About Jean Qiu

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So far Jean Qiu has created 2 blog entries.

Use Cellometer Vision to measure nucleated cell concentration in whole blood without lysing RBC

Cellometer Vision incorporates image based cell counting and fluorescence detection in a compact and easy-to-use instrument. With fluorescence detection capabilities, Cellometer Vision is an ideal solution for many complex cell population characterization assays such as rapidly counting white-blood-cells in whole blood. Acridine orange (AO) is a fluorescent nucleic acid stain that has been used to measure nucleated cell concentration in various cell types, including mammalian cell lines, mammalian primary cells, and yeasts. AO is a membrane-permeable dye that stains nucleated cells green.   The following article describes a simple and specific immunoassay to detect cellular immune [...]

By |2013-03-29T10:07:38+00:00March 29th, 2013|Categories: Cellometer Application News|0 Comments

Measure total nucleated cells collected by Apheresis

Apheresis is the process of removing a specific component of the blood, such as platelets, red blood cells, plasma (liquid part of the blood) or granulocytes (white blood cells) and returning the remaining components to the donor. Leukapheresis is a specific kind of apheresis in which white blood cells  are separated from a sample of blood. Cells contained in a leukapheresis product is mainly nucleated cells, some residual red blood cells and platelets. Application: measure live /dead nucleated cell concentration and viability Sample Type: human peripheral blood Leukapheresis Cell Type: total nucleated cells Staining Method: acridine orange and propidium iodide (Ao/PI) Instrument: [...]

By |2012-05-01T13:13:06+00:00May 1st, 2012|Categories: Cellometer Application News|0 Comments