Cytopathic effects of measles virus on MRC-5 cells measured by confluence percentages
In this experiment, the goal is to measure the time-course CPE of the measles virus on the MRC-5 host cells in order to determine the earliest time point that CPE can be automatically detected.
The MRC-5 host cells are seeded in 6-well microplates, incubated and allowed to adhere overnight. The host cells are inoculated with the measles virus as the positive sample and media for the negative control. The 6-well microplates are scanned at 10% of the well that required less than 1 min/plate and analyzed using Celigo from day 0 to 14. The MRC-5 cell confluence percentages are measured directly in bright field imaging to determine the CPE.
Time-course bright field and analyzed images (Confluence) for the positive measles virus. The reduction in confluence % of MRC-5 cells indicates cytopathic effects.
Time-course bright field and analyzed images (Confluence) for the negative media control.
Time-course confluence % results, showing the CPE occurring on day 8.
Example CPE measurement performed using the Celigo Image Cytometer:
CPE of measles virus on MRC-5 cells
Example CPE measurement performed using the Celigo Image Cytometer:
CPE of measles virus on MRC-5 cells
The Celigo Image Cytometry system performs high-througput, whole-well imaging and quantitative data in bright field and up to four fluorescent channels for a wide variety of cell-based assays.
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