Video: Measure Apoptosis Using Caspase 3/7 for Suspension Jurkat Cells with Celigo Image Cytometer
Analysis of Cell Health on Celigo has Several Advantages:
- Easy-to-use interface to identify cell health
- Rapid scanning and analysis (scan and analyze a 96-well plate using 3 fluorescent channels in <12 min)
- Works in multi-well plate formats (1536-well to 6-well)
- Advanced flow cytometry-like gating
Quick assessment of cell health is performed using the Celigo cell imaging cytometer with its easy-to-use applications that allow the simple and rapid analysis of cell viability and apoptosis in many diverse cell types. The cell viability application uses calcein AM, propidium iodide and Hoechst to identify and count live, dead and total cell numbers, respectively. Similarly, the Phosphatidylserine (PS) Externalization application measures apoptosis by quantifying annexin V, propidium iodide and Hoechst to report apoptotic, dead and total cell counts, respectively.