Measure Migration from 3D Spheroid onto ECM
Measure the Cell Outgrowth from a Spheroid onto an Extra Cellular Matrix (ECM)
- Directly image tumor spheroids in various microwell formats
- Non-invasive bright field imaging allows the user to image the same plate over multiple days
- Perform a two-color fluorescent viability assay
Introduction
The Celigo imaging cytometer has been developed to fully automate imaging and analysis of tumorspheres. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.
Measured Outgrowth from a HUVEC 3D Tumor Spheroid
Spheroid
Bright field image
Spheroid
Bright field image
Monitoring Cell Outgrowth from a GFP-labeled HUVEC Spheroid
Spheroid
Bright field image
Spheroid fill view
Bright field image
