Trehalose is a sugar disaccharide that supplies energy during cell cycle, or cell proliferation. Similar to neutral lipids, trehalose can protect yeast cells against stress, high ethanol content, heat, dehydration, oxidation, pH change. In order to measure trehalose content, the yeast cells are stained with concanavalin A-FITC (using FOM VB-535-402), and analyze fluorescence intensity of individual cells in a fluorescent histogram. Trehalose are also known to increase survival capacity during fermentation. The fluorescent linear gating was set to measure the population percentage of high FITC fluorescence.