Fluorescent Assay Protocols & Demonstrations

Based on demonstrations we have conducted, as well as work we have done with current customers, we have put together three different types of documents.

Assay Overview

These documents provide a quick snapshot of how an assay could be performed on the Celigo, with some images and results of the assay. Download the complete Celigo Assay Overview eBook for all Assay Overviews.

Demonstration Experiment

These documents are created from actual experiments that have been conducted on the Celigo. The documents review how the experiment was done using the Celigo, and summarizes the results from the experiment. Download the complete Celigo Demonstration Experiment eBook for all Demonstration Experiments.

Application Protocol

These documents are instructional, and provide step-by-step details for customers to follow if they are running the assay on their Celigo, or for potential customers to review if they are setting up the experiments they will be conducting during the Celigo demonstration. For a copy of an Application Protocol document you see listed available here, please reach out to your local Applications Scientist, email us at support@nexcelom.com or call 978-327-5340

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Title Assay Overview

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Demo Experiment

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Application Protocol
Cell cycle analysis using DAPI and BrdU
Cell viability analysis using calcein AM, Hoechst and PI pdf
Endpoint apoptosis using Caspase 3/7 with Hoechst pdf pdf
Cell cycle analysis using EdU and DAPI pdf
Endpoint viability using DRAQ7 and Hoechst pdf pdf
Kinetic viability using DRAQ7 pdf pdf
High-throughput suspension cell count and viability using AO/PI pdf pdf
High-throughput suspension cell count and viability using AO/PI and BF
p53 and phosphor-p53 fluorescent marker analysis pdf
Endpoint viability using Propidium Iodide and Hoechst pdf pdf
Kinetic viability using PI pdf pdf
Kinetic apoptosis using Caspase 3/7 pdf pdf
Cell Cycle PI Analysis using A375 cells
HPC proliferation measurement using Ki-67 cellular marker pdf pdf
Antibody-Dependent Receptor Internalization Assay pdf
Count and measure cell infectivity of microcarriers pdf
Antibody Cell Surface Binding Detection pdf
GFP Transfection Efficiency Measurement pdf
Antibody-Dependent Drug Uptake Assay pdf
Kinetic pHrodo Antibody Internalization using Confluence pdf
Kinetic antibody internalization using pHrodo and bright field pdf
Endpoint antibody internalization using pHrodo and Hoechst pdf