Application Notes

• New: Development of a Novel Method to Assess Primary Hepatocyte Concentration and Viability PDF
• New: Yeast Concentration and Viability using Image-Based Fluorescence Analysis PDF
• Automated Methods for Counting and Analyzing Stem Cell Samples PDF
• Quantitative Measurement of GFP Transfection Rates in 60 Seconds - Cellometer Vision PDF
• Direct Count of White Blood Cells from Peripheral Blood Sample
  without Lysing Red Blood Cells - Cellometer Vision PDF
• Rapid Detection of Apoptosis in Jurkat Cells with FITC Conjugated Annexin-V - Cellometer Vision PDF
• Simple, Fast Determination of Viability by Staining Cells with Propidium Iodide - Cellometer Vision PDF
• Automated Counting and Sizing Freshly Isolated Adipocytes with
  Minimal Sample Preparation - Cellometer Vision PDF
• PBMC Counting by Cellometer Auto T4 PDF
• Insect Cell Counting and Size Analysis Using Cellometer Auto T4 PDF
• Counting Cells for Immunology Using Cellometer Auto T4 Cell Counter PDF
• Cellometer Auto T4 Cell Counting Consistency PDF
• Cellometer Tips for Counting Clumpy Cells PDF
• Counting Yeast, Human Platelet, and Algae Using Cellometer Auto M10 PDF.
• Cellattice: Monitoring Primary Neuron Growth and Development PDF

Publications and Posters

• Cytometry Part A, April 2011
  Cellometer Vision as an alternative to flow cytometry for cell cycle analysis, mitochondrial potential, and immunophenotyping
  
  Leo L. Chan, Xuemei Zhong, Jean Qiu, Peter Y. Li, Bo Lin
  
  Cell phenotyping and cell cycle analysis are two commonly used assays in both clinical diagnosis and biomedical research. Cell phenotyping by identifying different biomarkers is essential for the diagnosis of hematologic malignancy, sub-classifying diseases, monitoring response to treatment, predicting prognosis, detecting rare cell populations and residual malignant cells. Read More


• Journal of Pharmacological and Toxicological Methods, November 2010
  Rapid detection of ABC transporter interaction: Potential utility in pharmacology.
  
  Robey RW, Lin B, Qiu J, Chan LL, Bates SE
  
  We have developed a method to characterize the function and inhibition of ABC transporters using an automated cell counter with fluorescence detection capability. The assay was performed using stably-transfected HEK293 cells expressing P-gp, MRP1, or ABCG2 and examining transport of fluorescent substrates in the presence or absence of known inhibitors and compared to results obtained with a flow cytometer.


• Journal of Industrial Microbiology & Biotechnology, October 2010
  Direct concentration and viability measurement of yeast in corn mash using a novel imaging cytometry method.
  
  Chan LL, Lyettefi EJ, Pirani A, Smith T, Qiu J, Lin B.
  
  Worldwide awareness of fossil-fuel depletion and global warming has been increasing over the last 30 years. Numerous countries, including the USA and Brazil, have introduced large-scale industrial fermentation facilities for bioethanol, biobutanol, or biodiesel production. Most of these biofuel facilities perform fermentation using standard baker's yeasts that ferment sugar present in corn mash, sugar cane, or other glucose media. In research and development in the biofuel industry, selection of yeast strains (for higher ethanol tolerance) and fermentation conditions (yeast concentration, temperature, pH, nutrients, etc.) can be studied to optimize fermentation performance. Yeast viability measurement is needed to identify higher ethanol-tolerant yeast strains, which may prolong the fermentation cycle and increase biofuel output. In addition, yeast concentration may be optimized to improve fermentation performance. Therefore, it is important to develop a simple method for concentration and viability measurement of fermenting yeast. In this work, we demonstrate an imaging cytometry method for concentration and viability measurements of yeast in corn mash directly from operating fermenters. It employs an [Cellometer] automated cell counter, a dilution buffer, and staining solution from Nexcelom Bioscience to perform enumeration. The proposed method enables specific fluorescence detection of viable and nonviable yeasts, which can generate precise results for concentration and viability of yeast in corn mash. This method can provide an essential tool for research and development in the biofuel industry and may be incorporated into manufacturing to monitor yeast concentration and viability efficiently during the fermentation process.Read more...
  
  
• New Poster:
  A Rapid Alternative Method for Cell Cycle Analysis Using Cellometer Vision PDF
• New Poster:
  A Novel Imaging Cytometry Method for Immunophenotyping PDF
• New Poster:
  Apoptosis Analysis of Jurkat Cells using the Cellometer® Vision PDF
• New Poster:
  Concentration and Viability Measurement of Yeast in Corn Mash using the Cellometer® Vision PDF
• Poster: Concentration and Viability Measurement of PBMC using the Cellometer® Vision PDF
• Poster: Obtaining Consistent and Accurate Cell Counting Results with Cellometer Automatic Cell Counters PDF

Product Literature

• Cellometer® Auto T4 Product Flyer PDF
• Cellometer® Auto X4 for Yeast Product Flyer PDF
• Cellometer® Vision Product Flyer PDF
• Cellometer® Vision CBA Product Flyer PDF
• Cellometer® Auto Spec Sheet PDF
• Cellometer® Auto X4 Spec Sheet PDF
• Cellometer® Auto 2000 Spec Sheet PDF
• Cellometer® Vision CBA Spec Sheet PDF
• Running Cellometer on an Intel Mac PDF
• Cellometer® Vision Product Literature PDF
• Cellometer Auto T4 Cell Counting Comparison to Hemacytometer PDF
• Cellometer® Auto T4 Product Literature PDF
• Cellometer® Auto T4 Technical Information PDF
• Cellometer AutoM10 Product Literature PDF
• Disposable Hemacytometer Product Literature PDF
• Disposable Hemacytometer Technical Data PDF