celigo-3D-assay-validationU87MG cells were used to create tumorspheres in 384-well plates that were subsequently analyzed by imaging. The data illustrate that reproducible 3D spheroids can be formed in 384-well plates. Fluorescent viability studies were carried out with the imager using pixel intensity analysis. Moreover, the assay was validated for drug screen using various drug compounds that have shown anti-proliferative effects. Together, these data demonstrate that the tumorsphere formation assay can be developed, validated and used for high-throughput anti-cancer compound screening in 384-well, U-bottom low-attachment plates using the Celigo Imaging Cytometer.

3D tumor spheroid
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