Introduction to Surface and Intracellular Proteins Markers
Surface and intracellular proteins are markers that are important for identifying specific type of cells and characterizing cell populations. Traditionally, these assays are performed on flow cytometers. In the recent years, image-based cytometry method has been utilized for many standard surface and intracellular protein detection assay to provide a more simple and rapid method for cell population analysis. The Cellometer Vision CBA image cytometer is specifically designed to perform surface marker and intracellular protein fluorescence analysis to characterize cell populations. Below are examples of some of the assays have been performed using the Cellometer image cytometry method.
Human Activated T Cell – CD44-APC
Human T-cells were stained with APC conjugated anti-CD44 and imaged on the Cellometer Vision CBA. The counted bright field image shows T-cells automatically identified by the Cellometer software and outlined in green color. The fluorescent APC image shows red surface marker staining of positively stained T-cells. The mean fluorescent intensity values for each cell were exported to flow cytometry software FCS express and plotted as a histogram. The gated data shows that more than 95% of the cells were positive for CD44.