Cell-Based Fluorescent Assays in Micro-Well Plates

Fluorescent Assays
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Assay Protocols & Demos

Cell Cycle

Image and distinguish cell cycle phases using Propidium Iodide (PI), BrdU/DAPI and EdU/DAPI.

Viability/Cell Health

Quick and easy plate-based viability methods: calcein AM/PI/Hoechst, Hoechst/PI and AO/PI.

Internalization & Phagocytosis

Detect and measure receptor or antibody internalization and phagocytosis via fluorescent detection.

Co-Culture

Image and perform population analysis for GFP/RFP or other multi-color assays.

Surface Proteins & Antibodies

Detect and enumerate fluorescently-labeled cell surface antibodies or proteins.

Transfection/Transduction

Quantify transfection and transduction efficiencies by direct cell counting.

Cell Secretion

Quantitative fluorescent analysis of protein secretion from individual cells.

Apoptosis

Perform kinetic and endpoint apoptosis experiments using caspase 3/7 and annexin V.

Wound Healing

Measure migration of fluorescently-labeled cells over time in a wound healing migration assay.