Cytopathic effects of PI3 virus on Vero cells measured by confluence percentages
In this experiment, the goal is to measure the time-course CPE of PI3 virus on the Vero host cells in order to determine the earliest time point that CPE can be automatically detected.
The Vero host cells are seeded in 6-well microplates, incubated and allowed to adhere overnight. The host cells are inoculated with PI3 virus as the positive sample and media for the negative control. The 6-well microplates are scanned at 10% of the well that required less than 1 min/plate and analyzed using Celigo from day 0 to 12. The Vero cell confluence percentages are measured directly in bright field imaging to determine the CPE.
Time-course bright field and analyzed images (Confluence) for the positive PI3 virus. The reduction in confluence % of Vero cells indicates cytopathic effects.
Time-course bright field and analyzed images (Confluence) for the negative media control.
Time-course confluence % results, showing the CPE occurring on day 5.
Example CPE measurement performed using the Celigo Image Cytometer:
CPE of PI3 virus on Vero cells
Example CPE measurement performed using the Celigo Image Cytometer:
CPE of PI3 virus on Vero cells
The Celigo Image Cytometry system performs high-througput, whole-well imaging and quantitative data in bright field and up to four fluorescent channels for a wide variety of cell-based assays.
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