Celigo Imaging Cytometer demonstrated the utility of automation in the development and monitoring of new CHO-based cell lines for increasing efficiency in cell line development.
Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision Cell concentration and viability of SVF preparations are usually determined by standard hemocytometer methods that are prone to considerable error since the operator must make judgments between actual cells versus "debris". To address that problem, we employed Cellometer image cytometry to perform both bright-field and fluorescence-based cell concentration and viability measurements . Here, we validated this method for SVF analysis. First, the imaging parameters were optimized by measuring five adipose SVF samples. Next, the concentration and viability of three [...]
A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer
It's White Paper Wednesday! Read our featured white paper: A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer In this study, Celigo was used to screen a compound library for effects on cell proliferation in adherent and non-adherent cell lines. Human lung carcinoma (A549) and promyelocytic leukemia (HL-60) cells were treated with a panel of compounds to inhibit proliferation. Finally, the Celigo system used image-based analysis to measure changes in cell morphology upon compound treatment. These data indicate that certain anti-proliferative compounds can have secondary effects on cell health or physiology, [...]
Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer
It's White Paper Wednesday! Read our featured white paper: Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer Cell proliferation is an important assay for pharmaceutical and biomedical research to test the effects of a variety of treatments on cultured primary cells or cell lines [1, 2]. Previously, we have reported a rapid and accurate fluorescence-based cell population analysis method using a novel image-based cytometry system. The method is highly comparable to traditional flow cytometry using fewer cells [3-6]. Here we report the development of a novel method for the kinetic measurement of cell [...]
It's White Paper Wednesday! Read our featured white paper: 3D Tumor Spheroid Analysis Method for HTS Drug Discovery using Celigo Imaging Cytometer U87MG cells were used to create tumorspheres in 384-well plates that were subsequently analyzed by imaging. The data illustrate that reproducible 3D spheroids can be formed in 384-well plates. Fluorescent viability studies were carried out with the imager using pixel intensity analysis. Moreover, the assay was validated for drug screen using various drug compounds that have shown anti-proliferative effects. Together, these data demonstrate that the tumorsphere formation assay can be developed, validated and used for high-throughput anti-cancer compound [...]
Avery Brewing Company in Boulder, Colorado has introduced a Cellometer to their brewing process, improving their procedures, as well as the product’s taste and quality. The Cellometer is employed as a quality control check over the yeast cells inside the facility’s fermenters. Making sure the yeast cells fermenting the beer are healthy and enzymatically active ensures a high quality product, with fewer mutated and/or dying cells that can negatively affect the beer’s flavor. Adam, the owner of Avery Brewing Co. says that the Cellometer has “changed the way we made beer and it also changed the way our beer tastes”. Watch this episode of [...]
Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision Recently, a new imaging cytometry system has been developed by Nexcelom Bioscience LLC (Lawrence, MA, USA) for fluorescence-based cell population analysis [1, 2]. Originally, the system has been used for automated cell concentration and viability measurement using disposable counting slides with both bright-field (BR) and fluorescent (FL) imaging methods . The system has recently been demonstrated to perform fast and accurate cell population analysis comparable to the traditional flow cytometry method. Download our white paper »
Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPSC reprogramming.
Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation
It's White Paper Wednesday! Read our featured white paper: Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation Determining cell viability is a vital component in many biological experiments that range from standard cell culture to the investigation of pharmacological agents on tumor cells. One of the earliest and most common methods for measuring cell viability is the trypan blue (TB) exclusion assay [1, 2]. Over the last two decades, there have been various publications on comparing TB exclusion and fluorescence-based cellular viability assays [1, 3-5]. Previous results have shown that in a time-course measurement, TB exclusion [...]
It's White Paper Wednesday! Read our featured white paper: Automated Method for Determination of Infectious Dose (TCID50) using Celigo Imaging Cytometer Nexcelom's Celigo imaging cytometer has been applied to provide automated, rapid assessment of viral infectivity in a range of plate formats . Using f-theta optics, Celigo provides high quality, whole-well images using bright field and/or fluorescent illumination. Automated segmentation and analysis provide quantitative and objective output of CPE based on characteristic changes to the host cell monolayer. Download our white paper »