Measure Zika viral titration with mKate2 fluorescent protein reporter in 96-well plate

  1. Vero and Raji host cells are seeded separately in 96-well microplates and allowed to adhere overnight
  2. A titration of 2-folds of Reporter Virus Particles (RVP-Green) are prepared and added to the host cells
  3. The samples are allowed to incubate for 24 hours and then imaged and analyzed with Celigo image cytometer to count the number of infected cells
  4. The Celigo software was first used to identify the Raji cells in the bright field and Vero cells in the Bright field and Hoechst fluorescence channel
  5. Next, the GFP fluorescence positive infected cells were directly counted in the green channel
zika viral titration

Bright field, fluorescent, and counted images of the total and infected Raji and Vero cells

percent infectivity zika virus

The percent infectivity Celigo results for both Raji and Vero cells are directly compared to FACS showing highly comparable data

The Celigo Image Cytometer automates viral titer assays: