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Cellometer Auto T4 Bright Field Cell Counter

Automated cell counter for trypan blue viability for cell lines

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Introduction to the Cellometer Auto T4 Bright Field Cell Counter

Cell Counting Made Simple

Automatic Cell Counting Made Simple with the Cellometer Auto T4

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Brightfield cell counting is recommended only for cell lines or purified/homogenous primary samples. Fluorescence cell counting is recommended for PBMCs, mouse splenocytes, hepatocytes, and other similar primary nucleated cell samples. To see our excellent line of fluorescent cell counters, please visit our fluorescent cell counters page.

Simple, Automated Cell Counting in Less than 10 Seconds

The Auto T4 utilizes bright field imaging and pattern-recognition software to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.

Cell counting results in less than 30 seconds

Load Sample, View Image, Count Cells, and Obtain Results in less than 10 seconds

The Auto T4 Cell Counter offers:

  • On screen view of individual cell level information

  • Ability to capture multiple fields of view for highly accurate data

  • Built-in cell type library

  • Count difficult cells (clumpy, irregular-shaped)

  • Options for IQ/OQ Validation and GMP/GLP software

One-step Concentration & Viability

Trypan blue viability with Cellometer Auto T4

The Auto T4 simultaneously calculates cell concentration and % viability for cultured cells stained with trypan blue.

Trypan blue viability is a dye exclusion method that utilizes membrane integrity to identify dead cells. The dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with bright field imaging.

Within 10 seconds, the Auto T4 instrument reports:

  • Live, dead, and total cell count
  • Live and total cell concentration
  • Mean Cell Diameter
  • % Viability

Cellometer Auto T4 instrument report

Counted bright field Image

Counted bright field Image from Cellometer Auto T4

Live cells = green circle
Dead (stained) cells = red circle

Analyze >99% of Cultured Mammalian Cells, including NCI-60 and clumpy MCF-7 cells

NCI-60 is a group of 59 human cancer cell lines (originally 60) developed by the National Cancer Institute for screening purposes. Isolated from various tissues (bone marrow, lung, ovary, brain, skin, breast, colon, kidney, prostate), the cell lines are used extensively to screen experimental cancer drug compounds and study tumor development.

Irregular-shaped Cells

Irregular-shaped Cell image from Cellometer Auto T4

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells and activated T-cells.

Clumpy Cell image from Cellometer Auto T4

Clumpy Cells

The MCF-7 breast cancer cell line can be very clumpy. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis (shown at left).

  • 57% of the NCI-60 cell lines are clumpy, contain debris, or display large variations in cell shape or size.
  • All 59 NCI-60 cell lines have been successfully validated on the Cellometer Auto T4 Cell Counter.

Cell Size Analysis & Size-based Counting

cell size histogram

The Auto T4 Cell Counter Automatically generates a cell size histogram based on cell diameter.

Because Cellometer generates individual cell size measurements, multiple samples can be overlaid on one histogram enabling analysis of the change in cell diameter over time.

Images from experiments involving SF9 or SF21 cells from the Cellometer Auto T4

For experiments involving SF9 or SF21 cells, saved images can be viewed and re-counted to analyze changes in morphology over time.

Cellometer auto T4 image showing cell size of dendritic cells

The minimum and maximum cell diameter settings can be optimized to count specific cells in a sample.

This example demonstrates counting of mature dendritic cells cultured from PBMCs based on cell diameter.

Multiple Cell Images for Data Verification

No two cells are the same. With the Auto T4 Cell Counter, cell morphology can be immediately viewed on-screen.

Two images with four fields of view each are captured per cell count. This is equivalent to the area of four quadrants of a hemocytometer. Counted cells are indicated on-screen for further verification that cells in the sample are being imaged and analyzed properly.

automatic cell-level data

Cellometer auto T4 imaging HEK293 and HeLa cells counted

Users can confirm that:

  • Cells are counted correctly, based on size and shape
  • Debris is excluded
  • Cells within clumps are being counted individually
  • Cell images can be archived and exported for use in publications and presentations.
  • Saved images can be re-counted using default or user-optimized analysis settings.

Exclusion of Debris

Exclusion of Debris from Cellometer Auto T4

Because Cellometer recognizes cells based on size, brightness, and morphology, cellular debris is easily and accurately excluded from counting results.

  • Cell size parameters can be modified to optimize exclusion of debris from results and enhance the accuracy of counting for a wide range of cell sizes.
  • The counted image can be viewed to verify exclusion of debris from results.

10x Faster and More Precise than Manual Counting

Hemacytometer under microscope, using Cellometer Auto T4

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto T4 Cell Counter calculates cell count and concentration for live and dead cells and % viability in just 10 seconds.

Cellometer Precision

The Cellometer %CV (Coefficient of Variation), including sample preparation, was well under 5% for most cell lines tested. The CVs for manual counts ranged from 7% to 20%. Automated cell counters remove inter-operator variability and subjectivity from cell counts to generate more precise data.

Cellometer Auto T4 vs Hemacytometer graph

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps
  • Eliminate Judgment Errors
  • Eliminate Recording & Calculation Errors
  • Reduce Counting Time … Run More Experiments
Cellometer Auto T4 Hemacytometer
Concentration n CV Concentration  n  CV
Jurkat 1.17 x 106 4 2.27% 1.11 x 106 3 8.04%
YAC-1 1.36 x 106 4 3.17% 1.41 x 106 3 11.89%
K562 6.36 x 105 4 8.29% 5.58 x 105 3 9.66%
H460 1.32 x 106 4 5.57% 1.16 x 106 3 20.65%
SW620 3.81 x 105 4 2.79% 3.70 x 105 3 19.49%
Hut 102 9.18 x 105 4 0.39% 7.68 x 105 3 10.60%

Optional GMP and GLP Support Software License

For laboratories that require QC access and audit controls, optional GMP/GLP software can be added to the Auto T4 Cell Counter

Features

  • Audit trail
  • Multi-layer access control
  • Auto printing by a custom template

Easily Print & Save Data and Cell Images

Export data to Excel

Easily Export to Excel for additional data formatting or sharing. Images and data tables can be pasted into PowerPoint presentations or submitted for publication.

Save data

Easily Save data and images to a network or local computer. View sample data file.

Print data

Print directly from the software screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. View sample report.

Counting Chambers — No Washing or Contamination

Cellometer auto T4 Counting Chambers

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged. This simple sample loading and analysis method is ideal for fragile cells.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

  • Time savings — no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume
  • Large-depth chambers for large cells
  • Most affordable automated counting consumables

Cellometer Auto T4 Accessories

IQ / OQ Validation Package

Installation Qualification (IQ) and Operational Qualification (OQ) package for the Auto T4 Cell Counter

Catalog # Description Size Unit
Cellometer Auto T4-IQOQ Counting chambers, reference bead solution, and validation protocol for the Cellometer Auto T4 100 counts 1 Set

On-Site Service, Maintenance, and Instrument Warranties

Trained Nexcelom Applications Specialists are available for on-site preventative maintenance, IQ validation, and OQ validation.

Service Description
Preventative Maintenance Available for all Cellometer Systems
Please inquire about scheduling and pricing.
IQ Validation* Available for Auto T4 and Vision Systems
Please inquire about scheduling and pricing.
OQ Validation* Available for Auto T4 and Vision Systems
Please inquire about scheduling and pricing.
Extended Warranty Available for all Cellometer Systems currently under a valid warranty.
Please inquire for pricing.
*IQ/OQ Validation Package must be purchased separately

Dedicated On-line and On-site Applications Support

Nexcelom Technical Support Specialists

Experienced Nexcelom Technical Support Specialists are available from 9am to 5pm EST for phone and on-line support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of a new Auto T4 Cell Counter

Help

The help button at the bottom right of the Cellometer Auto T4 software screen gives users instant access to:

  • Software features and instructions
  • On-line tutorials and training videos
  • Submission of a Support Ticket

Submit a Support Ticket

Clicking “Submit a Support Ticket” launches a convenient on-line form that is submitted directly to Nexcelom Technical Support

  • T4 instrument information fields are automatically populated
  • Images are easily attached for troubleshooting and applications support
  • A Nexcelom Specialist can assume remote control of the Auto T4 Cell Counter for troubleshooting and training

Nexcelom Field-based Applications Specialists

Nexcelom Field-based Applications Specialists are also available for:

  • On-site demonstrations
  • Training
  • Troubleshooting
  • Technical Seminars

All Nexcelom Applications Specialists are 100% focused on image-based cell concentration & viability and cell-based assays using Cellometer Image-Based Cytometry

Applications for Cellometer Auto T4 Bright Field Cell Counter

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines Counted with the Cellometer Auto T4

Cell Characteristics are based on observation in a Cellometer Counting Chamber. For adherent cell lines, cell characteristics are based on the appearance of cells following trypsinization.

786-O

786-0 cells

Origin: Kidney

Cell Characteristics: Round, normal size distribution

A498

A498 cells

Origin: Kidney

Cell Characteristics: Round, some size variation

A549/ATCC

A549/ATCC cells

Origin: Lung, NSCLC

Cell Characteristics: Round, normal size distribution, clusters > 5 cells

ACHN

ACHN cells

Origin: Kidney

Cell Characteristics: Mostly round, normal size distribution, clusters < 5 cells, debris

CAKI-1

CAKI-1 cells

Origin: Kidney

Cell Characteristics: Large variation in morphology

CCRF-CEM

CCRF-CEM cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

COLO 205

COLO 205 cells

Origin: Intestine

Cell Characteristics: Round, normal size distribution

DU145

DU145 cells

Origin: Prostate

Cell Characteristics: Round, normal size distribution, clusters < 5 cells

EKVX

EKVX cells

Origin: Leukemia

Cell Characteristics: Round cells, variable size distribution, clusters < 5 cells

HL-60

HL-60 cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

HOP-62

HOP-62 cells

Origin: Lung, NSCLC

Cell Characteristics: Round cells, some size variation, cell clusters < 5 cells

HOP-92

HOP-92 cells

Origin: Lung: NSCLC

Cell Characteristics: Round cells, some size variation

HS 578T

HS 578T cells

Origin: Breast

Cell Characteristics: Large variation in morphology, debris

HT-29

HT-29 cells

Origin: Intestine

Cell Characteristics: Round, normal size distribution, clusters < 5 cells

K562

K562 cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

LOX IMVI

LOX IMVI cells

Origin: Skin

Cell Characteristics: Large variation in morphology, debris

M-14

M-14 cells

Origin: Skin

Cell Characteristics: Round, some variation in size

MALME-3M

MALME-3M cells

Origin: Skin

Cell Characteristics: Round, normal size distribution

MDA-MB-231/ATCC

MDA-MB-231/ATCC cells

Origin: Breast

Cell Characteristics: Round, some size variation, some debris

MDA-MB-435

MDA-MB-435 cells

Origin: Breast

Cell Characteristics: Round, some size variation

MDA-MB-468

MDA-MB-468 cells

Origin: Breast

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

MOLT-4

MOLT-4 cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

NCI/ADR-RES

NCI/ADR-RES cells

Origin: Ovary

Cell Characteristics: Round, normal size distribution

NCI-H226

NCI-H226 cells

Origin: Lung, NSCLC

Cell Characteristics: Round, some size variation, some clusters < 5 cells

NCI-H23

NCI-H23 cells

Origin: Lung NSCLC

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

NCI-H322M

NCI-H322M cells

Origin: Lung, NSCLC

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

OVCAR-4

OVCAR-4 cells

Origin: Ovary

Cell Characteristics: Round, some size variation, clusters < 5 cells, some debris

OVCAR-5

OVCAR-5 cells

Origin: Ovary

Cell Characteristics: Round, some size variation, clusters < 5 cells

OVCAR-8

OVCAR-8 cells

Origin: Ovary

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

PC-3

PC-3 cells

Origin: Prostate

Cell Characteristics: Round, normal size distribution

RPMI 8226

RPMI 8226 cells

Origin: Leukemia

Cell Characteristics: Round, size variation

SF-268

SF-268 cells

Origin: Brain

Cell Characteristics: Round, some size variation, clusters < 5 cells

SF-295

SF-295 cells

Origin: Brain

Cell Characteristics: Round, some size variation, clusters < 5 cells

SF-539

SF-539 cells

Origin: Brain

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

SK-MEL-2

SK-MEL-2 cells

Origin: Skin

Cell Characteristics: Large variation in morphology and size

SK-MEL-5

SK-MEL-5 cells

Origin: Skin

Cell Characteristics: Variation in morphology and size

SK-MEL-28

SK-MEL-28

Origin: Skin

Cell Characteristics: Variation in morphology and size

SK-OV-3

SK-OV-3 cells

Origin: Ovary

Cell Characteristics: Variation in morphology and size, some clusters < 5 cells

SN-12C

SN-12C cells

Origin: Kidney

Cell Characteristics: Round, normal size distribution

SNB-19

SNB-19 cells

Origin: Brain

Cell Characteristics: Round, some size variation, clusters < 5 cells

SNB-75

SNB-75 cells

Origin: Brain

Cell Characteristics: Round, some variation in size and morphology

SR

SR cells

Origin: Lymphoma

Cell Characteristics: Large variation in morphology

SW620

SW620 cells

Origin: Intestine

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

T-47D

T-47D cells

Origin: Breast

Cell Characteristics: Large variation in morphology and size, some clusters < 5 cells

UACC-257

UACC-257 cells

Origin: Skin

Cell Characteristics: Large variation in size and morphology, some clusters < 5 cells

Performance of the Cellometer Auto T4 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N=20 Hemacytometer Auto T4
Average 1.03E+06 1.08E+06
STDEV 6.60E+04 1.01E+05
%CV 6.4% 9.4%

5 µm beads

N=20 Hemacytometer Auto T4
Average 1.07E+06 1.03E+06
STDEV 5.90E+04 6.46E+04
%CV 5.5% 6.3%

Models and Specifications for Cellometer Auto T4 Bright Field Cell Counter

Includes • Cellometer Auto T4 Instrument
• Cellometer Software
• USB 2.0 Connection Cable
• Power Supply
• Phone/online applications support during set-up
Available Accessories PC laptop
Imaging Performance Cell Size: 5 – 60 microns (most mammalian cells)
Conc. Range: 105 – 107 cells/mlBright field imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.
Instrument Specifications Weight: 10.4 lbs. (4.7kg)
Width: 3.5″ (8.9 cm)
Depth: 4.2″ (10.7 cm)
Height: 12.6″ (32.0 cm)Input to Power Adapter: 100-240 VAC, 50/60 Hz, 0.5A
Output to Instrument: 12 VDC, 1.5A
PC / Laptop requirements: (If purchasing Cellometer without PC laptop) • Windows 7, 8 or 10
• 1.6 GHz or higher processor
• 2GB RAM
• USB 2.0 port
• Display resolution: 1024 x 768 pixels or higherIntel Mac with Parallels/Windows

Customer Testimonials

Highly recommended from a colleague

The Auto T4 Cellometer is easy to set up and intuitive to use. Our lab is crowded and the compact size allowed us to squeeze it into the small amount of spare space we have. This counter came highly recommended from a colleague and so we had to get one for ourselves. It has made counting cells far more accurate and easier for us compared to our old counters and shared equipment.

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Highly recommended from a colleague

The Auto T4 Cellometer is easy to set up and intuitive to use. Our lab is crowded and the compact size allowed us to squeeze it into the small amount of spare space we have. This counter came highly recommended from a colleague and so we had to get one for ourselves. It has made counting cells far more accurate and easier for us compared to our old counters and shared equipment.

A time-saving machine

Cellometer [T4] has greatly reduced the time it takes to count the cells. There is a large library of settings for different type of cells. However, creating settings for a new cell type is very easy. Overall, its reliable and a time-saving machine.

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A time-saving machine

Cellometer [T4] has greatly reduced the time it takes to count the cells. There is a large library of settings for different type of cells. However, creating settings for a new cell type is very easy. Overall, its reliable and a time-saving machine.

User-friendly

Good Purchase. Cell counting has been made easier through the Cellometer Auto T4 instrument. The software is easy to access and user-friendly. Handling of the instrument is also user-friendly.

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User-friendly

Good Purchase. Cell counting has been made easier through the Cellometer Auto T4 instrument. The software is easy to access and user-friendly. Handling of the instrument is also user-friendly.

Great

It is easy to operate. It looks like a simple instrument, yet provides complete information of the cell counting: viable cells, dead cells, viability, total cell numbers. It also provides cell sizes and distribution that are very informative to help you know your cells better. This instrument is already trained for many commonly used cells in the lab. Great.

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Great

It is easy to operate. It looks like a simple instrument, yet provides complete information of the cell counting: viable cells, dead cells, viability, total cell numbers. It also provides cell sizes and distribution that are very informative to help you know your cells better. This instrument is already trained for many commonly used cells in the lab. Great.

Would definitely recommend

Cellometer Auto T4 has made my life easy. Now I don’t have to manually count the cell number on a neubauer chamber under the microscope and stress my eyes. Cellometer is very easy to use, fast and accurate. The best thing is that I can optimize the settings for counting different cells on the basis of size and it can even count clumpy cell lines. I would definitely recommend this instrument to everyone.

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Shweta Singh

Would definitely recommend

Cellometer Auto T4 has made my life easy. Now I don’t have to manually count the cell number on a neubauer chamber under the microscope and stress my eyes. Cellometer is very easy to use, fast and accurate. The best thing is that I can optimize the settings for counting different cells on the basis of size and it can even count clumpy cell lines. I would definitely recommend this instrument to everyone.

Shweta Singh

Very easy to use

The Auto T4 is very easy to use and the software is very friendly. I save a lot of time when I count number of cells and more when I am using more than one cell line.

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Very easy to use

The Auto T4 is very easy to use and the software is very friendly. I save a lot of time when I count number of cells and more when I am using more than one cell line.

The customer service is good too

We use [Cellometer] to count PBMC and tumor cells, Auto T4 is easy and good to use for measuring. The customer service is good too, willing to come and help set the optimization of the instrument. We recommend not only these machines, but this company also.

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The customer service is good too

We use [Cellometer] to count PBMC and tumor cells, Auto T4 is easy and good to use for measuring. The customer service is good too, willing to come and help set the optimization of the instrument. We recommend not only these machines, but this company also.

Unique features

We purchased [Cellometer] Auto T4 half-a-year ago, and were very happy with it. It has some unique features not avail on the other counters. I like the capability of the counter to show you which cells were counted. It is usually very accurate in picking up cells, including detecting individual cells in clusters and excluding all junk.

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Alexei Kisselev

Auburn University

Unique features

We purchased [Cellometer] Auto T4 half-a-year ago, and were very happy with it. It has some unique features not avail on the other counters. I like the capability of the counter to show you which cells were counted. It is usually very accurate in picking up cells, including detecting individual cells in clusters and excluding all junk.

Alexei Kisselev

Auburn University

Reproducible results

[Cellometer] Auto T4 is easy to use and allow us to get a reproducible result. We are satisfied with the function. It gets better if you have the autofocus function.

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Reproducible results

[Cellometer] Auto T4 is easy to use and allow us to get a reproducible result. We are satisfied with the function. It gets better if you have the autofocus function.

Speeds up my work significantly

The [Cellometer] Auto T4 is accurate and easy to use. It speeds up my work significantly. I love it!

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Ningwu Huang

Riboscience LLC

Speeds up my work significantly

The [Cellometer] Auto T4 is accurate and easy to use. It speeds up my work significantly. I love it!

Ningwu Huang

Riboscience LLC

Fast and reliable

The [Cellometer] Auto T4 is a fast and reliable way to count various types of cells and the option to export directly to Excel allows for easy E-notebook integration.

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Array Biopharma, Inc.

Fast and reliable

The [Cellometer] Auto T4 is a fast and reliable way to count various types of cells and the option to export directly to Excel allows for easy E-notebook integration.

Array Biopharma, Inc.

Very convenient and time-saving

The [Cellometer] Auto T4 we have in the lab is a very convenient and time-saving instrument, especially, when we have a lot of samples to quantify the cell number and cell viability. We like it very much!

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Sherleen Adamson

Very convenient and time-saving

The [Cellometer] Auto T4 we have in the lab is a very convenient and time-saving instrument, especially, when we have a lot of samples to quantify the cell number and cell viability. We like it very much!

Sherleen Adamson

Customer Publications using Cellometer Auto T4

Our customers include:

Cellometer Auto T4 customers