Video: Measure Apoptosis Using Caspase 3/7 for Suspension Jurkat Cells with Celigo Image Cytometer

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Live Cell Analysis of Cell Health on Celigo has Several Advantages:

  1. Easy-to-use interface to identify cell health
  2. Rapid scanning and analysis (scan and analyze a 96-well plate using 3 fluorescent channels in <12 min)
  3. Works in multi-well plate formats (1536-well to 6-well)
  4. Advanced flow cytometry-like gating

Apoptosis Introduction

Quick assessment of cell health is performed using the Celigo cell imaging cytometer with its easy-to-use applications that allow the simple and rapid analysis of cell viability and apoptosis in many diverse cell types. The cell viability application uses calcein AM, propidium iodide and Hoechst to identify and count live, dead and total cell numbers, respectively. Similarly, the Phosphatidylserine (PS) Externalization application measures apoptosis by quantifying annexin V, propidium iodide and Hoechst to report apoptotic, dead and total cell counts, respectively.

Phosphatidylserine (PS) Externalization Application on Celigo Cell Imaging Cytometer

Image of Apoptotic Jurkat Cells & Concentration Response Curve

Image of Apoptotic Jurkat Cells & Concentration Response Curve

Representative image and concentration response curve of apoptotic Jurkat cells treated with camptothecin.

Live Cell Monitoring of Apoptotic Events Caspase 3/7

Kinetic measurement of caspase 3/7

Kinetic measurement of caspase 3/7 - apoptosis

Nexcelom ViaStain™ Live Caspase 3/7 kit is designed to detect caspase 3/7 activity in live cells. The ability to perform kinetic apoptosis assays allows researchers to continuously measure the caspase 3/7 activity within the cell population. This no wash assay has been shown to effectively work in both 2D and 3D cultures.