About Polly Mitchell

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So far Polly Mitchell has created 128 blog entries.

Counting of Patient-Derived IDC Organoids

Purpose To image and count whole-well populations of organoids in a rapid manner without having to take multiple, time consuming Z-stacked images Current Method(s) Manually, or Confocal Microscopy Target Cell Type Patient-derived intestinal differentiated cells (IDC) organoids Experiment Plan Organoids are cultivated in 6-well plates embedded in Matrigel and imaged in bright field Hypothesis Celigo will be able to perform rapid, whole-well imaging and analysis of organoids in a high throughput manner Celigo Setup Plate Type Corning 12-well microplate Scan Channels Bright field Resolution 1 µm/pixel Scan Area Whole well Analysis Method Tumorsphere 1 [...]

By |2018-09-21T21:15:21+00:00September 21st, 2018|Categories: Celigo Demo Experiments|Tags: |0 Comments

3D Multicellular Tumor spheroid Endpoint Apoptosis

Purpose Monitor the effects of a panel of drugs on the apoptosis of U87MG Glioblastoma MCTS using Caspase 3/7 and Hoechst fluorescent staining Current Method(s) Microscopy Target Cell Type U87MG Experiment Plan Allow U87MG spheroids to form and treat with a panel of drug compounds, then image on day 13 to measure the apoptotic effects of the compounds Hypothesis Using the bright field and fluorescent imaging, the Celigo will rapidly provide multicellular tumor spheroid images, and measure Caspase 3/7 and Hoechst fluorescent intensities of treated U87MG MCTS Celigo Setup Plate Type Nexcelom U-bottom Ultra-low [...]

By |2018-09-21T20:43:59+00:00September 21st, 2018|Categories: Celigo Demo Experiments|Tags: |0 Comments

3D Multicellular Tumor Sphere Invasion Screening

Purpose Monitor the effects of a panel of drugs on the invasion of U87MG Glioblastoma MCTS into Basement Membrane Extract (BME) Matrigel Current Method(s) Microscopy Target Cell Type U87MG Experiment Plan Allow U87MG spheroids to form and treat with a panel of drug compounds, then image at 0, 17, 23, 41, 47, and 68 hours after treatment to measurement the invasion inhibition effects of the compounds Hypothesis Using the bright field imaging, the Celigo will rapidly provide multicellular tumor spheroid invasion images and the area of the invasion of treated U87MG MCTS Celigo Setup [...]

By |2018-09-21T20:35:25+00:00September 12th, 2018|Categories: Celigo Demo Experiments|Tags: |0 Comments

3D Multicellular Tumor Spheroid Growth Inhibition Screening

Purpose Monitor the effects of a panel of drugs on the growth inhibition of U87MG Glioblastoma MCTS Current Method(s) Microscopy Target Cell Type U87MG Experiment Plan Allow U87MG spheroids to form and treat with a panel of drug compounds, then image on 0, 69, 114, 165, 210 hours post-treatment to measurement the growth inhibition effects of the compounds Hypothesis Using the bright field imaging, the Celigo will rapidly provide multicellular tumor spheroid images and diameters of treated U87MG MCTS Celigo Setup Plate Type Nexcelom U-bottom Ultra-low Attachment 384-well Plate (Cat# ULA-384U) Scan Channels Bright [...]

By |2018-09-21T20:36:43+00:00September 12th, 2018|Categories: Celigo Demo Experiments|Tags: |0 Comments

Label-Free Tumor Spheroid Growth Inhibition

Purpose To provide an automated solution that can provide images of multicellular tumor spheroids (MCTS) and report MCTS diameter in a 96-well format Current Method(s) Microscopy Target Cell Type NCI-H460, MiaPaca Experiment Plan Scan two plates daily to measure the size of multicellular tumor spheroids under different drug concentrations in hypoxic or normoxic conditions Hypothesis Using the bright field imaging, the Celigo will rapidly provide multicellular tumor spheroid images and diameters of treated MCTS in a 96-well plate Celigo Setup Plate Type Corning™ CLS70007 (ULA plate) - 96 well plate Scan Channels Bright field [...]

By |2018-09-21T20:39:18+00:00September 7th, 2018|Categories: Celigo Demo Experiments|Tags: |0 Comments

EACR Conference Series- Goodbye Flat Biology: In Vivo Inspired Cancer Biology and Therapy

9-12 September 2018 | Berlin, Germany Stand #3 11 September 12:10 INDUSTRY SYMPOSIUM - NEXCELOM BIOSCIENCE Somaieh Hedayat, The Institute of Cancer Research, UK "Patients derived organoids (PDOs) to model therapy response ex vivo"Somaieh Hedayat is working towards a PhD on how the tissue microenvironment can affect the response of metastatic colorectal cancer cells to antiangiogenic therapies. Prior to beginning her PhD, she spent 10 years working as a Higher Scientific Officer (HSO) in the Drug Discovery Unit on target validation and cancer biomarker projects. After gaining experience in in vivo and ex vivo biology, she decided to adjust her career path slightly [...]

By |2018-09-07T14:33:17+00:00September 7th, 2018|Categories: Upcoming Events|0 Comments

22nd Annual Merck Technology Symposium

Come visit us at booth #413 Merck Technology Symposium 2018 September 5-6, 2018 Ocean Place Resort Long Branch, NJ Posters being presented: High-throughput foci counting of viral titer and antibody neutralization assays using the Celigo Image Cytometer for developing a novel cross-reactive influenza vaccine Long-term time-course monitoring of NK cell-mediated ADCC using the Celigo Image Cytometer A high-throughput 3D tumor spheroid screening method for drug discovery using imaging cytometry

Immuno-Oncology Summit

August 27-31, 2018 | Boston, MA "Join over 600 thought leaders at industry’s leading Immuno-Oncology event and learn about the latest research in a comprehensive 12-track program, network and build lasting collaborations with an international mix of delegates from industry and academia, and gain actionable solutions to drive your organization’s next-generation immunotherapy programs." - www.immuno-oncologysummit.com Learn more

By |2018-07-13T14:57:28+00:00July 5th, 2018|Categories: Upcoming Events|0 Comments

Brewing Summit 2018

August 12-15, 2018 | San Diego, CA "It's been a busy four years and we're excited to see you all in sunny San Diego for Brewing Summit 2018! Master Brewers and ASBC join forces to bring you the most extensive lineup of leaders in the beer brewing industry. Discover all the latest insights and innovations—with engineering insights, new developments in quality procedures, recipes to try, technical sessions, networking events, and more. Whether you're currently working as a craft brewer or just starting out in your field, there are plenty of opportunities to advance your scope of technical knowledge at Brewing Summit [...]

By |2018-07-13T15:00:21+00:00July 1st, 2018|Categories: Upcoming Events|0 Comments