Measure Zika viral titration with mKate2 fluorescent protein reporter in 96-well plate
- Vero and Raji host cells are seeded separately in 96-well microplates and allowed to adhere overnight
- A titration of 2-folds of Reporter Virus Particles (RVP-Green) are prepared and added to the host cells
- The samples are allowed to incubate for 24 hours and then imaged and analyzed with Celigo image cytometer to count the number of infected cells
- The Celigo software was first used to identify the Raji cells in the bright field and Vero cells in the Bright field and Hoechst fluorescence channel
- Next, the GFP fluorescence positive infected cells were directly counted in the green channel
Bright field, fluorescent, and counted images of the total and infected Raji and Vero cells
The percent infectivity Celigo results for both Raji and Vero cells are directly compared to FACS showing highly comparable data
The Celigo Image Cytometer automates viral titer assays:
Zika viral titration with mKate2 fluorescent protein reporter
The Celigo Image Cytometry system performs high-througput, whole-well imaging and quantitative data in bright field and up to four fluorescent channels for a wide variety of cell-based assays.
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