Selected Cellometer Application Literature Review
Quantitative Approaches to Detect Donor and Passage Differences in Adipogenic Potential and Clonogenicity in Human Bone-Marrow-Derived Mesenchymal Stem Cells
Jessica Lo Surdo and Steven R Bauer
Tissue Engineering Part C: Methods
FDA/CBER/Cellular and Tissue Therapies Branch
Within a population of mesenchymal stem cells (MSCs), variability in cell properties such as proliferation, morphology, differentiation capacity, and cell surface marker expression proﬁles has been widely observed. Investigators are continually trying to improve cell characterization due to MSC heterogeneity. The MSCs plasticity (the ability to differentiate into many different cell types) may be due to the in vivo micro-environment, and long-term in vitro culturing. While qualitative and some quantitative approaches to assess MSCs from different donors currently exist, this paper discusses the development of robust quantitative measurements that can detect changes as a result of passaging, donor differences, and differences in subpopulations of MSCs. The overall goal of this work was to develop and employ quantitative measurements that will improve characterization MSCs by determining the role of donor variability and passage number as it relates to the biological properties and quality of MSCs
One such parameter tested was cell size. In this experiment, the cell size of MSCs was determined by diluting the cells 1:1 with trypan blue, and counting using an automated cell counter (Cellometer T4).
Mesenchymal Stem Cells were collected from two donors and the cell diameter of was measured after multiple passages. As the passage number increases, the cell diameter of MSCs from both donors increases. However, the change in cell size from the two donors was not uniform. These result shows that cell size is not only passage depended by also patient dependent.