Channou Aing, Author at Nexcelom Bioscience

Spectrum Cell Counter – How to Easily Swap out the Interchangeable Filters

https://youtu.be/obIXdAvlN4E Video: Spectrum Cell Counter – How to Easily Swap out the Interchangeable Filters Learn about the various uses for the Cellometer Spectrum six interchangeable filter modules and see how fast and easy they are to swap out. Simply pop the old filter out, slide the new filter in, and open the software options to change the fluorescent module. The self-aligning Spectrum then optimizes the imaging background based on the specific filter for improved contrast and accuracy. Visit our ISO guidance pages to learn more about how cell counting methods impact the quality of results. Give us a call at [...]

By Channou Aing|2021-04-08T20:42:32+00:00April 8th, 2021|0 Comments

Poster: A Rapid, Label-Free, In Situ Assay Method for Cell Proliferation and Drug Toxicity

Download the Poster A Rapid, Label-Free, In Situ Assay Method for Cell Proliferation and Drug Toxicity PJ DiGregorio, M Zatcoff, S Kessel, I Valenta, and GR Bright Inhibition of cell proliferation is a sensitive marker of cytotoxicity. Manyproliferation assays use indirect measurements or require harvestingand/or staining of cells, analyzing only a sub-sample of the treated cells.These assays are destructive and do not allow kinetic measurements. TheCeligo™ Adherent Cell Cytometer is a novel, cell imaging product thatcombines whole well, in situ imaging with automated software for label-freebrightfield cell image analysis. In this study, Celigo was used to screen acompound library for [...]

By Channou Aing|2021-03-31T02:51:14+00:00March 31st, 2021|0 Comments

Poster: Concentration and Viability Measurement of Yeast in Corn Mash using the Cellometer Vision

Download the Poster Concentration and Viability Measurement of Yeast in Corn Mash using the Cellometer Vision Leo L. Chan, Emily J. Lyettefi, Alnoor Pirani, Tim Smith, Bo Lin, and Jean Qiu We demonstrate a novel imaging cytometry method for concentration and viability measurement of yeasts in corn mash directly from operating fermenters. It employs an automated cell counter, a proprietary dilution buffer and staining solution from Nexcelom Bioscience to enumerate yeasts in corn mash. This novel method provides an essential tool for biofuel industries in United States to efficiently monitor yeast viability during fermentation process to ensure consistent bioethanol output.

By Channou Aing|2021-03-31T02:44:30+00:00March 31st, 2021|0 Comments

Poster: Surface Marker Based Direct Cell Concentration Measurements Using Cellometer

Download the Poster Surface Marker Based Direct Cell Concentration Measurements Using a High Sensitivity Imaging Cytometry Method Leo L. Chan, Bo Lin, Peter Y. Li, and Jean Qiu Cell-based assays routinely employ sensitive fluorescence detection methods [1]. For simple cell counting, manual observation using a fluorescence microscope is time-consuming and prone to human error[2]. High content analysis such as confocal fluorescence microscopy can examine detailed cellular structures, but is not suitable for studying large cell populations. Common cell population analysis such as flow cytometry is fully automated and has high fluorescence sensitivity. Flow cytometers require calibration beads to produce cell [...]

By Channou Aing|2021-03-31T02:28:36+00:00March 31st, 2021|0 Comments

Poster: Automated Morphometric Analysis of Mammospheres

Download the Poster Automated Morphometric Analysis of Mammospheres: Characterization of Breast Cancer Drugs Olivier Dery, Sarah Kessel, Kristi Hohenstein Elliott, and Fredrik Kamme Mammospheres are commonly used in cancer research to studycancer pathogenesis1 and to identify new therapeutic agents2.Counting and morphometric analysis of suspension spherecultures, such as mammospheres, are frequently done manuallyand thus not amenable to high throughput applications. TheColony Counting application on the Celigo® cytometer provides accurate and consistent morphometric analysis of mammosphere populations in a non-destructive manner. The system records whole well images of multi-well (6-wells to 1536-wells) plates and allows for correlation of mammospheremorphology with anti-cancer drug [...]

By Channou Aing|2021-03-31T02:25:32+00:00March 31st, 2021|0 Comments

Poster: Comparison of fluorescence methods for determining yeast viability using Cellometer

Download the Poster Comparison of fluorescence methods for determining yeast viability using a novel automated image-based cell counting and viability system Alnoor Pirani, Benjamin Paradis, Leo L. Chan, Timothy Smith, Ning Lai, Bo Lin, Jean Qiu, and Peter Y. Li Yeast viability is an important parameter that can affect fermentationperformance in a brewery, which can dictate the quality of the end product.Traditional methods for determining viability involve either counting culturedyeast colonies on agar or counting methylene blue-stained yeast cells using ahemacytometer and a microscope. Faster and more robust technologies such as flow cytometry or absorbance plate readers involve the use [...]

By Channou Aing|2021-03-31T02:21:49+00:00March 31st, 2021|0 Comments

Poster: Cellometer for Quantitative Cell Viability Assay

Download the Poster A Novel Imaging Cytometry Method for Quantitative Cell Viability Assay Ning Lai, Leo L. Chan, Tim Smith, Alnoor Pirani, Hao Pan, Can Tan, Rebekah Harden, Jean Qiu, and Bo Lin We demonstrate a rapid and cost-effective method for viability analyses examining various characteristics of cell death using the Cellometer® Vision. This method eliminates many known issues caused by manual hemacytometer and flow cytometer. By using Cellometer Vision, the assay time for obtaining viability result is greatly reduced, which is significant forresearch development in academia and industry Current methods for viability analysis utilizes hemacytometer and standard flow cytometer. [...]

By Channou Aing|2021-03-31T02:16:38+00:00March 31st, 2021|0 Comments

Poster: Brightfield and Fluorescent Image Analysis for Screening Applications using Celigo

Download the Poster Brightfield and Fluorescent Image Analysis for Screening Applications using the Celigo Adherent Cell Cytometer Olivier Dery, Sarah Kessel, Kristi A. Hohenstein Elliott, Cory Peterson, and Fredrik Kamme The Celigo adherent cell cytometer is a multi-channel imagerwell suited for all image-based screening applications. Theflexibility and ease-of-use of the Celigo facilitates theoptimization of cell-based assays and the subsequentevaluation of assay performance. Using large field-of-viewoptics and a proprietary high speed imaging system, the Celigorapidly images multiple fields-of-view without moving the plate.Nearly all microplate formats (from 1536-well to 6-well plates)can be fully- or partially-imaged in brightfield and 3 fluorescentchannels. Additionally, unique [...]

By Channou Aing|2021-03-31T02:12:26+00:00March 31st, 2021|0 Comments

Poster: Kinetic Measurements of Rare Immune Cell Proliferation using Cellometer

Download the Poster A Novel Method for Kinetic Measurements of Rare Immune Cell Proliferation using Cellometer Image-Based Cytometry Leo L. Chan, Xuemei Zhong, Alnoor Pirani, Benjamin Paradis, and Bo Lin Measuring cell proliferation is important for studying the effects of varioustreatments on cultured primary cells or cell lines. Current proliferationanalysis methods employ flow cytometry for fluorescence detection of CFSElabeled cells. However, conventional flow cytometers require a considerable amount of cells per reading, which becomes an issue for kineticmeasurements with rare cell population due to the lack of sufficient samplesto test at multiple time points during the proliferation period. Here we [...]

By Channou Aing|2021-03-31T02:10:07+00:00March 31st, 2021|0 Comments

Poster: Rapid Quantification of Pathogenic Fungi by Cellometer

Download the Poster Rapid Quantification of Pathogenic Fungi by Cellometer Image-Based Cytometry Leo L. Chan, Charlotte Berkes, Alisha Wilkinson, and Benjamin Paradis The objective of this study was to develop an image-based cytometricmethodology for the quantification of viable pathogenic yeasts, which can offer increased sensitivity and efficiency when compared to the traditional colony forming unit (CFU) assay. Live/dead yeast quantification by flow cytometry has been previously demonstrated, however, adoption of flow cytometric detection of pathogenic yeasts has been limited for a number of practical reasons including its high cost and biosafety considerations. Our studies focus on detection of two human [...]

By Channou Aing|2021-03-31T02:04:33+00:00March 31st, 2021|0 Comments

About Channou Aing