Monitoring growth and viability

The Cellaca MX high-throughput cell counter has been used to directly measure CHO cell concentration and viability in a 24-well chamber plate in less than 3 min for brightfield imaging with trypan blue staining. In addition, a Cell Fitness Panel kit can be used to measure 1) reactive oxygen species, 2) apoptosis with Annexin V and Caspase 3/7, and 3) viability and vitality using acridine orange/PI and calcein AM.

Growth tracking for single cell to single colony

The Celigo Image Cytometer can track single-cell growth to single colony. The images below show two examples of single cells in brightfield growing into colonies on 4 consecutive days.

single cell to single colony

Celigo software enables scientists to follow colony growth in the detected area.

colony growth day 3

Day 3

colony growth day 6

Day 6

colony growth day 10

Day 10

Cell growth tracking

After a cell line has been engineered, it is critical to monitor proliferation to characterize the line’s fitness and growth potential. The example below shows how the Celigo Image Cytometer can directly count individual cells or perform confluence measurements (bottom) in 96-well plates over time.

directly count individual cells

Celigo directly counts individual cells over time

measure cell confluence

Celigo performs confluence measurements over time