High Quality Imaging and Cell Quantification for Cell Line Development

High Quality Imaging and Cell Quantification for Cell Line Development

Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biologics. Gene editing technologies are used to alter the function and to enhance production. During gene editing optimization and monitoring of stable clones, Celigo whole-well imaging allows for:

• Direct cell counting and quantification of transfection/transduction efficiencies

• Rapid high-throughput imaging platform

• Direct documentation and verification of single cell to a single colony

Assay Protocols & Demos

Single Colony Identification

Use bright field imaging to identify the formation of a single-cell clone colony.

Single Tumor Sphere Identification

Monitor and identify the formation of a single-cell clone tumor sphere.

Transfection/Transduction

Quantify transfection and transduction efficiencies by direct cell counting.

Single Cell Per Well Verification

Image a microwell plate to document the presence of a single cell in a well.