Applications

GFP Transfection:

Capable of acquiring both bright field and fluorescent images from the same sample. By counting GFP positive cells and total number of cells, transfection rate is automatically calculated without extra data manipulation.

GFP Transfection: Brightfield image
GFP Transfection: Fluorescent image
Data output showing % of cells expressing GFP

Adipocyte Count and Size Distribution

Counting unfixed adipocytes can be automated for real time results and increased reliability. Fluorescence-labeled adipocytes are identified from lipid droplet background. Patent pending multimode imaging techniques are used to automatically generate cell concentration and size histogram.

Brightfield image of Adipocytes shows little visual distinction between actual cells and lipid droplets
Flourescent image distinctly shows Adipocytes stained with Acridine Orange

Direct Count of WBC from Whole Blood

Mixing whole blood sample with acridine orange and simply pipetting 20µL of sample into a disposable counting chamber is all that's needed prior to counting. White blood cells are identified and counted without lysing red blood cells.

Brightfield image of peripheral blood sample showing RBC, WBC, and Platelets
Flourescent image showing WBC’s only

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