It's White Paper Wednesday! Read our featured white paper: Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer In this work, we demonstrate new applications of the Cellometer Vision for fluorescencebased cell population analysis as an alternative for flow cytometry. Cell cycle analysis was performed by inducing specific arrest in G0/G1, S, and G2/M phase of Jurkat cell population with aphidicolin, etoposide, and nocodazole, respectively [6-8]. The results were compared between the image-based and conventional flow cytometry methods. Download our white paper »
It's White Paper Wednesday! Read our featured white paper: Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer By developing fluorescent-based assays to assess mitochondrial membrane potential, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this work, we demonstrate a mitochondrial membrane potential assay using Cellometer imaging cytometry and the JC-1 dye as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry methods. Download our white paper »
Celigo Imaging Cytometer demonstrated the utility of automation in the development and monitoring of new CHO-based cell lines for increasing efficiency in cell line development.
Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision Cell concentration and viability of SVF preparations are usually determined by standard hemocytometer methods that are prone to considerable error since the operator must make judgments between actual cells versus "debris". To address that problem, we employed Cellometer image cytometry to perform both bright-field and fluorescence-based cell concentration and viability measurements . Here, we validated this method for SVF analysis. First, the imaging parameters were optimized by measuring five adipose SVF samples. Next, the concentration and viability of three [...]
A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer
It's White Paper Wednesday! Read our featured white paper: A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer In this study, Celigo was used to screen a compound library for effects on cell proliferation in adherent and non-adherent cell lines. Human lung carcinoma (A549) and promyelocytic leukemia (HL-60) cells were treated with a panel of compounds to inhibit proliferation. Finally, the Celigo system used image-based analysis to measure changes in cell morphology upon compound treatment. These data indicate that certain anti-proliferative compounds can have secondary effects on cell health or physiology, [...]
Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer
It's White Paper Wednesday! Read our featured white paper: Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer Cell proliferation is an important assay for pharmaceutical and biomedical research to test the effects of a variety of treatments on cultured primary cells or cell lines [1, 2]. Previously, we have reported a rapid and accurate fluorescence-based cell population analysis method using a novel image-based cytometry system. The method is highly comparable to traditional flow cytometry using fewer cells [3-6]. Here we report the development of a novel method for the kinetic measurement of cell [...]
It's White Paper Wednesday! Read our featured white paper: 3D Tumor Spheroid Analysis Method for HTS Drug Discovery using Celigo Imaging Cytometer U87MG cells were used to create tumorspheres in 384-well plates that were subsequently analyzed by imaging. The data illustrate that reproducible 3D spheroids can be formed in 384-well plates. Fluorescent viability studies were carried out with the imager using pixel intensity analysis. Moreover, the assay was validated for drug screen using various drug compounds that have shown anti-proliferative effects. Together, these data demonstrate that the tumorsphere formation assay can be developed, validated and used for high-throughput anti-cancer compound [...]
Avery Brewing Company in Boulder, Colorado has introduced a Cellometer to their brewing process, improving their procedures, as well as the product’s taste and quality. The Cellometer is employed as a quality control check over the yeast cells inside the facility’s fermenters. Making sure the yeast cells fermenting the beer are healthy and enzymatically active ensures a high quality product, with fewer mutated and/or dying cells that can negatively affect the beer’s flavor. Adam, the owner of Avery Brewing Co. says that the Cellometer has “changed the way we made beer and it also changed the way our beer tastes”. Watch this episode of [...]
Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision Recently, a new imaging cytometry system has been developed by Nexcelom Bioscience LLC (Lawrence, MA, USA) for fluorescence-based cell population analysis [1, 2]. Originally, the system has been used for automated cell concentration and viability measurement using disposable counting slides with both bright-field (BR) and fluorescent (FL) imaging methods . The system has recently been demonstrated to perform fast and accurate cell population analysis comparable to the traditional flow cytometry method. Download our white paper »
Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPSC reprogramming.