Cellometer®: Disposable Hemacytometer

Nexcelom Bioscience's Disposable Hemacytometer is an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates.

The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.

With a pipette, 15 to 20 microlilter cell-containing sample is introduced into the counting chamber through one of the ports. Liquid spreads inside the chamber, replaces air, which escapes through the opposing port.

The cell counting chamber is placed under a microscope with a typical magnification of 100. The microscope is focused both onto the grid pattern and the cell particles. Standard protocols are followed for cell counting.

Cell counting principle using Cellometer®:

1. Count the total number of cells found in 4 large corner squares (C_total)
2. Cell concentration = Dilution x C_total / Volume

Join our free webinar. "Improving Cell Counting Data Quality & Throughput" on Friday, March 19, 2010 @ 10:00am EDT. Register now

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	Cellometer®: Disposable Hemacytometer Nexcelom Bioscience's Disposable Hemacytometer is an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates. The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed. With a pipette, 15 to 20 microlilter cell-containing sample is introduced into the counting chamber through one of the ports. Liquid spreads inside the chamber, replaces air, which escapes through the opposing port. The cell counting chamber is placed under a microscope with a typical magnification of 100. The microscope is focused both onto the grid pattern and the cell particles. Standard protocols are followed for cell counting. Cell counting principle using Cellometer®: 1. Count the total number of cells found in 4 large corner squares (C_total) 2. Cell concentration = Dilution x C_total / Volume Join our free webinar. "Improving Cell Counting Data Quality & Throughput" on Friday, March 19, 2010 @ 10:00am EDT. Register now
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