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So far Leo Chan has created 36 blog entries.

3D Tumor Spheroid Analysis Method for HTS Drug Discovery using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: 3D Tumor Spheroid Analysis Method for HTS Drug Discovery using Celigo Imaging Cytometer U87MG cells were used to create tumorspheres in 384-well plates that were subsequently analyzed by imaging. The data illustrate that reproducible 3D spheroids can be formed in 384-well plates. Fluorescent viability studies were carried out with the imager using pixel intensity analysis. Moreover, the assay was validated for drug screen using various drug compounds that have shown anti-proliferative effects. Together, these data demonstrate that the tumorsphere formation assay can be developed, validated and used for high-throughput anti-cancer compound [...]

By |2018-06-20T20:31:14+00:00July 8th, 2015|Categories: Celigo Application News|Tags: , , , |0 Comments

Craft brewers enhance brewing process and product taste using the Cellometer X1 and X2 instruments

Avery Brewing Company in Boulder, Colorado has introduced a Cellometer to their brewing process, improving their procedures, as well as the product’s taste and quality. The Cellometer is employed as a quality control check over the yeast cells inside the facility’s fermenters. Making sure the yeast cells fermenting the beer are healthy and enzymatically active ensures a high quality product, with fewer mutated and/or dying cells that can negatively affect the beer’s flavor. Adam, the owner  of Avery Brewing Co. says that the Cellometer has “changed the way we made beer and it also changed the way our beer tastes”.   Watch this episode of [...]

By |2015-06-30T10:18:52+00:00June 30th, 2015|Categories: Cellometer Product News|Tags: , , , , |0 Comments

Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision Recently, a new imaging cytometry system has been developed by Nexcelom Bioscience LLC (Lawrence, MA, USA) for fluorescence-based cell population analysis [1, 2]. Originally, the system has been used for automated cell concentration and viability measurement using disposable counting slides with both bright-field (BR) and fluorescent (FL) imaging methods [3]. The system has recently been demonstrated to perform fast and accurate cell population analysis comparable to the traditional flow cytometry method. Download our white paper »

By |2018-06-20T20:30:09+00:00June 24th, 2015|Categories: Cellometer Application News|Tags: , , |0 Comments

Non-Disruptive Quantification of 2° Reprogrammed iPS Colonies Using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Non-Disruptive Quantification of 2° Reprogrammed iPS Colonies Using Celigo Imaging Cytometer Nexcelom's Celigo S imaging cytometer has been applied to provide automated, rapid assessment of iPSC reprogramming. Using f-theta optics, Celigo provides high quality, whole well images using bright field and/or fluorescent illumination. Automated segmentation and analysis provides quantitative output of iPSC reprogramming based on mOrange and GFP fluorescent colony detection. Download our white paper »

Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation

It's White Paper Wednesday! Read our featured white paper: Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation Determining cell viability is a vital component in many biological experiments that range from standard cell culture to the investigation of pharmacological agents on tumor cells. One of the earliest and most common methods for measuring cell viability is the trypan blue (TB) exclusion assay [1, 2]. Over the last two decades, there have been various publications on comparing TB exclusion and fluorescence-based cellular viability assays [1, 3-5]. Previous results have shown that in a time-course measurement, TB exclusion [...]

Automated Method for Determination of Infectious Dose (TCID50) using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Automated Method for Determination of Infectious Dose (TCID50) using Celigo Imaging Cytometer Nexcelom's Celigo imaging cytometer has been applied to provide automated, rapid assessment of viral infectivity in a range of plate formats [4]. Using f-theta optics, Celigo provides high quality, whole-well images using bright field and/or fluorescent illumination. Automated segmentation and analysis provide quantitative and objective output of CPE based on characteristic changes to the host cell monolayer. Download our white paper »

By |2018-06-20T20:26:51+00:00May 6th, 2015|Categories: Celigo Application News|Tags: , , , , , |0 Comments

Characterization of Breast Cancer Drugs via Mammosphere Morphometric Analysis Using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Characterization of Breast Cancer Drugs via Mammosphere Morphometric Analysis Using Celigo Imaging Cytometer A panel of cytotoxic drugs, including doxorubicin and paclitaxel were used to study their effects on various breast cancer cell lines such as MDA-MD-436, MCF-7, SKBR3 and MDA-MB-231. Results show that the Colony Counting application can also be used to evaluate the clonogenicity and self-renewal of cancer stem/tumor-initiating cells by automatically analyzing mammosphere populations. The Colony Counting application on Celigo provides an efficient, reproducible and automated method for assessing the number, size, and morphology of cancer spheroids within [...]

By |2018-06-20T20:25:44+00:00April 22nd, 2015|Categories: Celigo Application News|Tags: , , |0 Comments

Measuring Physiological and Metabolic Characteristics of Yeast Health for Beer Fermentation Samples using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Measuring Physiological and Metabolic Characteristics of Yeast Health for Beer Fermentation Samples using the Cellometer Vision Characteristics such as the viability, vitality, glycogen, neutral lipid, and trehalose content of yeast samples must be measured to better understand changes in physiological and metabolic status during fermentation [4-9]. Monitoring changes of these parameters during fermentation can improve current production processes, and a subset of these parameters (glycogen, neutral lipid, and trehalose content) has been shown to play an important role in predicting yeast viability both during and after fermentation [10, 11]. Download our [...]

By |2018-06-20T20:24:40+00:00April 8th, 2015|Categories: Cellometer Application News|Tags: , , , , |0 Comments

Nexcelom’s Celigo Detects CRISPR Transfection Efficiency of sgRNA in CHO Cells

Introduction Chinese hamster ovary (CHO) cells are the cell type of choice for biopharmaceuticals due to their propensity to correctly fold and post-translationally modify human proteins [1]. Here, researchers use an optimized bioinformatics tool (“CRISPy”) to generate chimeric RNA called “single guide” RNA (sgRNA) in order to disrupt FUT8, a gene that is utilized in N-glycosylation. This novel web-based tool ensures efficient, fast, and low-cost genetic manipulation of CHO cells for future biopharmaceutical applications. Materials and Methods Cell culture and transfection of CHO cells CHO cells were cultured and transfected by Nucleofector Kit V with 1 μg of Cas9 plasmid [...]

By |2015-03-30T09:00:16+00:00March 30th, 2015|Categories: Celigo User Publications|Tags: , , , , |0 Comments

Comparing Fluorescence-Based Viability Detection Method using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Comparing Fluorescence-Based Viability Detection Method using the Cellometer Vision In this work, Cellometer Vision was employed to demonstrate rapid fluorescence-based viability measurements and the comparison of various fluorescent staining methods. First, fluorescent nucleic acid stains that examine membrane integrity were tested and validated by comparing against the standard trypan blue exclusion method. Similarly, fluorescent enzymatic stains that examine metabolic activities were tested and validated against the standard trypan blue exclusion method. Third, nucleic acid and enzymatic stains were compared by measuring viabilities of Jurkat cells incubated at different temperatures of water [...]

By |2018-06-20T20:22:26+00:00March 25th, 2015|Categories: Cellometer Application News|Tags: , |0 Comments