Compared to 2D monolayers, 3D cell structure environments more closely resemble in vivo characteristics in terms of gene expression, responses to treatment, and other characteristics. From producing uniform spheroids to gathering accurate multi-dimensional data, the Perkin Elmer hosted webinar series, “Towards Physiological Relevance: High-Content Analysis with 3D Organoids and Spheroids” promises to address several complex challenges associated with intricate 3D microenvironments and cell model systems.
Developing a 3D Cell Model for High-Content Analysis
Thursday, September 2, 2021
Developing a 3D Cell Model for High-Content Analysis is presented by cellular imaging and analysis experts Dr. Joe Trask of PerkinElmer and Dr. Antti Hassinen from the Institute for Molecular Medicine Finland. The introductory discussion includes background information as well as the preliminary rationale for interrogating 3D cell model systems in the research and development process. The second demonstration shows how 3D image analysis of cells and organoids gathers multiparametric information to further characterize patient sample readouts for superior personalized medicine as well as drug sensitivity and resistance testing.
High-Throughput, High-Content Screening with 3D Cell Models
Thursday, September 9, 2021
High-Throughput, High-Content Screening with 3D Cell Models is put forth by professors Dr. Ben Hopkins of the Mount Sinai School of Medicine in New York and Dr. Dan LaBarbera from the University of Colorado Anschutz Medical Campus. Discussions relate functionally tests of patient-derived models to genetic events that give rise to and sustain tumors. Additional thoughts revolve around understanding mesenchymal cell plasticity to determine if select precision oncology workflows can be used to guide future clinical decision-making.
Working with Complex 3D Cell Models
Thursday, September 16, 2021
Working with Complex 3D Cell Models is exhibited by tissue engineering specialist Dr. Marianne Kanellias of Emulate in Boston and laboratory head Dr. Jan Bruder of the Max Planck Institute for Molecular Biomedicine in Münster, Germany. This webinar emphasizes the complexities of 3D human organ-like cell culture systems and how organoids can potentially generate more relevant models of human physiology and disease. This talk also discusses the feasibility of quantitatively assessing cell-type-specific toxicity of in vitro human organoids at the single-cell level in a scalable framework compatible with high-throughput analysis approaches.
Deriving Insights from 3D Cell Model Systems
Thursday, September 23, 2021
Deriving Insights from 3D Cell Model Systems is performed by associate professor Dr. Eric Darling from Brown University in Providence, RI, and high-content imaging and screening expert Dr. James Hutt of PerkinElmer, UK. The first talk will describe a versatile approach to using fluorescence-based imaging to measure the microscale mechanical cellular forces during tissue formation. The final discussion explores how to gain insightful discoveries using 3D volumetric analysis to better understand 3D cell models.
Each presentation offers a dialogue with subject matter experts for gaining insights into working directly with complex 3D structures. Learn more by reading the individual speaker biographies and full webinar abstracts on the registration page.