In this experiment, the goal is to measure the time-course CPE of the measles virus on the MRC-5 host cells in order to determine the earliest time point that CPE can be automatically detected.
The MRC-5 host cells are seeded in 6-well microplates, incubated and allowed to adhere overnight. The host cells are inoculated with the measles virus as the positive sample and media for the negative control. The 6-well microplates are scanned at 10% of the well that required less than 1 min/plate and analyzed using Celigo from day 0 to 14. The MRC-5 cell confluence percentages are measured directly in bright field imaging to determine the CPE.
Time-course confluence % results, showing the CPE occurring on day 8.
Example CPE measurement performed using the Celigo Image Cytometer: