Nexcelom Bioscience

Cellometer® ViaStain™ Viability Dyes and Kits

for Cellometer Fluorescent Cell Viability Counters

Cellometer Viability Dyes

Cellometer Viability Dyes

Staining solutions for single- and dual-fluorescence concentration and / or viability determination using Cellometer cell viability counters

AO/PI Staining Solution: For accurate determination of live / dead nucleated cell concentration in heterogeneous samples using dual-fluorescence

Dual-Fluorescence Viability, using acridine orange (AO) and propidium iodide (PI), is the recommended method for accurate viability analysis of primary cells, such as PBMCs, splenocytes, and stem cells in samples containing debris and unwanted non-nucleated cell types including red blood cells.

Acridine orange (AO) and propidium iodide (PI) are nuclear staining (nucleic acid binding) dyes. AO is permeable to both live and dead cells and stains all nucleated cells to generate green fluorescence. PI enters dead cells with compromised membranes and stains all dead nucleated cells to generate red fluorescence. Cells stained with both AO and PI fluoresce red due to quenching, so all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red.

Because mature mammalian red blood cells don't contain nuclei, only live and dead mononuclear cells produce a fluorescent signal. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step.

Green fluorescent live cell image Red fluorescent dead cell image
Exclude red blood cells from count
Red blood cells seen next to the arrows in the bright field image (above left) do not appear in the fluorescent image (above right).

For AO/PI staining and viability determination, 20µl of live cell sample and 20µl of AO/PI Staining Solution are combined. 20µl of stained sample is then added to a Cellometer Counting Chamber and analyzed in <60 seconds using the Cellometer Vision or Cellometer Auto 2000 Cell Viability Counter. Each instrument automatically reports live / dead cell number, live / dead cell concentration, mean diameter, and percent viability for the sample tested.

Ordering Information

Description Instrument Compatibility Size (each) Catalog # US Price
Instruction Booklet
MSDS
Cellometer ViaStain™ AO Staining Solution
AO (acridine orange) staining solution for staining of nucleated cells.
X4, Auto 2000, K2, X2, Vision, Vision CBA 5 mL CS1-0108-5mL $100.00 PDF »
Cellometer ViaStain™ PI Staining Solution
PI (propidium iodide) staining solution for staining of dead nucleated cells.
X4, Auto 2000, K2, X1, X2, Vision, Vision CBA 5 mL CS1-0109-5mL $100.00 PDF »
Cellometer ViaStain™ AOPI Staining Solution
AO/PI (acridine orange / propidium iodide) staining solution for live/dead Mammalian nucleated cells.
Auto 2000, K2, X2, Vision, Vision CBA 5 mL CS2-0106-5mL $100.00 PDF »
25 mL CS2-0106-25mL $450.00
The ViaStain™ CFDA-AM Yeast Vitality Stain enables breweries to detect metabolically active Lager and Ale yeast at different fermentation stages. PBS included. Vision (10x), X2 200 µl CSK-0125-200µl $250.00 PDF »
Cellometer ViaStain™ Yeast Kit for Live/Dead Concentration
AO/PI Yeast Viability Kit containing dilution buffer and fluorescent dye mixture for staining of live and dead Yeast cells.
Vision (10x), X2 2 mL CSK-0102-2mL $100.00 PDF »
10 mL CSK-0102-10mL $450.00
Yeast Dilution Buffer
Yeast dilution buffer dead staining)
X4 (10x), Vision (10x), X2 500mL CS0-0110-500mL $150.00 PDF »
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