Nexcelom Bioscience

978-327-5340

Proliferation/Tracer Reagents View all Nexcelom reagents →

Instrument Compatibility for Proliferation/Tracer Reagents

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X1
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K2
Vision CBA
Celigo
ViaStain™ Calcein AM X X   X X X X
ViaStain™ CFSE             X
ViaStain™ Tracer Blue             X
ViaStain™ CMFDA             X

ViaStain™ Calcein AM

Nexcelom ViaStain Calcein-AM

Calcein AM (Calcein acetoxymethyl ester) is a cell permeable, non-fluorescent compound. Upon crossing the cell membrane, calcein-AM is rapidly hydrolyzed by cellular esterases inside live cells. The hydrolysis cleaves the AM group, converting the non-fluorescent calcein AM to a strongly green fluorescing calcein. Because the created calcein is more hydrophilic it is trapped inside the cells with intact membranes. Cells that do not possess active cytoplasmic esterases are unable to convert calcein AM to calcein, and therefore do not fluoresce green. This allows for a quick and easy detection of metabolically-active cells in a sample.

Since calcein-AM does not require DNA binding, it stains all metabolically-active cells and can be used to measure metabolic activity in non-nucleated cells, such as platelets. Because calcein AM is photostable, has low cytotoxicity and does not affect cellular functions, it is a popular stain for the examination of cell vitality and metabolic activity.

ViaStain™ Calcein AM

Description Catalog number Unit size Price (USD) Manual MSDS

Cell permeable viability dye. In live cells, non-fluorescent Calcein AM is converted by cellular esterases into green fluorescing cells.

CS1-0119 200 uL $250.00 -- -- Buy Online

ViaStain™ CFSE

Nexcelom ViaStain™ CFSE is a cell permeable, non-fluorescent compound. Upon crossing the cell membrane, CFSE is rapidly hydrolyzed by cellular esterases inside live cells converting it from a non-fluorescent to a strongly green fluorescing dye. CFSE then covalently binds to intracellular proteins, making it a good candidate for multi-day cell labeling. Upon cell division, approximately half of the fluorescent label is passed onto the daughter cell. After each successive cell division the fluorescent intensity of the dye is reduced by half and the staining appears proportionately dimmer. The dye becomes difficult to detect after approximately 3-5 generations.

ViaStain™ CFSE

Description Catalog number Unit size Price (USD) Manual MSDS

Green cell proliferation dye. Upon cell division in labeled cells, each daughter cell receives half the dye that is present in the parent cell.

CS1-P0002-1 -- $120.00 -- -- Buy Online

ViaStain™ Tracer Blue

The Tracer Blue is designed to uniformly label live cells in the blue fluorescence channel. The non-fluorescing blue dye crosses intact cell membranes, and is then converted from non-fluorescent to a strongly fluorescing blue dye. The more hydrophilic fluorescent molecules are trapped inside the cells with intact membranes making the dye ideal for multi-day cell tracking.

ViaStain™ Tracer Blue

Description Catalog number Unit size Price (USD) Manual MSDS

Tracer Blue dye is a UV wavelength cell tracer dye that is retained inside live cells and is used for cell labeling.

CS1-P0003-1 -- $195.00 -- -- Buy Online

ViaStain™ CMFDA

ViaStain™ CMFDA (5-Chloromethylfluorescein diacetate) is chemically same molecule as CellTrackerâ„¢ Green CMFDA (trademark of Invitrogen). CMFDA is a cell permeable, non-fluorescent compound. Upon crossing the cell membrane, it is rapidly hydrolyzed by cellular esterases inside live cells converting it from a non-fluorescent to a strongly green fluorescing dye. Upon hydrolysis the more hydrophilic CMFDA is trapped inside the cell until cell division. Upon cell division, approximately half of the fluorescent label is passed onto the daughter cell. After each successive cell division the fluorescent intensity of the dye is reduced by half and the staining appears proportionately dimmer. The dye becomes difficult to detect after approximately 3-5 generations.

ViaStain™ CMFDA

Description Catalog number Unit size Price (USD) Manual MSDS

CMFDA is a green cell tracer dye that is retained inside live cells and is used for cell labeling.

CS1-P0001-1 -- $195.00 -- -- Buy Online
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