Purpose To image and count whole-well populations of organoids in a rapid manner without having to take multiple, time consuming Z-stacked images Current Method(s) Manually, or Confocal Microscopy Target Cell Type Patient-derived intestinal differentiated cells (IDC) organoids Experiment Plan Organoids are cultivated in 6-well plates embedded in Matrigel and imaged in bright field Hypothesis Celigo will be able to perform rapid, whole-well imaging and analysis of organoids in a high throughput manner Celigo Setup Plate Type Corning 12-well microplate Scan Channels Bright field Resolution 1 µm/pixel Scan Area Whole well Analysis Method Tumorsphere 1 [...]

Purpose Monitor the effects of a panel of drugs on the apoptosis of U87MG Glioblastoma MCTS using Caspase 3/7 and Hoechst fluorescent staining Current Method(s) Microscopy Target Cell Type U87MG Experiment Plan Allow U87MG spheroids to form and treat with a panel of drug compounds, then image on day 13 to measure the apoptotic effects of the compounds Hypothesis Using the bright field and fluorescent imaging, the Celigo will rapidly provide multicellular tumor spheroid images, and measure Caspase 3/7 and Hoechst fluorescent intensities of treated U87MG MCTS Celigo Setup Plate Type Nexcelom U-bottom Ultra-low [...]

Purpose Monitor the effects of a panel of drugs on the invasion of U87MG Glioblastoma MCTS into Basement Membrane Extract (BME) Matrigel Current Method(s) Microscopy Target Cell Type U87MG Experiment Plan Allow U87MG spheroids to form and treat with a panel of drug compounds, then image at 0, 17, 23, 41, 47, and 68 hours after treatment to measurement the invasion inhibition effects of the compounds Hypothesis Using the bright field imaging, the Celigo will rapidly provide multicellular tumor spheroid invasion images and the area of the invasion of treated U87MG MCTS Celigo Setup [...]

Purpose Monitor the effects of a panel of drugs on the growth inhibition of U87MG Glioblastoma MCTS Current Method(s) Microscopy Target Cell Type U87MG Experiment Plan Allow U87MG spheroids to form and treat with a panel of drug compounds, then image on 0, 69, 114, 165, 210 hours post-treatment to measurement the growth inhibition effects of the compounds Hypothesis Using the bright field imaging, the Celigo will rapidly provide multicellular tumor spheroid images and diameters of treated U87MG MCTS Celigo Setup Plate Type Nexcelom U-bottom Ultra-low Attachment 384-well Plate (Cat# ULA-384U) Scan Channels Bright [...]

Purpose To provide an automated solution that can provide images of multicellular tumor spheroids (MCTS) and report MCTS diameter in a 96-well format Current Method(s) Microscopy Target Cell Type NCI-H460, MiaPaca Experiment Plan Scan two plates daily to measure the size of multicellular tumor spheroids under different drug concentrations in hypoxic or normoxic conditions Hypothesis Using the bright field imaging, the Celigo will rapidly provide multicellular tumor spheroid images and diameters of treated MCTS in a 96-well plate Celigo Setup Plate Type Corning™ CLS70007 (ULA plate) - 96 well plate Scan Channels Bright field [...]