Cellometer in The News: Recent Publications Referencing Cellometer Automated Cell Counters

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Below are the most recent publications referencing Cellometer, if you would like to see your research listed here, just let us know...events@nexcelom.com

2010

	Cancer Genetics and Cytogenetics, Jan 2010 UOK 262 cell line, fumarate hydratase deficient (FH-/FH-) hereditary leiomyomatosis renal cell carcinoma: in vitro and in vivo model of an aberrant energy metabolic pathway in human cancer Youfeng Yang, Vladimir A. Valera, Hesed M. Padilla-Nash, Carole Sourbier, Cathy D. Vocke, Manish A. Vira, Mones S. Abu-Asab, Gennady Bratslavsky, Maria Tsokos, Maria J. Merino, Peter A. Pinto, Ramaprasad Srinivasan, Thomas Ried, Len Neckers, W. Marston Linehan ...Cells in particular wells were counted in triplicate at 24-hour intervals using a Cellometer auto T4 image-based cell counter (Nexcelom Bioscience, ...

	Chemical Research in Toxicology, Jan 2010 DNA Interstrand Cross-Linking Activity of (1-Chloroethenyl)oxirane, a Metabolite of β-chloroprene Brian A. Wadugu, Christopher Ng, Bethany L. Bartley, Rebecca J. Rowe and Julie T. Millard ...dyed samples were monitored for viable cells with an automated cell counter (Cellometer Auto T4, Nexcelom Bioscience), and the viable fraction was plotted versus concentration...

	Stem Cells and Development, Jan 2010 Cell Growth Characteristics, Differentiation Frequency, and Immunophenotype of Adult Ear Mesenchymal Stem Cells Jaroslaw Staszkiewicz, Trivia P. Frazier, Brian G. Rowan, Bruce A. Bunnell, Ernest S. Chiu, Jeffrey M. Gimble, Barbara Gawronska-Kozak ...exclusion and Cellometer Auto T4 system (Nexcelom Bioscience LLC, Lawrence, MA) were used to determine the vitality, size and the total number of nucleated ...

	ASSAY and Drug Development Technologies, Jan 2010 The Pyruvic Acid Analog 3-Bromopyruvate Interferes With the Tetrazolium Reagent MTS in the Evaluation of Cytotoxicity Shanmugasundaram Ganapathy-Kanniappan, Jean-Francois H. Geschwind, Rani Kunjithapatham, Manon Buijs, Labiq H Syed, Pramod P. Rao, Shinichi Ota, Mustafa Vali ...Cell counting was performed either manually or by using Nexcelom counter as described in Materials and Methods. View larger version (30K). Fig. 2. ...

	Clinical Cancer Research, Feb 2010 Monitoring Drug-Induced γH2AX as a Pharmacodynamic Biomarker in Individual Circulating Tumor Cells Lihua H. Wang,Thomas D. Pfister, Ralph E. Parchment, Shivaani Kummar,Larry Rubinstein, Yvonne A. Evrard, Martin E. Gutierrez, Anthony J. Murgo, Joseph E. Tomaszewski, James H. Doroshow, Robert J. Kinders ... Type Culture Collection) and gentamicin sulfate (Biowhittaker). Cells were counted using the Cellometer Automatic Cell Counter (Nexcelom Bioscience). ...
2009

	GENERAL ENDOCRINOLOGY: Endocrinology, Jan 2009 Identification and Characterization of Nesfatin-1 Immunoreactivity in Endocrine Cell Types of the Rat Gastric Oxyntic Mucosa Andreas Stengel, Miriam Goebel, Iskandar Yakubov, Lixin Wang, Derrick Witcher, Tamer Coskun, Yvette Taché, George Sachs, et al ...mucosal small endocrine cells were isolated as described above. Cells were counted (Cellometer Disposable Cell Counting Chamber; Nexcelom Bioscience Lawrence, MA) and 400,000 cells incubated in lysis buffer 0.15 m NaCl, 1% Triton X-100, 4 mm EGTA in 15 mm Tris...

	EXPERIMENTAL THERAPEUTICS, MOLECULAR TARGETS, AND CHEMICAL BIOLOGY: Cancer Research, Jan 2009 Targeting Ornithine Decarboxylase Impairs Development of MYCN-Amplified Neuroblastoma Robert J. Rounbehler, Weimin Li, Mark A. Hall, Chunying Yang, Mohammad Fallahi, and John L. Cleveland ...of DFMO-treated cells were individually collected and counted. Cells were counted using a Cellometer Auto T4 cell counter (Nexcelom Bioscience). Mice and tumor analyses. Mice expressing the human MYCN gene under the control of the rat tyrosine hydroxylase...

	HEAD AND NECK CANCER: Journal of Clinical Oncology, March 2009 Intratumoral Epidermal Growth Factor Receptor Antisense DNA Therapy in Head and Neck Cancer: First Human Application and Potential Antitumor Mechanisms Stephen Y. Lai, Priya Koppikar, Sufi M. Thomas, Erin E. Childs, Ann Marie Egloff, Raja R. Seethala, Barton F. Branstetter, William E. Gooding, et al ... After 24 hours, viable cells were counted on the Nexcelom automated cell counter (Nexcelom Bioscience, Lawrence, MA). The cell proliferation experiments were independently performed four times...

	CELLULAR IMMUNOLOGY AND IMMUNE REGULATION:Journal of Immunology, March 2009 Cell-Autonomous Role for NF-B in Immature Bone Marrow B Cells Estefania Claudio, Sun Saret, Hongshan Wang, and Ulrich Siebenlist ...cells were counted with a Cellometer Vision Trio cell counter and the data were analyzed with Cellometer vision 4 software from Nexcelom Bioscience. Real-time PCR Total RNA was extracted from purified immature B cells and DNase-treated...

	INFLAMMATION: Journal of Immunology, May 2009 Neisseria gonorrhoeae Activates the Proteinase Cathepsin B to Mediate the Signaling Activities of the NLRP3 and ASC-Containing Inflammasome Joseph A. Duncan, Xi Gao, Max Tze-Han Huang, Brian P. O'Connor, Christopher E. Thomas, Stephen B. Willingham, Daniel T. Bergstralh, Gary A. Jarvis, et al ...sample) and counted using the automated Cellometer Auto T4 (Nexcelom Bioscience) according to the manufacturers protocols. Cell...blue and the percentage of viable cells was counted using the Nexcelom Cellometer Auto T4. B, Culture supernatants from infected cells...

	MECHANISMS OF SIGNAL TRANSDUCTION: Journal of Biological Chemistry, May 2009 Genomic Responses from the Estrogen-responsive Element-dependent Signaling Pathway Mediated by Estrogen Receptor Are Required to Elicit Cellular Alterations Stephanie L. Nott, Yanfang Huang, Xiaodong Li, Brian R. Fluharty, Xing Qiu, Wade V. Welshons, ShuYuan Yeh, and Mesut Muyan ...Cells were collected and counted using a hemocytometer (Hausser Scientific, Horsham, PA) and/or an automated cell counter (Nexcelom Biosciences, Lawrence, MA) or subjected to MTT assay as described previously (12). For the effects of ERs on U-2OS cell proliferation...

	CELLULAR IMMUNOLOGY AND IMMUNE REGULATION: Journal of Immunology, May 2009 Chronic Lymphocytic Leukemia and B and T Cells Differ in Their Response to Cyclic Nucleotide Phosphodiesterase Inhibitors John A. Meyers, Derrick W. Su, and Adam Lerner ...Results were normalized to exact cell concentration as determined by machine count (Nexcelom Bioscience). Because of a high degree of variability between donors, data from drug-treated samples were expressed relative...

	INFLAMMATION, CYTOKINES, NEUROIMMUNE INTERACTIONS: American Journal of Physiology, June 2009 High glucose concentration in cell culture medium does not acutely affect human mesenchymal stem cell growth factor production or proliferation Brent R. Weil, Aaron M. Abarbanell, Jeremy L. Herrmann, Yue Wang, and Daniel R. Meldrum ...Viable cells, as determined by the ability to exclude trypan blue, were then counted via the aid of an automated cell counter (Nexcelom Bioscience, Lawrence, MA). Proliferation. Proliferation was assayed with the use of the 5-Bromo-2-deoxy-uridine Labeling...

	ATHEROSCLEROSIS/LIPOPROTEINS: Atherosclerosis, Thrombosis and Vascular Biology, Jul 2009 Thiol Oxidative Stress Induced by Metabolic Disorders Amplifies Macrophage Chemotactic Responses and Accelerates Atherogenesis and Kidney Injury in LDL Receptor-Deficient Mice Mu Qiao, Qingwei Zhao, Chi Fung Lee, Lisa R. Tannock, Eric J. Smart, Richard G. LeBaron, Clyde F. Phelix, Yolanda Rangel, and Reto Asmis et al ...The plugs were removed at the time of sacrifice, dissolved, and cells were counted in an automated video-based hemocytometer (Nexcelom). Cell staining with antibodies directed against macrosialin/CD68 confirmed that 93% of the cells recruited...

	Applied and Environmental Mircobiology, July 2009 Quantitative PCR for genetic markers of human fecal pollution Orin C. Shanks, Catherine A. Kelty, Mano Sivaganesan, Manju Varma, and Richard A. Haugland ...washed suspensions were determined by 145 bright field microscopy at 40x magnification in disposable hemocytometer chambers146 (Nexcelom Bioscience, #CP2-002, Lawrence, MA). DNA was isolated from the cell147 suspensions using a bead beating extraction approach...

	The Journal of Cell Biology, Aug 2009 The Dictyostelium type V myosin MyoJ is responsible for the cortical association and motility of contractile vacuole membranes Goeh Jung, Margaret A. Titus, and John A. Hammer, III ...dominant-negative, GFP-GTD construct, cells were incubated as above for 0 and 3 h in water and counted in triplicate using an Auto T4 cellometer (Nexcelcom Bioscience). Cells were also stained with Trypan blue to identify dead cells that were still intact. Comparison...

	Journal of Neurosurgery: Pediatrics, Dec 2009 Longitudinal assessment of regional directed delivery in a rodent malignant glioma model Akihide Kondo, Stewart Goldman, Rishi R. Lulla, Barbara Mania-Farnell, Elio F. Vanin, Simone T. Sredni, Veena Rajaram, Marcelo B. Soares, Tadanori Tomita ...The cell suspension was counted with an auto cell counter (Cellometer Auto T4, Nexcelom Bioscience LLC) and centrifuged at 1000 × G for 5 minutes. ...

	Experimental Oncology, Dec 2009 THE EFFECT OF RACEMIC GOSSYPOL AND AT-101 ON ANGIOGENIC PROFILE OF OVCAR-3 CELLS: A PRELIMINARY MOLECULAR FRAMEWORK FOR GOSSYPOL ENANTIOMERS U. Varol, B. Karaca1, D. Tunali, M. Degirmenci, Y. Cirak, D.U. Purcu, S. Uzunoglu, C. Sezgin, B. Karabulut, U. A Sanli, R. Uslu ...After verifying cell viability using trypan blue dye exclusion test by Cellometer automatic cell counter (Nexcelom Inc.,USA.), cells were seeded at approximately...
2008

	MEETING-ABSTRACT: Investigative Ophthalmology & Visual Science, April 2008 Heat Shock Mitigates Sodium Iodate-Mediated RPE Cell Death S. J. Upadhyay, J. A. Hossain, C. Leeuwenburgh, and S. Kaushal ...to cells at various concentrations. At various times afterwards, cells were harvested and viability was determined using a cellometer after trypan blue staining. An experimentally convenient dose (10 mM) was established and used in all subsequent studies...

	MEDICAL SCIENCES: Proceedings of the National Academy of Sciences, April 2008 Bromodomain 4 activation predicts breast cancer survival Nigel P. S. Crawford, Jude Alsarraj, Luanne Lukes, Renard C. Walker, Jennifer S. Officewala, Howard H. Yang, Maxwell P. Lee, Keiko Ozato, et al ...the indicated periods of time. After incubation, the cells were harvested, and the number of cells was determined by using a cellometer. Spontaneous Metastasis Assays. Transfected cells proven to be stably expressing Brd4 were s.c. implanted into virgin...

	Journal of Hypertension, May 2008 Inhibition of cardiovascular cell proliferation by angiotensin receptor blockers: are all molecules the same? Benson, Stephen C; Iguchi, Rumiko; Ho, Christopher I; Yamamoto, Koichi; Kurtz, Theodore W ...We changed the media containing the drugs every 2 - 3 days and then measured proliferation after 7 days by counting the cells with a Nexcelom Auto T4 ...

	METHODS: Applied and Environmental Mircobiology, Aug 2008 Rapid Ultrafiltration Concentration and Biosensor Detection of Enterococci from Large Volumes of Florida Recreational Water Stephaney D. Leskinen and Daniel V. Lim ...bicarbonate (CBC) buffer (10.59 g Na2CO3 and 8.40 g NaHCO3 per liter [pH 9.3]). Direct counts were performed using a Cellometer (Nexcelom Bioscience, Lawrence, MA), and the concentration of each cell suspension was adjusted to 1.0 109 cells/ml. The suspensions...

	IMMUNOBIOLOGY: Blood, Oct 2008 Exogenous insulin-like growth factor 1 enhances thymopoiesis predominantly through thymic epithelial cell expansion Yu-Waye Chu, Sabrina Schmitz, Baishakhi Choudhury, William Telford, Veena Kapoor, Susan Garfield, David Howe, and Ronald E. Gress ...after 2 rounds of ACK lysis. Cells were then counted with a hemocytometer or using a Cellometer Auto T4 automated cell counter (Nexcelom Bioscience, Lawrence, MA). For flow cytometry, cells were incubated with 2.4G2 blocking agent followed by directly conjugated...

	MECHANISMS OF SIGNAL TRANSDUCTION: Journal of Biological Chemistry, Nov 2008 Effects of the JAK2 Inhibitor, AZ960, on Pim/BAD/BCL-xL Survival Signaling in the Human JAK2 V617F Cell Line SET-2 Joseph M. Gozgit, Geraldine Bebernitz, Pankaj Patil, Minwei Ye, Julie Parmentier, Jiaquan Wu, Nancy Su, Tao Wang, Stephanos Ioannidis, et al ...described above. Twenty-four hours after transfection cell number and viability were determined using the Cellometer Auto T4 (Nexcelom Biosciences, Lawrence, MA), cells were plated at 20,000 cells/well in 96-well plates, and incubated for...

2007

	RESEARCH ARTICLES: Development, Mar 2007 Sperm binding to the zona pellucida is not sufficient to induce acrosome exocytosis Boris Baibakov, Lyn Gauthier, Prue Talbot, Tracy L. Rankin, and Jurrien Dean ... After washing the column with M16 media (2 ml), sperm were collected in a second fraction (4 ml) and counted on a cellometer (Nexcelom Bioscience, Lawrence, MA). The sperm penetration assay chamber...

	TRICHOMONAS: Sexually Trasmitted Infections, April 2007 Comparison of a TaqMan-based real-time polymerase chain reaction with conventional tests for the detection of Trichomonas vaginalis A Pillay, F Radebe, G Fehler, Y Htun, and R C Ballard ...strains were grown anaerobically in 12 ml of Diamonds medium at 35C for 48 h. Trichomonads were counted using a Cellometer Nexcelom Bioscience, Lawrence, Massachusetts, USA, and genomic DNA was extracted...

	METHODS: Applied and Environmental Mircobiology, Oct 2007 Development of Quantitative Real-Time PCR Assays for Detection and Quantification of Surrogate Biological Warfare Agents in Building Debris and Leachate Pascal E. Saikaly, Morton A. Barlaz, and Francis L. de los Reyes, III ...min. The resulting pellets were resuspended in sterile water, and total bacterial counts were determined using a Cellometer (Nexcelom Bioscience, Lawrence, MA) and phase-contrast microscope at a magnification of 40. In addition, four replicate B. atrophaeus...

	RESEARCH ARTICLES: Physiological Genomics, Oct 2007 Identification of genomic targets downstream of p38 mitogen-activated protein kinase pathway mediating tumor necrosis factor-α signaling Cindy Zer, George Sachs, and Jai Moo Shin ...The cells from each plate were isolated and counted with a Cellometer (Nexcelcom Bioscience). The concentration of Cinc2a and Cinc2b...ml of DMEM with 1 FCS, and cell number was counted with a Cellometer. PMN chemotaxis. The supernatants of TNF-a- or vehicle-treated...

	MOLECULAR BASIS OF CELL AND DEVELOPMENTAL BIOLOGY: Journal of Biological Chemistry, Oct 2007 Identification and Biochemical Characterization of Unique Secretory Nucleases of the Human Enteric Pathogen, Entamoeba histolytica Glen C. McGugan, Jr., Manju B. Joshi, and Dennis M. Dwyer ...cultures were taken at regular intervals, diluted appropriately, and counted using disposable cell counting chambers (Cellometer, Nexcelom Bioscience, Lawrence, MA). Temporal Release Assays-Temporal release assays were used to determine whether transfected trophozoites...

	POSTER SESSION: Blood (ASH Annual Meeting Abstracts), Nov 2007 Iron Chelation in Mantle Cell Lymphoma Heather M. Gilbert, Josef T. Prchal, and Miles C. Deneris ...PIH), and Salicylaldehyde Isocotinoyl Hydrazone (SIH). We then measured and compared cell cycle proliferation (using the Cellometer Auto T4, an instrument that measures cell count, cell viability, and cell size) and the rate of apoptosis (via propidium iodide...