Human Clinical Samples
Bone Marrow (BM)
Bone Marrow (BM) is a complex tissue that is the site of hematopoiesis and B-cell development. Multipotent, non-hematopoietic stem cells, such as mesenchymal stem cells, may be isolated from human bone marrow. Furthermore, bone marrow mononuclear cells (MNCs) can be isolated by diluting the bone marrow sample and performing a density gradient separation. After centrifugation, the MNC layer is collected and may be further processed to isolate various subpopulations (hematopoietic progenitor cells, mesenchymal stem cells, CD33+ myeloid cells, and CD138+ plasma cells) using cell sorting.
Current Issue: Sample contamination with red blood cells may lead to inaccurate enumeration of immune cells.
Current Solution: Lyse red blood cells prior to cell count.
Nexcelom Solution: Perform fluorescent based analysis. Using AO/PI allows for the identification, enumeration of mononuclear cells, as well as provides cell viability in the counted sample.
Bone marrow samples can be processed and used for many different experimental applications such as studying hematopoietic cell differentiation pathways and acting as controls for BM malignancies studies.