After the freeze-thaw cycle, when viability drops, trypan blue's cytotoxic effect can further damage cells when incubation is required, thus skewing the sample's viability.
Everyone I meet that performs primary cell counting wants to optimize the amount of time they spend doing that task. They also agree that 30 seconds per count sounds pretty good.
Cellometer automated cell counters have been facilitating CAR T manufacturing with accurate cell counting and analysis since 2010.
How many brewers does it take to count yeast? This sounds like the beginning of a bad joke. In truth, cell counting is really simple but labor intensive & extremely time-consuming. With a Nexcelom Cellometer X2 – it only takes one click & 30 seconds per sample! Cellometer has earned a reputation as the “go to” cell counter for breweries seeking accurate yeast counts, viability & vitality. Just look at the map comparing the distribution of Nexcelom’s Cellometer automated yeast counters and the Top 50 breweries in the US. Data based on beer sales volume adapted from Brewery Association (BA) [...]