Advanced Yeast Analyses
Measuring the count, concentration, viability and vitality of yeast are the most common measurements performed. By using fluorescence based detection methods the Cellometer Vision 10X instrument can also measure the glycogen content, neutral lipid content, trehalose content and GFP transfection efficiency of cultured yeast samples.
Advanced Fluorescent Assays Measuring Yeast Physiologic Parameters
Yeast Vitality Analysis Using CFDA-AM & Propidium Iodide
Yeast Glycogen Content Using Acriflavine Fluorescent Staining
Yeast glycogen is a macromolecule that provides the energy and carbohydrates required for yeast sterols and lipid synthesis, which ensures yeast metabolism during fermentation. Glycogen content can be measured by staining with acriflavine (using FOM VB-535-402) and analyze fluorescence intensity of individual cells in a fluorescent histogram. Glycogen has been shown to correlate to vitality and is an essential physiological parameter to be monitored.
Yeast Neutral Lipid Content Using Nile Red Fluorescent Staining
Neutral lipids are energy rich molecules that are stored in yeasts, which cannot be metabolized. However, it can protect yeast cells from toxic substances like ethanol, which can prolong survival capacity. In order to measure neutral lipids, the yeasts can be stained with Nile Red (using FOM VB-595-502), and analyze fluorescence intensity of individual cells in a fluorescent histogram. The fluorescent linear gating was set to measure the population percentage of high Nile Red fluorescence.
Yeast Trehalose Content Using Concanavalin A-FITC Fluorescent Staining
Trehalose is a sugar disaccharide that supplies energy during cell cycle, or cell proliferation. Similar to neutral lipids, trehalose can protect yeast cells against stress, high ethanol content, heat, dehydration, oxidation, pH change. In order to measure trehalose content, the yeast cells are stained with concanavalin A-FITC (using FOM VB-535-402), and analyze fluorescence intensity of individual cells in a fluorescent histogram. Trehalose are also known to increase survival capacity during fermentation. The fluorescent linear gating was set to measure the population percentage of high FITC fluorescence.
Yeast Green Fluorescent Protein (GFP) Transfection
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Cellometer References for Yeast Analysis
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