Protein-based therapy production requires optimized culture protocols

Media selection

Serum‐free cell culture media has undergone continuous development and optimization efforts with the goal of increasing cell density, viability, and recombinant protein titer [Ritacco et al. 2018].

Fine-tuning the media for your specific application can require testing different types and amounts of metabolites, nutrient amino acids, and antioxidants (e.g., DMSO). Considering the need for different concentrations and triplicate samples, media selection can quickly become an overwhelming task that is difficult to track. Image-based cytometry can monitor proliferation and viability to simply media selection.

The example above shows an experiment where three factors were used in different combinations (left panel). CHO cells (10/well) were plated on a 384-well plate (35 replicates per condition). On day 4, proliferation was measured with the Celigo platform, and the highest cell counts were observed for samples grown in media containing Factor C (right panel: 1, 5, 6, 7).

Nexcelom can rapidly and accurately determine the cell concentrations and viabilities in the multiple plates needed to perform these optimization experiments.

• Perform label-free cell proliferation assays in 96- and 384-well plates at 5 min per plate
• Direct cell counting to determine expression levels from 6- to 1536-well plates
• High speed, high-throughput, whole-well imaging

High-throughput cell counter provides precise cell count and viability for 24 samples in <3 min.

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