Nexcelom Bioscience

Cellometer Spectrum

Image Cytometry System for 20 µl Cell-Based Assays

Cellometer Spectrum Image Cytometer

Why Cellometer Spectrum?

  1. 20 µ Cell-Based Assays
  2. Unique Algorithms for Advanced Cell Analysis
  3. User-changeable Fluorescent Modules

Introduction to the Cellometer Spectrum

Whether you are looking for an affordable benchtop cytometer, or to improve your current Flow cytometry analysis, the Spectrum Image cytometry system will exceed your expectations not only in performance, data analysis and affordability, but also in its ease of use and application versatility.

All-in-One System

Basic cell counting, primary cell viability, and cell-based assays.

Dual-Fluorescence for Accurate Primary Cell Viability

No interference from red blood cells. Analyze bone marrow, peripheral blood, and cord blood without lysing.

Unique Algorithms for Advanced Cell Analysis

Determine concentration and viability of hepatocytes, adipocytes, and other sophisticated cell types.

Fast Results

Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

Simple 20 µl Cell-Based Assays

GFP | Apoptosis | Cell Cycle | Cell Mediated Cytotoxicity | Cell Counting & Viability

  • Accurate Cell Counting
  • Versatile Analysis and Applications
  • Pre-qualified Reagents
  • Pre-defined Instrument Settings
  • Assay-Specific data templated
  • Export to FCS Express* (Flow like data analysis)
Simple Cell-Based Assays

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Cell Types for Many Research Areas

Cellometers are optimized for primary cell analysis

Stem Cells
Neural Cells
Dendritic Cells

Epithelial Cells

  • Clinical Immunology: PBMCs
  • Diabetes / Obesity: Adipocytes
  • Immunotherapy: Leukocytes
  • Microbiology: Yeast (Spectrum 10x)
  • Oncology: Cell Lines
  • Regenerative Medicine: Stem Cells
  • Toxicology: Hepatocytes
  • Transplantation: Nucleated Cells
  • Vaccine Development: Splenocytes

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Accurate Cell Counting

Bright Field

Bright field cell image

Stained AO+ Cells

Stained AO + Cells

Counted AO+ Cells

Counted AO + Cells
Cellometer Spectrum Dynamic Range
  • Automatic identification of Acridine Orange (AO) stained cells (shown above)
  • Image and count up to 1.51x107 cells/mL
  • Image acquisition and analysis: 30 seconds/sample for a 3-channel assay
  • Versatile platform - use for routine cell counting and complex cell-based assays

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Export to FCS Express* for Flow-Like Data

Counted PI+ Cells

Counted PI + Cells

Contour Population Plot

Contour population plot
  • Export image data into flow cytometry software: FCS express
  • Simple work flow: No fluid- stream, No PMT voltages, No forward/side scatter
  • Easily perform data analysis using pre-designed templates
  • Quickly plot cell population data as a: histogram, scatter plot, dot plot or contour plot
PI Cell cycle data

* FCS Express Flow Cytometry software is a product of De Novo Software and is included with the Cellometer Spectrum

Cell Population % of Gated Cells Concentration
(10^6 cells/mL)
Total 100 9.2
Sub G1 4.7 0.1
G0/G1 58.0 5.8
S 13.4 1.3
G2/M 23.9 1.9

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User-Changeable Fluorescence Optics Modules*

Cellometer assays use specific optic modules for maximum performance and discrimination between fluorescence channels. Standard modules are listed in the table below. Custom fluorescence optics modules are also available.

Optics Module Fluorophores Nucleic Acid Stains
Ex: 375 nm
Em: 450 nm
AlexaFluor® 350
Hoechst 33342
Hoechst 33258
Ex: 475 nm
Em: 535 nm
AlexaFluor® 488
AO (acridine orange)
Ex: 525 nm
Em: 595 nm
546 AlexaFluor® 555, Cy3®
PE (R-phycoerythrin)
Rhodamine B
PI (propidium iodide)
EB (ethidium bromide)
SYTOX® Orange
Ex: 540 nm
Em: 660 nm
Nile Red
PI (propidium iodide)
EB (ethidium bromide)
Ex: 630 nm
Em: 695 nm
AlexaFluor® 647, Cy5®
APC (allophycocyanin)

*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Nexcelom technical support regarding compatibility of other reagents.
Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.

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