Cytomegalovirus neutralization assay using different sera measured by counting fluorescently labeled infected cells
Detection of Alexa Fluor 647 (AF647)-labeled infected host cells
The example image below shows the low number of infected cells with high serum concentration in fluorescence using AF647.
Fluorescent images of AF647 labeled infected cells with high serum
The example image below shows the high number of infected cells with low serum concentration in fluorescence using AF647
Fluorescent images of AF647 labeled infected cells with low serum
The number of infected cells are counted and dose response curves are generated for 4 different sera, where the IC50 values are calculated. The Celigo is able to output the counted results directly to EXCEL for analysis
Plate-view of imaged and analyzed wells, and serum dilution-dependent neutralization plot in respect to the number of AF647-labeled infected cells
The Celigo Image Cytometer automates antibody neutralization assays:
Cytomegalovirus neutralization assay using fluorescence
The Celigo Image Cytometry system performs high-througput, whole-well imaging and quantitative data in bright field and up to four fluorescent channels for a wide variety of cell-based assays.
Learn more about modern virology assays:
