by Cellometer Vision
Fluorescent image of oxonol
stained yeast
Use of the fluorescent dye oxonol (bis-(1,3-dibutylbarbituric acid)trimethine oxonol(DiBAC4(3)), an anionic membrane
potential dye that preferentially stains dead cells, has been reported as a reliable and accurate method to assess
yeast viability. Cellometer Vision automatically counts total and oxonol positive dead yeast cells addressing the
need for simple, rapid counting and viability testing of yeast cells. By capturing both brightfield and fluorescent
images from the same sample, the software determines total cell count and concentration and determine viability,
typically in less than 60 seconds.
More...
Cellometer Vision Instrument Specifications:
Weight: 25 lbs (11 kg)Dimensions: 6”x8.5”x14” (15cm x22cm x36cm)
Voltage: 100-240V AC 50-60 Hz
PC Computer Included
Available Fluorescence Optics Modules:
VA-535-401(Excitation / Emission: 475 nm/535 nm)
Example Fluorophores:
- Acridine Orange (+DNA)
- Alexa Fluor® 488
- Calcein
- EGFP (enhanced)
- Fluorescein (FITC)
- Green Fluorescent Protein (GFP)
- SYTO® 9
- SYTO® 13
VA-595-501
(Excitation / Emission: 525 nm/595 nm)
Example Fluorophores:
- Alexa Fluor® 546
- Alexa Fluor® 555
- Cy3®
- DsRed2 (Red Fluorescent Protein)
- Propidium Iodide
- Ethidium Bromide
- R-phycoerythrin (PE)
- Rhodamine B
- SYTOX® Orange
VA-605-501
(Excitation / Emission: 525 nm/605 nm)
Example Fluorophores:
- Alexa Fluor® 546
- Alexa Fluor® 555
- Cy3®
- DsRed2 (Red Fluorescent Protein)
- Evrogen Turbo RFP
- Propidium Iodide
- Ethidium Bromide
- R-phycoerythrin (PE)
- Red Fluorescent Protein (DsRed2)
- Rhodamine B
- SYTOX® Orange
VA-695-601
(Excitation / Emission: 630 nm/695 nm)
Example Fluorophores:
- Alexa Fluor® 647
- Allophycocyanin (APC)
- Cy5®
VA-450-301
(Excitation / Emission: 375 nm/450 nm)
Example Fluorophores:
- Alexa Fluor® 350
- BFP (Blue Fluorescent Protein)
- DAPI
- Hoechst 33342 & 33258
For customized optical modules, email support@nexcelom.com or call: 978-327-5340.
Nexcelom Bioscience LLC. | 360 Merrimack Street, Building 9 | Lawrence, MA 01843
Telephone: 978.327.5340 | Fax: 978.327.5341 | Email: info@nexcelom.com | www.nexcelom.com
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